Transcription Factors Mcm1 and Sfp1 May Affect [PSI+] Prion Phenotype by Altering the Expression of the SUP35 Gene
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmids
2.2. Strains
2.3. qPCR
2.4. Protein Analysis
2.5. Fluorescence Microscopy
2.6. Bioinformatic Analysis
3. Results
3.1. Transient Overexpression of MCM1 Enhances Nonsense Suppression in [PSI+] Strains
3.2. Search for the Potential Mcm1 and Sfp1 Binding Sites in the SUP35 and SUP45 Promoter Regions
3.3. The Potential Mcm1 and Sfp1 Binding Sites in the SUP35 Promoter Regions Do Not Significantly Influence Nonsense Suppression
3.4. The Effects of Transient MCM1 Overexpression Do Not Depend on the Potential Mcm1 Binding Sites
3.5. Mcm1 Does Not Form Detergent-Resistant Aggregates and Does Not Affect Sup35 Aggregation
3.6. The Potential Sfp1 Binding Site in the SUP45 Promoter Affects Its Expression and Nonsense Suppression
3.7. The Potential Sfp1 Binding Site in the SUP35 Promoter Is Important for [PSI+] Prion Toxicity
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
aa | amino acids |
bp | base pair |
GFP | green fluorescent protein |
ChIP | chromatin immunoprecipitation |
ChEC | chromatin endogenous cleavage |
5-FOA | 5-fluoroorotic acid |
TFBS | transcription factor binding site |
NLS | nuclear localization signal |
PQC | protein quality control |
RP | ribosomal protein |
RiBi | ribosome biogenesis |
PCR | polymerase chain reaction |
qPCR | quantitative polymerase chain reaction |
SDS-PAGE | sodium dodecyl sulfate–polyacrylamide gel electrophoresis |
SDD-AGE | semi-denaturating detergent agarose gel electrophoresis |
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Transcription Factor | Gene | TFBS Start (Rel. to ORF Start) | TFBS End (Rel. to ORF Start) | %Score |
---|---|---|---|---|
Mcm1 | SUP35 | −315 | −304 | 81.3% |
−281 | −270 | 82.7% | ||
−169 | −158 | 80.4% | ||
−40 | −29 | 75.6% | ||
SUP45 | −43 | −32 | 83.6% | |
−67 | −56 | 81.5% | ||
−165 | −154 | 76.4% | ||
−388 | −377 | 75.2% | ||
−435 | −424 | 76% | ||
−475 | −464 | 76.6% | ||
Sfp1 | SUP35 | −426 | −406 | 75.1% |
−328 | −308 | 81.9% | ||
−163 | −143 | 93.3% | ||
SUP45 | −20 | 1 | 77.3% | |
−174 | −154 | 92.6% |
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Matveenko, A.G.; Mikhailichenko, A.S.; Drozdova, P.B.; Zhouravleva, G.A. Transcription Factors Mcm1 and Sfp1 May Affect [PSI+] Prion Phenotype by Altering the Expression of the SUP35 Gene. Microbiol. Res. 2024, 15, 508-524. https://doi.org/10.3390/microbiolres15020034
Matveenko AG, Mikhailichenko AS, Drozdova PB, Zhouravleva GA. Transcription Factors Mcm1 and Sfp1 May Affect [PSI+] Prion Phenotype by Altering the Expression of the SUP35 Gene. Microbiology Research. 2024; 15(2):508-524. https://doi.org/10.3390/microbiolres15020034
Chicago/Turabian StyleMatveenko, Andrew G., Anastasiia S. Mikhailichenko, Polina B. Drozdova, and Galina A. Zhouravleva. 2024. "Transcription Factors Mcm1 and Sfp1 May Affect [PSI+] Prion Phenotype by Altering the Expression of the SUP35 Gene" Microbiology Research 15, no. 2: 508-524. https://doi.org/10.3390/microbiolres15020034