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Review

Epidermal Growth Factor Pathway Signaling in Drosophila Embryogenesis: Tools for Understanding Cancer

by
Jay B. Lusk
1,
Vanessa Y. M. Lam
1 and
Nicholas S. Tolwinski
1,2,*
1
Division of Science, Yale-NUS College, Singapore 138527, Singapore
2
Department of Biological Science, National University of Singapore, Singapore 138615, Singapore
*
Author to whom correspondence should be addressed.
Cancers 2017, 9(2), 16; https://doi.org/10.3390/cancers9020016
Submission received: 14 December 2016 / Revised: 2 February 2017 / Accepted: 3 February 2017 / Published: 7 February 2017
(This article belongs to the Special Issue EGFR Family Signaling in Cancer)
Browse Figures
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Abstract

:
EGF signaling is a well-known oncogenic pathway in animals. It is also a key developmental pathway regulating terminal and dorsal-ventral patterning along with many other aspects of embryogenesis. In this review, we focus on the diverse roles for the EGF pathway in Drosophila embryogenesis. We review the existing body of evidence concerning EGF signaling in Drosophila embryogenesis focusing on current uncertainties in the field and areas for future study. This review provides a foundation for utilizing the Drosophila model system for research into EGF effects on cancer.

1. Introduction to EGF Signaling

1.1. Function of the EGF Receptor, Its Ligands, and an Antagonist

The Drosophila homolog of the epidermal growth factor receptor abbreviated as EGFR is known by several names—faint little ball and torpedo—with flb referring to the embryonic phenotype and torpedo referring to an oogenesis defect observed in eggs [1], DER and Ellipse for imaginal disc and adult eye phenotypes [2,3]. It is a single-pass transmembrane receptor tyrosine kinase (RTK), and is required for a variety of roles in normal Drosophila embryogenesis [4,5]. The extracellular domain of EGFR contains two cysteine-rich subdomains (SII and SIV), and two extracellular cysteine-poor subdomains (SI and SIII) [4]. Following a transmembrane domain, the intracellular portion of the protein is a protein tyrosine kinase. Drosophila EGFR is highly conserved with vertebrate forms of EGFR, sharing 40.5% identity [6,7,8].
EGFR receives its signal from a series of different ligands, which can interact with the receptor through different molecular mechanisms [9]. These ligands include gurken (grk), spitz (spi), vein (vn), and keren (krn) [10,11,12]. Three of the four ligands, grk, spi, and krn show the highest homology to TGF-α whereas vn is homologous to neuregulin [10]. It was previously proposed that different EGF ligands were responsible for different EGF responses [13], however recent evidence suggests that different patterns of expression may be responsible for different EGF signaling outcomes [14]. For example, the embryonic signaling function of vn can be substituted for by spi when expressed in the endogenous vn pattern, showing that in some cases the identity of the ligand is not as critical compared to the ability of the receptor to bind the signal [10].
krn is less well-characterized than spi. Emerging literature appears to suggest that, aside from its alternative mechanism of transport and regulation, it is a functional homologue to spi, which functions redundantly with spi in early development [10,15,16]. However, the functional redundancy of krn in embryogenesis lends itself to a question with no immediately compelling answer: what selective pressure would cause krn to be maintained as a ligand in EGF signaling, and why would it develop an alternative cleavage mechanism to the primary ligand, spi.
vn also appears to be biochemically redundant with the TGF-α-like ligands. In fact, loss of vn in lateral embryonic stripes had no effect on downstream activation of ERK [17]. vn expression is determined by the gradient of nuclear Dorsal established by the Toll pathway [17], but vn’s unique function may be to activate the EGF signal in tissues where low levels of activation are required due to its weaker activation capacity than spi [14,18]. spi is able to rescue vn mutants and modulates its activity to roughly match the activation by vn [10]. Overall, these three types of ligands which serve similar functions, allow the EGF pathway to signal in a variety of conditions increasing its accuracy and specificity in effecting cellular changes in response to other developmental events.
In addition to these four extracellular ligands, Argos (arg) serves as a ligand antagonist by forming a clamp-like structure around spi and thereby inhibiting EGF signal transduction [9]. arg functions in the pathway through a negative feedback loop where it is produced as a result of high levels of EGFR activity [19]. arg is required for normal function of the EGF pathway, and loss of Arg results in excessive EGFR pathway activation and corresponding expansion of ventral cell fates [19]. arg therefore acts to limit long-range effects of the signal by restricting the spread of spi [20].
Some recent experiments suggest that the EGFR ligands are interchangeable as there is significant redundancy between ligands, similarity in mechanisms of activation, and minimal differences in regulation between the ligands [10,16,18,21]. Instead, it seems that differing quantitative transduction of signal due to differences in ligand concentration (and activation capacity), is responsible for the differentiation of EGF receiving tissues. What this means is that each ligand is capable of activating a specific amount of signal transduction, where tissues receiving the EGF signal differentiate according to the strength of signaling. There is no compelling explanation for how selective pressure led to the evolution of several redundant mechanisms. It is possible that the broad diversity and critical nature of EGF signaling both in development and in a wide range of other biological processes has influenced the development of redundant ligands with subtly differing regulatory mechanism or strength. It is also possible, especially given the similarity of spi, krn, and grk to TGF-α, and vn’s corresponding dissimilarity, that any of these ligands were co-opted from another function early in evolution, and the original function was later lost, with the ligand taking on a new role as a redundant regulator of the EGF signal. The finding that arg binds to the EGF receptor, not the ligand, and in a manner different from the ligands, is consistent with the idea that several ligands were co-opted from other functions while the inhibitory ligand (of which only one exists) was central to the function of the receptor prior to the evolutionary events where other ligands were co-opted [22].

