Energies 2013, 6(4), 2052-2064; doi:10.3390/en6042052
Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica
Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
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Received: 21 March 2013 / Accepted: 29 March 2013 / Published: 10 April 2013
Abstract
We entrapped lipase from Pseudomonas cepacia in polyallylamine-mediated biomimetic silica, and then applied entrapped lipase to the synthesis of biodiesel with soybean oil or waste cooking oil as a feedstock. The effects of reaction temperature, substrate molar ratio (methanol/oil) and n-hexane content (w/w of oil) were evaluated using response surface methodology (RSM) combined with Box-Behnken design. The optimal reaction conditions for soybean oil were 43.6 °C, substrate molar ratio of 4.3%, and 75% n-hexane. The predicted and experimental values of biodiesel conversion were 79% and 76%, respectively. The optimal reaction conditions for waste cooking oil were 43.3 °C, substrate molar ratio of 5%, and 38% n-hexane. The predicted and experimental values of conversion were 68% and 67%, respectively. The conversion efficiency remained the same even after 1-month storage of entrapped lipase at 4 °C or room temperature.Keywords:
biodiesel; lipase; biomimetic silica; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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MDPI and ACS Style
Kuan, I.-C.; Lee, C.-C.; Tsai, B.-H.; Lee, S.-L.; Lee, W.-T.; Yu, C.-Y. Optimizing the Production of Biodiesel Using Lipase Entrapped in Biomimetic Silica. Energies 2013, 6, 2052-2064.
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