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Viruses 2017, 9(2), 37; doi:10.3390/v9020037

Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis

1
State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering/Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, China
2
College of Pharmacy, Guizhou University, Guiyang 550025, China
3
College of Life Science, Guizhou University, Guiyang 550025, China
4
National Agricultural Extension Service Centre, Beijing 100026, China
5
Shidian Plant Protection Station, Shidian 678200, China
6
Mangshi Plant Protection & Quarantine Station, Mangshi 678400, China
*
Author to whom correspondence should be addressed.
Academic Editor: K. Andrew White
Received: 13 November 2016 / Revised: 18 January 2017 / Accepted: 18 February 2017 / Published: 22 February 2017
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
View Full-Text   |   Download PDF [7754 KB, uploaded 22 February 2017]   |  

Abstract

Diseases caused by southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt virus (RRSV) considerably decrease grain yield. Therefore, determining rice cultivars with high resistance to SRBSDV and RRSV is necessary. In this study, rice cultivars with high resistance to SRBSDV and RRSV were evaluated through field trials in Shidian and Mangshi county, Yunnan province, China. SYBR Green I-based quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to quantitatively detect virus gene expression levels in different rice varieties. The following parameters were applied to evaluate rice resistance: acre yield (A.Y.), incidence of infected plants (I.I.P.), virus load (V.L.), disease index (D.I.), and insect quantity (I.Q.) per 100 clusters. Zhongzheyou1 (Z1) and Liangyou2186 (L2186) were considered the most suitable varieties with integrated higher A.Y., lower I.I.P., V.L., D.I. and I.Q. features. In order to investigate the mechanism of rice resistance, comparative label-free shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS) proteomic approaches were applied to comprehensively describe the proteomics of rice varieties’ SRBSDV tolerance. Systemic acquired resistance (SAR)-related proteins in Z1 and L2186 may result in the superior resistance of these varieties compared with Fengyouxiangzhan (FYXZ). View Full-Text
Keywords: southern rice black-streaked dwarf virus; rice ragged stunt virus; resistance properties; field tests; quantitative real-time polymerase chain reaction; label-free shotgun LC-MS/MS proteomics southern rice black-streaked dwarf virus; rice ragged stunt virus; resistance properties; field tests; quantitative real-time polymerase chain reaction; label-free shotgun LC-MS/MS proteomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, Z.; Yu, L.; Jin, L.; Wang, W.; Zhao, Q.; Ran, L.; Li, X.; Chen, Z.; Guo, R.; Wei, Y.; Yang, Z.; Liu, E.; Hu, D.; Song, B. Evaluation of Rice Resistance to Southern Rice Black-Streaked Dwarf Virus and Rice Ragged Stunt Virus through Combined Field Tests, Quantitative Real-Time PCR, and Proteome Analysis. Viruses 2017, 9, 37.

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