1.2. Intracellular Regulation of EGF Ligands

EGF ligands undergo significant intracellular regulation in the process of their production. SPI is initially produced as a transmembrane pro-protein, which is then cleaved to release the extracellular signaling domain [11]. SPI is cleaved into the endoplasmic reticulum (ER), and trafficked by bulk flow to the Golgi [23,24,25]. In the absence of STAR, a ubiquitously-expressed type II single domain transmembrane protein, which binds to the inactive form of SPI, it will undergo retrograde trafficking back to the ER [26]. Formation of the STAR-SPI complex will inhibit retrograde trafficking from the Golgi to the ER [24,25]. The STAR-SPI complex is then trafficked to an endosomal compartment where Rhomboid1 (RHO-1) cleaves SPI, yielding the active ligand which can now be secreted to the extracellular space, and the STAR-SPI and RHO-SPI complexes disassociate [24,25,27,28]. Interestingly, KRN is capable of undergoing low levels of cleavage and subsequent EGF activation in the absence of rho-1/star-mediated trafficking, despite its otherwise identical function to SPI in terms of activating the EGF signaling pathway [15].
rho-1 deserves particular attention as a regulatory protein within the EGF signaling pathway. Not only is rho-1 very highly conserved [21], but it also has been shown to specifically act in trafficking of EGF ligands [25]. All four EGF ligands and STAR are substrates for rho-1 mediated cleavage [24]. While the regulatory action of rho-1 on EGF extracellular ligands is clear, it is still unclear what, if any, regulatory function RHO-1 has on STAR [25]. Both star and spi are ubiquitously expressed, whereas rho-1 is precisely spatially and temporally regulated [17,29], which has key influence on spatial transduction of the EGF signal. Therefore, to understand more precisely the mechanism through which RHO-1 regulates EGF signaling, an understanding of the regulatory mechanisms Rho-1 has on STAR is needed. Overall, Rho-1 is understood to be the primary effector of the EGF response, due to its ability to respond both to positive feedback and to induce EGF signaling in neighboring cells in oogenesis [25]. This mechanism is likely to be seen in embryonic development as well, exerting its effect through spi rather than grk. RHO-1 is a key point of cross-pathway regulation in early embryogenesis, which will be discussed below.

1.3. Transduction of the EGF Signal from the Receptor to ras

Drosophila was originally thought to have three homologs of vertebrate ras, a gene at 85D based on chromosome location (ras85D or ras1), the second gene at 64D (ras64D or ras2), and rap1 (ras3) (which was later found to be a separate GTPase and not in fact a ras homolog). We will focus on Ras1 as it is most applicable to embryonic patterning [30,31]. ras1 has been studied extensively in EGF—mediated signaling in the Drosophila eye and in higher organisms, and we will apply many of the structural findings which have elaborated the molecular mechanisms through which ras1 transduces the EGF signal to the later components of the pathway in embryogenesis.
The basic outcome of EGF signal transduction is the activation of RAS. The actual mechanism is more complex. First, upon ligand binding, EGFR forms a dimer and trans-phosphorylates across the dimer. The protein Downstream of Receptor Kinase (DRK, the Drosophila homolog of mammalian Grb2) binds through its SH2 domain to the phosphorylated tyrosines on EGFR localizing it to the plasma membrane. In turn, DRK binds Son of Sevenless (SOS) the guanine exchange factor which triggers activation of RAS by promoting GTP binding [20,32].
RAS is therefore positively regulated by the EGF. It is also negatively regulated by sprouty (sty). sty was once thought to be an extracellular inhibitor of EGF signaling [33], but recent data suggests that sty functions as an intracellular inhibitor of the pathway modulating ras activity [34]. While the precise mechanism through which sty regulates the pathway is not clear, evidence suggests that STY interacts with DRK and GAP1, at least in eye development [14]. This interaction between STY and DRK was suggested to prevent the interactions of DRK with SOS and other accompanying activating factors. Understanding how sty functions will lead to a full molecular mechanism of signal transduction from EGF to ras [14].

