Associations of MMP1, MMP2 and MMP3 Genes Polymorphism with Coal Workers’ Pneumoconiosis in Chinese Han Population
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Genotyping
2.3. Human MMP3 Levels in Serum Samples
2.4. Statistical Analysis
3. Results
3.1. Characteristics of the Study Population
Variables | CWP (n = 693) | Controls (n = 690) | p | |||
---|---|---|---|---|---|---|
N | % | N | % | |||
Age, Year (mean ± SD) | 68.0 ± 11.1 | 67.1 ± 8.4 | 0.113 | |||
Exposure years (mean ± SD) | 26.6 ± 9.0 | 27.3 ± 7.8 | 0.107 | |||
Smoking status | ||||||
Never | 340 | 49.1 | 359 | 52.0 | 0.270 | |
Ever | 353 | 50.9 | 331 | 48.0 | ||
Former | 162 | 23.4 | 91 | 13.2 | ||
Current | 191 | 27.5 | 240 | 34.8 | ||
Pack-years smoked | <0.001 | |||||
0 | 340 | 49.1 | 359 | 52.0 | ||
0–20 | 220 | 31.7 | 130 | 18.9 | ||
>20 | 133 | 19.2 | 201 | 29.1 | ||
Work type | 0.534 | |||||
Tunnel and coal mining | 659 | 95.1 | 648 | 94.0 | ||
Transport | 16 | 2.3 | 17 | 2.5 | ||
Others | 18 | 2.6 | 25 | 3.6 | ||
Stage | ||||||
I | 415 | 59.9 | ||||
II | 217 | 31.3 | ||||
III | 61 | 8.8 |
3.2. Associations between the Functional MMP Genes Polymorphisms and CWP Risk
Gene | rs No. | Location | Base Change | MAF | HWE a | Genotyping Rate (%) | |
---|---|---|---|---|---|---|---|
Case | Control | ||||||
MMP1 | rs1799750 | Promoter | G/- | 0.359 | 0.324 | 0.663 | 99.3 |
MMP2 | rs2285053 | Promoter | C > T | 0.225 | 0.236 | 0.596 | 99.2 |
MMP3 | rs522616 | Promoter | A > G | 0.340 | 0.372 | 0.117 | 98.7 |
Variables | CWP Cases | Controls | p a | OR (95% CI) | OR (95% CI) b | ||
---|---|---|---|---|---|---|---|
N | % | N | % | ||||
rs1799750 | n = 690 | n = 683 | |||||
2G/2G | 276 | 40.0 | 309 | 45.2 | 0.082 | 1.00 | 1.00 |
2G/1G | 325 | 47.1 | 305 | 44.7 | 1.19 (0.95–1.49) | 1.19 (0.95–1.49) | |
1G/1G | 89 | 12.9 | 69 | 10.1 | 1.44 (1.01–2.06) | 1.45 (1.02–2.07) | |
2Gallele | 877 | 63.6 | 923 | 67.6 | 1.00 | ||
1Gallele | 503 | 36.4 | 443 | 32.4 | 0.027 | 1.20 (1.02–1.40) | 1.20 (1.02–1.40) |
ADD | 0.026 | 1.20 (1.02–1.41) | |||||
DOM | 0.050 | 1.24 (1.00–1.54) | 1.24 (1.00–1.54) | ||||
REC | 0.104 | 1.32 (0.94–1.84) | 1.32 (0.94–1.85) | ||||
rs2285053 | n = 690 | n = 682 | |||||
CC | 409 | 59.3 | 395 | 57.9 | 0.684 | 1.00 | 1.00 |
CT | 252 | 36.5 | 252 | 37.0 | 0.97 (0.77–1.21) | 0.98 (0.79–1.23) | |
TT | 29 | 4.2 | 35 | 5.1 | 0.80 (0.48–1.33) | 0.79 (0.47–1.32) | |
C allele | 1070 | 77.5 | 1042 | 76.4 | 1.00 | ||
T allele | 310 | 22.5 | 322 | 23.6 | 0.477 | 0.94 (0.78–1.12) | 0.94 (0.79–1.13) |
ADD | 0.468 | 0.94 (0.78–1.12) | |||||
DOM | 0.610 | 0.94 (0.76–1.17) | 0.96 (0.77–1.19) | ||||
REC | 0.414 | 0.81 (0.49–1.34) | 0.80 (0.48–1.32) | ||||
rs522616 | n = 690 | n = 675 | |||||
AA | 301 | 43.6 | 276 | 40.9 | 0.130 | 1.00 | 1.00 |
AG | 309 | 44.8 | 296 | 43.8 | 0.96 (0.76–1.20) | 0.96 (0.76–1.20) | |
