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Viruses 2013, 5(1), 321-339; doi:10.3390/v5010321

A Genotype of Modified Vaccinia Ankara (MVA) that Facilitates Replication in Suspension Cultures in Chemically Defined Medium

ProBioGen AG, Goethestr. 54, 13086 Berlin, Germany
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Received: 19 December 2012 / Revised: 11 January 2013 / Accepted: 17 January 2013 / Published: 21 January 2013
(This article belongs to the Section Antivirals & Vaccines)
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Abstract

While vectored vaccines, based on hyperattenuated viruses, may lead to new treatment options against infectious diseases and certain cancers, they are also complex products and sometimes difficult to provide in sufficient amount and purity. To facilitate vaccine programs utilizing host-restricted poxviruses, we established avian suspension cell lines (CR and CR.pIX) and developed a robust, chemically defined, culturing process for production of this class of vectors. For one prominent member, modified vaccinia Ankara (MVA), we now describe a new strain that appears to replicate to greater yields of infectious units, especially in the cell-free supernatant of cultures in chemically defined media. The new strain was obtained by repeated passaging in CR suspension cultures and, consistent with reports on the exceptional genetic stability of MVA, sequencing of 135 kb of the viral genomic DNA revealed that only three structural proteins (A3L, A9L and A34R) each carry a single amino acid exchange (H639Y, K75E and D86Y, respectively). Host restriction in a plaque-purified isolate of the new genotype appears to be maintained in cell culture. Processing towards an injectable vaccine preparation may be simplified with this strain as a complete lysate, containing the main burden of host cell contaminants, may not be required anymore to obtain adequate yields. View Full-Text
Keywords: modified vaccinia Ankara; MVA; vaccine; duck suspension cell line; AGE1.CR.pIX modified vaccinia Ankara; MVA; vaccine; duck suspension cell line; AGE1.CR.pIX
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Jordan, I.; Horn, D.; John, K.; Sandig, V. A Genotype of Modified Vaccinia Ankara (MVA) that Facilitates Replication in Suspension Cultures in Chemically Defined Medium. Viruses 2013, 5, 321-339.

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