1.4. Mechanism of RAS Activation of RAF and the Kinase Cascade

The main function of active RAS is to activate D-RAF (also known as polehole in Drosophila or just RAF), a highly conserved serine-threonine protein kinase. The fundamental function of activated RAS is to localize RAF to the plasma membrane, where RAF undergoes further activation [35,36]. RAF has three conserved regions, the first binds to RAS, the second is the negative regulatory domain, and the third is the kinase [37]. After RAF is activated by RAS, RAF phosphorylates mitogen-activated Protein kinase kinase (MAPKK, MKK), also known as also known as mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) Kinase (MEK). MEK was originally known in Drosophila as downstream of raf-1 (dsor1), and was shown to have significant sequence similarity to murine MEK and Xenopus MAP Kinase activator. MEK was shown to be activated through direct phosphorylation by RAF, causing activation of kinase activity [38,39,40]. After MEK is activated by RAF it phosphorylates the Drosophila homolog of MAP kinase (MAPK)/extracellular signal regulated kinase (ERK), known as rolled [40,41]. Among many targets, activated rolled phosphorylates the transcription factors such as pointed and yan which in turn lead to transcriptional responses [42]. Cells with a loss of function in rolled, as expected, produce the same cell-death phenotype as seen in an EGF loss of function [43].
In embryogenesis, pointed is responsible for most of EGF’s effects on cell differentiation [44,45]. pointed has two promoters, allowing expression of two different transcript forms, which can in some cases show partial heteroallelic complementation [44]. A second rolled target is yan (also known as anterior open), a transcription factor responsible for inhibiting cell differentiation. In essence, the two transcription factors are in opposition with pointed as the activator of EGF pathway target genes and yan as the repressor.
When the EGF signaling pathway is activated, yan is destabilized and therefore repressed, and pointed is activated [30]. The combined effect of yan derepression and pointed activation results in activation of EGF target genes such as orthodenticle (otd), arg, and tartan (which effect a wide variety of changes in different tissue [19]. Figure one summarizes the entire EGF signaling pathway (Figure 1).

2. Patterning of the Neuroectoderm

Three homeobox genes control the development of the dorsal-ventral axis of the Drosophila central nervous system: muscle segment homeobox (msh), ventral nervous system defective (vnd), and intermediate neuroblasts defective (ind) [46]. This subdivision is mediated by toll, dpp, and EGF signaling, which together are responsible for establishing three separate Hox domains. Specifically, where the msh domain is patterned due to repression of dpp, ind is expressed after being activated both by Toll and EGF signaling, and the vnd domain is established by Toll signaling and maintained by EGF signaling [46,47,48]. This process works through a repressive function of dpp signaling [49], and shows that EGF signaling works both to repress dpp signaling and to preserve the existing dorsal-ventral axis established by early Toll signaling [50,51,52]. While this model is highly compelling on a basic level, recent evidence has shown that development of the neuroectoderm depends on a vast web of connecting repressive and activating networks. Far from acting independently of one another, the three homeobox genes repress each other (through ind repression of vnd), and that EGF over expression causes the expansion of both vnd and ind into the lateral domain (originally msh-expressing domain) [53]. Recently, evidence has also emerged suggesting that EGF signaling is necessary for glial cell development, supporting the argument that EGF plays a more significant role in cell fate determination in the developing neuroectoderm. Kim et al. found that glial markers such as gcm and repo were reduced in the absence of EGF or spi, concluding that EGF signaling is responsible both for initial formation and maintenance of lateral glia. Likewise, over expressing EGF through over activated spi caused an expansion of the medial and intermediate cell fates into the lateral cell column, causing a repression of Repo [54]. This indicates that EGF has both necessary repressive and activating functions in specification of ventral nervous system cell fates, mediated by complex interactions with components of other signaling pathways. For example, while EGF was originally thought to only repress dpp, recent work shows that EGF could also be repressed by dpp. While dpp primarily functions at a short range, bounded by interaction with EGF and other pathways, dpp can also function indirectly at long-range in neuroblast development by repressing EGF signaling, again demonstrating the significant versatility of EGF signaling as a regulatory mechanism for precise spatial and temporal differentiation of the neuroectoderm [55].
Furthermore, we now know that EGF signaling has at least some degree of ability to pattern the neuroectoderm independently of its genetic effect on key neural development homeobox genes. EGF signaling has been shown to mediate the direct phosphorylation of ind through its downstream effector MAP kinase. Phosphorylation of ind results in repression of ind target genes, which allows EGF signaling to modify cell fate even in the presence of high levels of ind protein [56].
This finding strengthens the conclusion that EGF signaling is necessary for neural development, as an active regulator of key homeobox genes and their protein products. This is further supported by the fact that the loss of EGF signaling results in widespread apoptosis of alternative neuroblast identities [57]. Finally, the regulatory mechanisms thought to be common to the diverse functions of the pathway (i.e., argos-mediated repression of EGF ligand binding) allows EGF signaling to maintain precise gradients of cell fate determination in the lateral regions of the ventral neuroectoderm [58]. Figure two summarizes the signaling events involved in patterning of the neuroectoderm (Figure 2).

