Single-Cell Expression Landscape of SARS-CoV-2 Receptor ACE2 and Host Proteases in Normal and Malignant Lung Tissues from Pulmonary Adenocarcinoma Patients
Abstract
:Simple Summary
Abstract
1. Introduction
2. Methods
2.1. Lung Tissue Processing and Single-Cell RNA-Sequencing (scRNA-Seq)
2.2. scRNA-Seq Data Analysis
2.3. Statistical Analysis
3. Results
3.1. Single-Cell Decoding of ACE2 Expression and SARS-CoV-2 Priming Proteases in Lung Tissues of LUAD Patients
3.2. Expression Patterns of ACE2, TMPRSS2, and TMPRSS4 in Normal and Malignant-Enriched Epithelial Subsets
3.3. Genes Co-Expressed with ACE2 in Lung Epithelial Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Han, G.; Sinjab, A.; Hara, K.; Treekitkarnmongkol, W.; Brennan, P.; Chang, K.; Bogatenkova, E.; Sanchez-Espiridion, B.; Behrens, C.; Solis, L.M.; et al. Single-Cell Expression Landscape of SARS-CoV-2 Receptor ACE2 and Host Proteases in Normal and Malignant Lung Tissues from Pulmonary Adenocarcinoma Patients. Cancers 2021, 13, 1250. https://doi.org/10.3390/cancers13061250
Han G, Sinjab A, Hara K, Treekitkarnmongkol W, Brennan P, Chang K, Bogatenkova E, Sanchez-Espiridion B, Behrens C, Solis LM, et al. Single-Cell Expression Landscape of SARS-CoV-2 Receptor ACE2 and Host Proteases in Normal and Malignant Lung Tissues from Pulmonary Adenocarcinoma Patients. Cancers. 2021; 13(6):1250. https://doi.org/10.3390/cancers13061250
Chicago/Turabian StyleHan, Guangchun, Ansam Sinjab, Kieko Hara, Warapen Treekitkarnmongkol, Patrick Brennan, Kyle Chang, Elena Bogatenkova, Beatriz Sanchez-Espiridion, Carmen Behrens, Luisa M. Solis, and et al. 2021. "Single-Cell Expression Landscape of SARS-CoV-2 Receptor ACE2 and Host Proteases in Normal and Malignant Lung Tissues from Pulmonary Adenocarcinoma Patients" Cancers 13, no. 6: 1250. https://doi.org/10.3390/cancers13061250