GG | 80 | 11.6 | 103 | 15.3 | 0.71 (0.51–0.99) | 0.70 (0.50–0.98) | |
A allele | 911 | 66.0 | 848 | 62.8 | 1.00 | ||
G allele | 469 | 34.0 | 502 | 37.2 | 0.081 | 0.87 (0.74–1.02) | 0.86 (0.74–1.01) |
ADD | 0.086 | 0.87 (0.75–1.02) | |||||
DOM | 0.307 | 0.89 (0.72–1.11) | 0.89 (0.72–1.10) | ||||
REC | 0.047 | 0.73 (0.53–0.99) | 0.72 (0.52–0.99) |
Variables | Cases/Controls | Genotypes (Cases/Controls) | p a | OR (95% CI) a | |||
---|---|---|---|---|---|---|---|
AA/AG | GG | ||||||
n | % | n | % | ||||
Total age | 690/675 | 610/572 | 88.4/84.7 | 80/103 | 11.6/15.3 | 0.041 | 0.72 (0.52–0.99) |
<68 | 275/397 | 246/335 | 89.4/84.4 | 29/62 | 10.6/15.6 | 0.199 | 0.72 (0.43–1.19) |
≥68 | 415/278 | 364/237 | 87.7/85.2 | 51/41 | 12.3/14.8 | 0.379 | 0.82 (0.52–1.28) |
Exposure years | |||||||
<27 | 268/261 | 236/216 | 88.1/82.8 | 32/45 | 11.9/17.2 | 0.060 | 0.62 (0.38–1.02) |
≥27 | 422/414 | 374/356 | 88.6/86.0 | 48/58 | 11.4/14.0 | 0.268 | 0.79 (0.52–1.20) |
Smoking status | |||||||
Never | 339/351 | 302/295 | 89.1/84.0 | 37/56 | 10.9/16.0 | 0.049 | 0.64 (0.41–1.00) |
Ever | 351/324 | 308/277 | 87.8/85.5 | 43/47 | 12.2/14.5 | 0.344 | 0.81(0.52–1.26) |
Stage | |||||||
I | 413/675 | 368/572 | 89.1/84.7 | 45/103 | 10.9/15.3 | 0.032 | 0.66 (0.45–0.96) |
II | 216/675 | 193/572 | 89.4/84.7 | 23/103 | 10.6/15.3 | 0.213 | 0.73 (0.44–1.20) |
III | 61/675 | 49/572 | 80.3/84.7 | 12/103 | 19.7/15.3 | 0.188 | 1.59 (0.80–3.18) |
3.3. Association and Stratification Analysis between MMP3 Expression and CWP Risk
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ji, X.; Wang, L.; Wu, B.; Han, R.; Han, L.; Wang, T.; Yang, J.; Ni, C. Associations of MMP1, MMP2 and MMP3 Genes Polymorphism with Coal Workers’ Pneumoconiosis in Chinese Han Population. Int. J. Environ. Res. Public Health 2015, 12, 13901-13912. https://doi.org/10.3390/ijerph121113901
Ji X, Wang L, Wu B, Han R, Han L, Wang T, Yang J, Ni C. Associations of MMP1, MMP2 and MMP3 Genes Polymorphism with Coal Workers’ Pneumoconiosis in Chinese Han Population. International Journal of Environmental Research and Public Health. 2015; 12(11):13901-13912. https://doi.org/10.3390/ijerph121113901
Chicago/Turabian StyleJi, Xiaoming, Lijuan Wang, Baiqun Wu, Ruhui Han, Lei Han, Ting Wang, Jingjin Yang, and Chunhui Ni. 2015. "Associations of MMP1, MMP2 and MMP3 Genes Polymorphism with Coal Workers’ Pneumoconiosis in Chinese Han Population" International Journal of Environmental Research and Public Health 12, no. 11: 13901-13912. https://doi.org/10.3390/ijerph121113901
APA StyleJi, X., Wang, L., Wu, B., Han, R., Han, L., Wang, T., Yang, J., & Ni, C. (2015). Associations of MMP1, MMP2 and MMP3 Genes Polymorphism with Coal Workers’ Pneumoconiosis in Chinese Han Population. International Journal of Environmental Research and Public Health, 12(11), 13901-13912. https://doi.org/10.3390/ijerph121113901