3. Non-Neuroectodermal Ventral Patterning, Including Segmentation

EGF signaling is also involved in ventral epidermal patterning, where it interacts with wnt and hedgehog signaling in the patterning of denticles. There are two ways in which WNT and EGF signaling interact [59,60,61]. First, EGF signaling induces denticle cell fates through the transcription factor shavenbaby [62]. WNT signaling blocks EGF signaling and permits cells to develop the smooth cuticle cell fate [61,62], but in a complicated twist EGF is also required for the survival of smooth cuticle cells [61,63,64]. This is very similar to EGF signaling in the neuroectoderm where it is both a repressor of certain alternate neuroblast cell fates and requisite for the survival of most types of neuroblasts (see the above section). Second, downstream of raf-1 (Dsor1, the Drosophila homolog of MEK1/2) has been shown to inhibit AXIN-mediated destruction of Armadillo (ARM) in an EGF signaling independent manner [60], demonstrating that there is crossover between these pathways independent of the outcome of EGF signaling. Third, EGF signaling has also been shown to link WNT signaling components with Toll signaling, by inducing expression of wntD, which limits dorsal (dl) nuclear localization at the poles and along the dorsal-ventral axis of the developing embryo [59]. Our recent findings further suggest that EGF signaling directly influences toll in embryonic patterning (unpublished observations). Overall, recent work on EGF signaling in non-neuroectodermal ventral patterning shows that EGF signaling is capable of playing a wide-ranging and diverse set of roles in its pathway interactions.

4. Specification of Muscle Precursors and Muscle Attachment to Tendons

Yarnitzky et al. showed in 1999 that vein (vn) and spitz (spi) are involved in the development of muscle precursors and argued that vn is qualitatively different from spi to avoid arg-mediated negative feedback into the EGF signaling process [64]. Unfortunately, since then few new insights have emerged into EGF’s function in developing muscle tissue in embryogenesis. The main recent findings: (1) the EGF pathway controls the specification and later maintenance of Adult Muscle Precursors [65]; and (2) that EGF signaling through vn and spi permits regenerative proliferation of multipoint gastric stem cells after damage [66]. Taken together, these findings both support the initial interpretation that vn differs qualitatively from spi in muscle precursor development [64]. More importantly, these findings provide significant evidence for an emerging theme in Drosophila EGF signaling: that most EGF mediated pathways in Drosophila embryogenesis are involved both in the initial establishment and subsequent maintenance of processes, as shown by the requirement for EGF in both repair and establishment of muscle tissue.
The multiple roles for EGF signaling necessitate the need for tight regulatory mechanisms ensuring the correct development of a complex organism, and at the same time, allowing flexibility in damage repair, and preventing aberrant growth. As many experiments in a variety of model organisms and in humans have shown, errors in EGF regulation result in highly tumorigenic growth, as the pathway’s excessive activation allows uncontrolled growth of many tissue types as well as metastasis [67].

5. Specification of Tracheal Invagination

A new focus for embryonic research is the effect of EGF signaling in tracheal development, specifically its role in specification of tissue integrity. EGF signaling was found to be necessary for maintaining tissue integrity of epithelial cells during tracheal development through modulation of cell adhesion a function independent of pointed one of the main transcription factors under the regulation of EGF [68]. Activation of EGF signaling leads to increased stiffness in epithelial tissue, and down-regulation of EGF signaling has the opposite effect [68], demonstrating a connection between EGF and Cadherin-modulated cell adhesion. The precise mechanism by which EGF modulates Cadherin based cell adhesion remains unclear, but a connection was suggested by another study which showed that in the absence of EGF signaling, developing tracheal cells are unable to concentrate filamentous Actin causing a defect in tracheal invagination [69]. EGF signaling was also shown to coordinate tracheal invagination by encircling the invagination site and promoting myosin cell intercalation [70]. The net result is a precise spatiotemporal activation sequence of EGF, which causes cells to invaginate as a group. Without EGF signaling, individual cells ingress without a clear coordinating strategy in the tissue, suggesting that EGF is crucial for specifying precise tissue boundaries in tracheal development [70,71]. Additionally, two receptor tyrosine phosphatases PTP10D and PTP4e negatively regulate EGF signaling during tracheal development [72]. Studies of tracheal development suggest that EGF provides very precise spatial and temporal information to cells in order to coordinate unified tissue development and movement.

