Re-Emergence of Dengue Serotype 3 in the Context of a Large Religious Gathering Event in Touba, Senegal
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Consideration
2.2. Real Time Case Notification and MBS-Lab Deployment
2.3. Patient Enrolment and Sample Collection
2.4. RNA Extraction
2.5. Dengue Virus Detection by qRT-PCR
2.6. Dengue Virus Antibody Detection by Serological Assays
2.7. Data Management and Real Time Case Reporting
2.8. Complete E Gene Sequencing
2.9. Dataset Phylogenetic and Phylogeographic Analysis
3. Results
3.1. Epidemiology, Demographic and Clinical Characteristics
3.2. Detection of DENV RNA and Serotyping
3.3. Detection of DENV IgM
3.4. Phylogenetic and Bayesian Analysis of Detected DENV-3 Strain
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Confirmed Cases (N = 263) | Negative (N = 569) | Total (N = 832) | p Value | |
---|---|---|---|---|
Age | <0.001 1 | |||
Median | 18 | 21 | 20 | |
Q1, Q3 | 9.5, 26.0 | 12.0, 32.0 | 12.0, 30.0 | |
Group age, in year | <0.001 2 | |||
Unknown | 12 | 27 | 39 | |
<15 | 91 (36.3%) | 165 (30.4%) | 256 (32.3%) | |
(15–30) | 115 (45.8%) | 204 (37.6%) | 319 (40.2%) | |
(30–45) | 32 (12.7%) | 114 (21.0%) | 146 (18.4%) | |
(45–80) | 13 (5.2%) | 59 (10.9%) | 72 (9.1%) | |
Sex | 0.111 2 | |||
F | 130 (49.4%) | 315 (55.4%) | 445 (53.5%) | |
M | 133 (50.6%) | 254 (44.6%) | 387 (46.5%) | |
Clinical signs and Symptoms | ||||
Fever | 213 (81.0%) | 369 (64.9%) | 582 (70.0%) | <0.001 2 |
Join pain | 154 (58.6%) | 326 (57.3%) | 480 (57.7%) | 0.732 2 |
Asthenia | 149 (56.7%) | 347 (61.0%) | 496 (59.6%) | 0.237 2 |
Headache | 243 (92.4%) | 495 (87.0%) | 738 (88.7%) | 0.022 2 |
Retrorbital pain | 60 (22.8%) | 123 (21.6%) | 183 (22.0%) | 0.698 2 |
Bleeding | 15 (5.7%) | 19 (3.3%) | 34 (4.1%) | 0.109 2 |
Muscle pain | 133 (50.6%) | 311 (54.7%) | 444 (53.4%) | 0.272 2 |
Abdominal pain | 73 (27.8%) | 168 (29.5%) | 241 (29.0%) | 0.601 2 |
Meningo-encephalitis | 8 (3.0%) | 11 (1.9%) | 19 (2.3%) | 0.320 2 |
Shock syndrome | 2 (0.8%) | 0 (0.0%) | 2 (0.2%) | 0.037 2 |
Rash | 10 (3.8%) | 16 (2.8%) | 26 (3.1%) | 0.4452 |
Profession | 0.256 2 | |||
Bureaucrat | 21 (8.0%) | 52 (9.1%) | 73 (8.8%) | |
Trade-market | 17 (6.5%) | 54 (9.5%) | 71 (8.5%) | |
Farmer | 6 (2.3%) | 21 (3.7%) | 27 (3.2%) | |
Student | 68 (25.9%) | 131 (23.0%) | 199 (23.9%) | |
Homemaker | 55 (20.9%) | 142 (25.0%) | 197 (23.7%) | |
Unspecific activities | 73 (27.8%) | 128 (22.5%) | 201 (24.2%) | |
Workers | 23 (8.7%) | 41 (7.2%) | 64 (7.7%) |
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Dieng, I.; Fall, C.; Barry, M.A.; Gaye, A.; Dia, N.; Ndione, M.H.D.; Fall, A.; Diop, M.; Sarr, F.D.; Ndiaye, O.; et al. Re-Emergence of Dengue Serotype 3 in the Context of a Large Religious Gathering Event in Touba, Senegal. Int. J. Environ. Res. Public Health 2022, 19, 16912. https://doi.org/10.3390/ijerph192416912
Dieng I, Fall C, Barry MA, Gaye A, Dia N, Ndione MHD, Fall A, Diop M, Sarr FD, Ndiaye O, et al. Re-Emergence of Dengue Serotype 3 in the Context of a Large Religious Gathering Event in Touba, Senegal. International Journal of Environmental Research and Public Health. 2022; 19(24):16912. https://doi.org/10.3390/ijerph192416912
Chicago/Turabian StyleDieng, Idrissa, Cheikh Fall, Mamadou Aliou Barry, Aboubacry Gaye, Ndongo Dia, Marie Henriette Dior Ndione, Amary Fall, Mamadou Diop, Fatoumata Diene Sarr, Oumar Ndiaye, and et al. 2022. "Re-Emergence of Dengue Serotype 3 in the Context of a Large Religious Gathering Event in Touba, Senegal" International Journal of Environmental Research and Public Health 19, no. 24: 16912. https://doi.org/10.3390/ijerph192416912