Anthracyclines Suppress Both NADPH Oxidase- Dependent and -Independent NETosis in Human Neutrophils
Abstract
:1. Introduction
2. Results
2.1. Drug Screening Shows That Anthracyclines Drastically Suppress NETosis
2.2. Anthracyclines Dose-Dependently Suppress Baseline NETosis
2.3. Anthracyclines Dose-Dependently Suppress Nox-Dependent NETosis without Affecting ROS Production
2.4. Anthracyclines Dose-Dependently Suppress Nox-Independent NETosis
2.5. Dexrazoxane, a Cardioprotective Agent, Does Not Alter the Suppressive Effect of Anthracyclines on NETosis
2.6. Doses of Anthracyclines That Fully Suppress NETosis Do Not Induce Apoptosis
3. Discussion
4. Materials and Methods
4.1. Research Ethics Board Approval
4.2. Reagents
4.3. Initial Screening Dosage Selection
4.4. Neutrophil Isolation
4.5. Sytox Green- NETosis Assay
4.6. DHR123- ROS Assay
4.7. Immunofluorescence Confocal Microscopy
4.8. Confocal Imaging of Apoptosis
4.9. Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Khan, M.A.; D’Ovidio, A.; Tran, H.; Palaniyar, N. Anthracyclines Suppress Both NADPH Oxidase- Dependent and -Independent NETosis in Human Neutrophils. Cancers 2019, 11, 1328. https://doi.org/10.3390/cancers11091328
Khan MA, D’Ovidio A, Tran H, Palaniyar N. Anthracyclines Suppress Both NADPH Oxidase- Dependent and -Independent NETosis in Human Neutrophils. Cancers. 2019; 11(9):1328. https://doi.org/10.3390/cancers11091328
Chicago/Turabian StyleKhan, Meraj A., Adam D’Ovidio, Harvard Tran, and Nades Palaniyar. 2019. "Anthracyclines Suppress Both NADPH Oxidase- Dependent and -Independent NETosis in Human Neutrophils" Cancers 11, no. 9: 1328. https://doi.org/10.3390/cancers11091328