6. Cell Recruitment to the Chordotonal Organs

Recent work has provided some additional insight into EGF’s influence on development of Chordotonal organs. Inbal et al. had previously shown that atonal (a transcription factor responsible for nerve cell development) triggers rhomboid expression, causing EGF activation, which initiates recruitment of attachment cells from the ectoderm and induce their specialization [73]. Again, it appears clear that EGF signaling is a common mechanism for precise and accurate tissue differentiation and maintenance of differentiation to prevent waste.

7. Specification of Oenocytes

In 2004, Brodu et al. [69] showed that oenocyte delamination occurs through discrete bursts, mediated by a localized EGF response. They found that EGF signaling triggered sequential activation of targets, which temporally communicated cell movement, a process which was continued by post-EGF mediated intracellular signaling [74]. Aside from this work, oenocyte specification has seen limited attention in the past 15 years.

8. Dorsal Midline Patterning (Including Dorsal Closure and Viability of Dorsal Midline Cells)

Antagonism between Dpp and EGF signaling is responsible for dorsal closure and corresponding patterning of cells near the dorsal midline [58]. Dpp blocks EGF signaling stimulating apoptosis near the midline [58]. This relationship is not unidirectional as EGF signaling down-regulates expression of Dpp in the epidermis, and prevents apoptosis in amnioserosa cells [75]. This signaling process highlights another common theme: EGF signaling is often required as the positive regulator of cell viability in key developmental processes, but also directly regulates the apoptosis gene Hid [76].

9. Other Signaling Pathways

EGF signaling is responsible for modulating square cell packing in the presumptive Drosophila pharynx by affecting planar cell polarity of cells in a square cell grid [77]. This process requires the effector Pins to orient mitotic spindles perpendicularly to the midline of the cell, which causes cells to adopt the square cell packed conformation [77]. This finding highlights the growing impact of EGF signaling on cell polarity.

10. Conclusions

10.1. Common Themes

(1)
EGF often functions in cell growth and proliferation and regulates apoptosis.
(2)
EGF signaling in Drosophila is highly self-regulating, particularly through RHO-1 and STAR mediated processing of EGF ligands and post-transcriptional control of ARGOS expression.
(3)
EGF signaling also can differ qualitatively due to expression of different yet functionally similar ligands (i.e., vein vs. spi). The availability of ligands with slightly different functionality allows EGF signaling to be precisely regulated based on ligand availability, rather than attempting to modulate the effects of the pathway after activation by a single ligand.
(4)
EGF signaling functions in cell adhesion and polarity. These four characteristics make EGF signaling highly effective at modulating tissue boundaries involving multiple alternative signaling pathways, as EGF signaling can self-regulate expression and interact with many other pathways through both negative and positive regulation.

10.2. Utility of Studying the EGF Signaling Pathway

Overall, EGF signaling is a crucial factor in promoting cell survival and differentiation. The study of EGF signaling in Drosophila embryogenesis has proven to be a valuable tool for understanding the complex mechanisms which give rise to the complexity of the various tissue systems of the Drosophila embryo. Careful study and analysis of EGF signaling mechanisms can reveal broader themes about embryonic control of cell fate, and are highly clinically applicable in understanding how aberrant EGF signaling can contribute to a wide range of human pathologies. This review did not cover other EGF pathways such as PI3K/PTEN, mTOR and Src as this has mainly been studied in larval and adult tissues. For EGF pathway studies, these tissues have provided a variety of useful tumor and drug screening models [78], aging models [79], colon stem cell models [80,81], brain cancer [82,83], and cell proliferation models [84].
With the advent of incredibly powerful new gene editing technologies such as the CRISPR/Cas9 system, and new mutants revealed in the genetic screens [85], there exists a plethora of new opportunities to study the pathway. New tools, such as live imaging in three dimensions and novel methods of fluorescent tagging, allow for the analysis of phenotypes which would otherwise be too ambiguous or complex to be studied through traditional means. In the WNT pathway, optogenetic approaches have been used to activate the co-receptor LRP6 (LDL receptor related protein, or Arrow in Drosophila) through a CRY2 fusion and blue light, an approach that should work for EGFR [76,86,87,88]. While much of the classic research on the pathway centered around polehole and the faint little ball phenotype, new methods will allow much more precise study of intracellular components of the pathway, and will allow a deeper understanding of the complex interactions between the EGF pathway and more well-studied embryonic signaling pathways, helping to further unravel the complexities of the developing Drosophila embryo. The work we have presented here represents a broad foundation upon which a deeper understanding of the mechanisms through which EGF signaling affects cancer can be built.

