Performance of Self-Collected Saliva Testing Compared with Nasopharyngeal Swab Testing for the Detection of SARS-CoV-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects and Specimens
2.2. Quantification of Viral Load
2.3. Statistical Analysis
3. Results
3.1. Characteristics of COVID-19 Patients
3.2. Comparison of Salivary and Nasopharyngeal SARS-CoV-2 Viral Load
3.3. Correlation between SARS-CoV-2 Viral Load and COVID-19 Symptoms
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | Sequences (5′-3′) | PCR Product |
---|---|---|
RdRp gen/nCoV_IP2 | 108 pb | |
nCoV_IP2-12669Fw 1 | ATGAGCTTAGTCCTGTTG | |
nCoV_IP2-12759Rv 1 | CTCCCTTTGTTGTGTTGT | |
nCoV_IP2-12669bProbe (+) 1 | [5′]HEX-AGATGTCTTGTGCTGCCGGTA-[3′]BHQ-1 | |
RdRp gene/nCoV_IP4 | 107 pb | |
nCoV_IP4-14059Fw 1 | GGTAACTGGTATGATTTCG | |
nCoV_IP4-14146Rv 1 | CTGGTCAAGGTTAATATAGG | |
nCoV_IP4-14084Probe (+) 1 | [5′]Fam-TCATACAAACCACGCCAGG-[3′]BHQ-1 |
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Carrouel, F.; Valette, M.; Perrier, H.; Bouscambert-Duchamp, M.; Dussart, C.; Tramini, P.; Bourgeois, D. Performance of Self-Collected Saliva Testing Compared with Nasopharyngeal Swab Testing for the Detection of SARS-CoV-2. Viruses 2021, 13, 895. https://doi.org/10.3390/v13050895
Carrouel F, Valette M, Perrier H, Bouscambert-Duchamp M, Dussart C, Tramini P, Bourgeois D. Performance of Self-Collected Saliva Testing Compared with Nasopharyngeal Swab Testing for the Detection of SARS-CoV-2. Viruses. 2021; 13(5):895. https://doi.org/10.3390/v13050895
Chicago/Turabian StyleCarrouel, Florence, Martine Valette, Hervé Perrier, Maude Bouscambert-Duchamp, Claude Dussart, Paul Tramini, and Denis Bourgeois. 2021. "Performance of Self-Collected Saliva Testing Compared with Nasopharyngeal Swab Testing for the Detection of SARS-CoV-2" Viruses 13, no. 5: 895. https://doi.org/10.3390/v13050895