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Viruses 2012, 4(9), 1792-1801; doi:10.3390/v4091792

PepGMV Rep-Protein Expression in Mammalian Cells

1
CA Ingeniería de Biosistemas, División de Investigación y Posgrado, Facultad de Ingeniería, Universidad Autónoma de Querétaro, Cerro de las Campanas s/n, Santiago de Querétaro, Qro., 76010, México
2
División de Investigación y Posgrado, Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Avenida de las Ciencias s/n, Juriquilla, Qro., 76230, México
*
Author to whom correspondence should be addressed.
Received: 27 July 2012 / Revised: 6 September 2012 / Accepted: 6 September 2012 / Published: 24 September 2012
(This article belongs to the Special Issue Plant Viruses)
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Abstract

The Geminiviruses genome is a small, single strand DNA that replicates in the plant cell nucleus. Analogous to animal DNA viruses, Geminiviruses depend on the host replication machinery to amplify their genomes and only supply the factors required to initiate their replication. Consequently, Geminiviruses remove the cell-cycle arrest and induce the host replication machinery using an endocycle process. They encode proteins, such as the conserved replication-associated proteins (Rep) that interact with retinoblastoma-like proteins in plants and alter the cell division cycle in yeasts. Therefore, the aim of this work is to analyze the impact of Pepper Golden Mosaic Virus (PepGMV) Rep protein in mammalian cells. Results indicate that the pTracer-SV40:Rep construction obtained in this work can be used to analyze the Rep protein effect in mammalian cells in order to compare the cell cycle regulation mechanisms in plants and animals. View Full-Text
Keywords: Geminivirus; cell cycle; Rep protein Geminivirus; cell cycle; Rep protein
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chapa-Oliver, A.M.; Mejía-Teniente, L.; García-Gasca, T.; Guevara-Gonzalez, R.G.; Torres-Pacheco, I. PepGMV Rep-Protein Expression in Mammalian Cells. Viruses 2012, 4, 1792-1801.

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