Acknowledgments

We wish to thank Mei-Mei Tan for her help in graphically designing Figure 1 for this review. This work was funded by grants from the Academic Research Fund MOE2014-T2-2-039 to Nicholas S. Tolwinski the Yale-NUS Bridging Grant IG15-B001 to Nicholas S. Tolwinski.

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Price, J.V.; Clifford, R.J.; Schupbach, T. The maternal ventralizing locus torpedo is allelic to faint little ball, an embryonic lethal, and encodes the Drosophila EGFR receptor homolog. Cell 1989, 56, 1085–1092. [Google Scholar] [CrossRef]
  2. Baker, N.E.; Rubin, G.M. Ellipse mutations in the Drosophila homologue of the EGFR receptor affect pattern formation, cell division, and cell death in eye imaginal discs. Dev. Biol. 1992, 150, 381–396. [Google Scholar] [CrossRef]
  3. Schejter, E.D.; Shilo, B.Z. The Drosophila EGFR receptor homolog (DER) gene is allelic to faint little ball, a locus essential for embryonic development. Cell 1989, 56, 1093–1104. [Google Scholar] [CrossRef]
  4. Clifford, R.; Schupbach, T. Molecular analysis of the Drosophila EGFR receptor homolog reveals that several genetically defined classes of alleles cluster in subdomains of the receptor protein. Genetics 1994, 137, 531–550. [Google Scholar] [PubMed]
  5. Clifford, R.J.; Schupbach, T. Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGFR receptor gene. Genetics 1989, 123, 771–787. [Google Scholar] [PubMed]
  6. Bogdan, S.; Klambt, C. Epidermal growth factor receptor signaling. Curr. Biol. 2001, 11, R292–R295. [Google Scholar] [CrossRef]
  7. Attrill, H.; Falls, K.; Goodman, J.L.; Millburn, G.H.; Antonazzo, G.; Rey, A.J.; Marygold, S.J.; FlyBase, C. Flybase: Establishing a gene group resource for Drosophila melanogaster. Nucleic Acids Res. 2016, 44, D786–D792. [Google Scholar] [CrossRef] [PubMed]
  8. UniProt, C. Uniprot: A hub for protein information. Nucleic Acids Res. 2015, 43, D204–D212. [Google Scholar]
  9. Klein, D.E.; Stayrook, S.E.; Shi, F.; Narayan, K.; Lemmon, M.A. Structural basis for EGFR ligand sequestration by argos. Nature 2008, 453, 1271–1275. [Google Scholar] [CrossRef] [PubMed]
  10. Austin, C.L.; Manivannan, S.N.; Simcox, A. TGF-alpha ligands can substitute for the neuregulin Vein in Drosophila development. Development 2014, 141, 4110–4114. [Google Scholar] [CrossRef] [PubMed]
  11. Steinhauer, J.; Liu, H.H.; Miller, E.; Treisman, J.E. Trafficking of the EGFR ligand Spitz regulates its signaling activity in polarized tissues. J. Cell Sci. 2013, 126, 4469–4478. [Google Scholar] [CrossRef] [PubMed]
  12. Paul, L.; Wang, S.H.; Manivannan, S.N.; Bonanno, L.; Lewis, S.; Austin, C.L.; Simcox, A. Dpp-induced EGFR signaling triggers postembryonic wing development in Drosophila. Proc. Natl. Acad. Sci. USA 2013, 110, 5058–5063. [Google Scholar] [CrossRef] [PubMed]
  13. Neuman-Silberberg, F.S.; Schüpbach, T. The Drosophila dorsoventral patterning gene gurken produces a dorsally localized rna and encodes a TGFα-like protein. Cell 1993, 75, 165–174. [Google Scholar] [CrossRef]
  14. Malartre, M. Regulatory mechanisms of EGFR signalling during Drosophila eye development. Cell. Mol. Life Sci. 2016, 73, 1825–1843. [Google Scholar] [CrossRef] [PubMed]
  15. Reich, A.; Shilo, B.Z. Keren, a new ligand of the Drosophila epidermal growth factor receptor, undergoes two modes of cleavage. EMBO J. 2002, 21, 4287–4296. [Google Scholar] [CrossRef] [PubMed]
  16. Urban, S.; Lee, J.R.; Freeman, M. A family of Rhomboid intramembrane proteases activates all Drosophila membrane-tethered EGFR ligands. EMBO J. 2002, 21, 4277–4286. [Google Scholar] [CrossRef] [PubMed]
  17. Lim, B.; Dsilva, C.J.; Levario, T.J.; Lu, H.; Schupbach, T.; Kevrekidis, I.G.; Shvartsman, S.Y. Dynamics of inductive erk signaling in the Drosophila embryo. Curr. Biol. 2015, 25, 1784–1790. [Google Scholar] [CrossRef] [PubMed]
  18. Schnepp, B.; Grumbling, G.; Donaldson, T.; Simcox, A. Vein is a novel component in the Drosophila epidermal growth factor receptor pathway with similarity to the neuregulins. Genes Dev. 1996, 10, 2302–2313. [Google Scholar] [CrossRef] [PubMed]
  19. Gabay, L.; Scholz, H.; Golembo, M.; Klaes, A.; Shilo, B.Z.; Klambt, C. EGF receptor signaling induces pointed p1 transcription and inactivates yan protein in the Drosophila embryonic ventral ectoderm. Development 1996, 122, 3355–3362. [Google Scholar] [PubMed]
  20. Shilo, B.Z. The regulation and functions of MAPK pathways in Drosophila. Methods 2014, 68, 151–159. [Google Scholar] [CrossRef] [PubMed]
  21. Urban, S.; Lee, J.R.; Freeman, M. Drosophila Rhomboid-1 defines a family of putative intramembrane serine proteases. Cell 2001, 107, 173–182. [Google Scholar] [CrossRef]
  22. Barberán, S.; Martín-Durán, J.M.; Cebrià, F. Evolution of the EGFR pathway in metazoa and its diversification in the planarian schmidtea mediterranea. Sci. Rep. 2016. [Google Scholar] [CrossRef] [PubMed]
  23. Lee, J.R.; Urban, S.; Garvey, C.F.; Freeman, M. Regulated intracellular ligand transport and proteolysis control EGFR signal activation in Drosophila. Cell 2001, 107, 161–171. [Google Scholar] [CrossRef]
  24. Tsruya, R.; Schlesinger, A.; Reich, A.; Gabay, L.; Sapir, A.; Shilo, B.Z. Intracellular trafficking by star regulates cleavage of the Drosophila EGFR receptor ligand Spitz. Genes Dev. 2002, 16, 222–234. [Google Scholar] [CrossRef] [PubMed]
  25. Shilo, B.Z. Developmental roles of Rhomboid proteases. Semin. Cell Dev. Biol. 2016, 60, 5–9. [Google Scholar] [CrossRef] [PubMed]
  26. Kolodkin, A.L.; Pickup, A.T.; Lin, D.M.; Goodman, C.S.; Banerjee, U. Characterization of star and its interactions with sevenless and EGFR receptor during photoreceptor cell development in Drosophila. Development 1994, 120, 1731–1745. [Google Scholar] [PubMed]
  27. Tsruya, R.; Wojtalla, A.; Carmon, S.; Yogev, S.; Reich, A.; Bibi, E.; Merdes, G.; Schejter, E.; Shilo, B.Z. Rhomboid cleaves star to regulate the levels of secreted Spitz. EMBO J. 2007, 26, 1211–1220. [Google Scholar] [CrossRef] [PubMed]
  28. Bang, A.G.; Kintner, C. Rhomboid and star facilitate presentation and processing of the Drosophila TGF-alpha homolog Spitz. Genes Dev. 2000, 14, 177–186. [Google Scholar] [PubMed]
  29. Bier, E.; Jan, L.Y.; Jan, Y.N. Rhomboid, a gene required for dorsoventral axis establishment and peripheral nervous system development in Drosophila melanogaster. Genes Dev. 1990, 4, 190–203. [Google Scholar] [CrossRef] [PubMed]
  30. Rebay, I.; Rubin, G.M. Yan functions as a general inhibitor of differentiation and is negatively regulated by activation of the Ras1/MAPK pathway. Cell 1995, 81, 857–866. [Google Scholar] [CrossRef]
  31. Maixner, A.; Hecker, T.P.; Phan, Q.N.; Wassarman, D.A. A screen for mutations that prevent lethality caused by expression of activated sevenless and Ras1 in the Drosophila embryo. Dev. Genet. 1998, 23, 347–361. [Google Scholar] [CrossRef]
  32. Egan, S.E.; Giddings, B.W.; Brooks, M.W.; Buday, L.; Sizeland, A.M.; Weinberg, R.A. Association of Sos Ras exchange protein with Grb2 is implicated in tyrosine kinase signal transduction and transformation. Nature 1993, 363, 45–51. [Google Scholar] [CrossRef] [PubMed]
  33. Kramer, S.; Okabe, M.; Hacohen, N.; Krasnow, M.A.; Hiromi, Y. Sprouty: A common antagonist of FGF and EGFR signaling pathways in Drosophila. Development 1999, 126, 2515–2525. [Google Scholar] [PubMed]
  34. Casci, T.; Vinos, J.; Freeman, M. Sprouty, an intracellular inhibitor of Ras signaling. Cell 1999, 96, 655–665. [Google Scholar] [CrossRef]
  35. Marais, R.; Light, Y.; Paterson, H.F.; Marshall, C.J. Ras recruits Raf-1 to the plasma membrane for activation by tyrosine phosphorylation. EMBO J. 1995, 14, 3136–3145. [Google Scholar] [PubMed]
  36. Stokoe, D.; Macdonald, S.; Cadwallader, K.; Symons, M.; Hancock, J. Activation of Raf as a result of recruitment to the plasma membrane. Science 1994, 264, 1463–1467. [Google Scholar] [CrossRef] [PubMed]
  37. Radke, K.; Johnson, K.; Guo, R.; Davidson, A.; Ambrosio, L. Drosophila-raf acts to elaborate dorsoventral pattern in the ectoderm of developing embryos. Genetics 2001, 159, 1031–1044. [Google Scholar] [PubMed]
  38. Tsuda, L.; Inoue, Y.H.; Yoo, M.A.; Mizuno, M.; Hata, M.; Lim, Y.M.; Adachi-Yamada, T.; Ryo, H.; Masamune, Y.; Nishida, Y. A protein kinase similar to MAP kinase activator acts downstream of the raf kinase in Drosophila. Cell 1993, 72, 407–414. [Google Scholar] [CrossRef]
  39. Gabay, L.; Seger, R.; Shilo, B.Z. In situ activation pattern of Drosophila EGFR receptor pathway during development. Science 1997, 277, 1103–1106. [Google Scholar] [CrossRef] [PubMed]
  40. Biggs, W.H., 3rd; Zavitz, K.H.; Dickson, B.; van der Straten, A.; Brunner, D.; Hafen, E.; Zipursky, S.L. The Drosophila rolled locus encodes a MAP kinase required in the sevenless signal transduction pathway. EMBO J. 1994, 13, 1628–1635. [Google Scholar] [PubMed]
  41. Lim, Y.M.; Tsuda, L.; Inoue, Y.H.; Irie, K.; Adachi-Yamada, T.; Hata, M.; Nishi, Y.; Matsumoto, K.; Nishida, Y. Dominant mutations of Drosophila MAP kinase kinase and their activities in Drosophila and yeast MAP kinase cascades. Genetics 1997, 146, 263–273. [Google Scholar] [PubMed]
  42. Oellers, N.; Hafen, E. Biochemical characterization of rolledsem, an activated form of Drosophila mitogen-activated protein kinase. J. Biol. Chem. 1996, 271, 24939–24944. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  43. Brunner, D.; Oellers, N.; Szabad, J.; Biggs, W.H., 3rd; Zipursky, S.L.; Hafen, E. A gain-of-function mutation in Drosophila MAP kinase activates multiple receptor tyrosine kinase signaling pathways. Cell 1994, 76, 875–888. [Google Scholar] [CrossRef]
  44. Scholz, H.; Deatrick, J.; Klaes, A.; Klambt, C. Genetic dissection of pointed, a Drosophila gene encoding two ETS-related proteins. Genetics 1993, 135, 455–468. [Google Scholar] [PubMed]
  45. Samakovlis, C.; Hacohen, N.; Manning, G.; Sutherland, D.C.; Guillemin, K.; Krasnow, M.A. Development of the Drosophila tracheal system occurs by a series of morphologically distinct but genetically coupled branching events. Development 1996, 122, 1395–1407. [Google Scholar] [PubMed]
  46. Von Ohlen, T.; Doe, C.Q. Convergence of dorsal, Dpp, and EGFR signaling pathways subdivides the Drosophila neuroectoderm into three dorsal-ventral columns. Dev. Biol. 2000, 224, 362–372. [Google Scholar] [CrossRef] [PubMed]
  47. Skeath, J.B.; Thor, S. Genetic control of Drosophila nerve cord development. Curr. Opin. Neurobiol. 2003, 13, 8–15. [Google Scholar] [CrossRef]
  48. Skeath, J.B. The Drosophila EGFR receptor controls the formation and specification of neuroblasts along the dorsal-ventral axis of the Drosophila embryo. Development 1998, 125, 3301–3312. [Google Scholar] [PubMed]
  49. Esteves, P.; Pecqueur, C.; Ransy, C.; Esnous, C.; Lenoir, V.; Bouillaud, F.; Bulteau, A.L.; Lombes, A.; Prip-Buus, C.; Ricquier, D.; et al. Mitochondrial retrograde signaling mediated by UCP2 inhibits cancer cell proliferation and tumorigenesis. Cancer Res. 2014, 74, 3971–3982. [Google Scholar] [CrossRef] [PubMed]
  50. Kim, I.O.; Kim, I.C.; Kim, S.; Kwon, Y.K.; Han, P.L.; Jeon, S.H.; Kim, S.H. Cns midline cells contribute to maintenance of the initial dorsoventral patterning of the Drosophila ventral neuroectoderm. J. Neurobiol. 2005, 62, 397–405. [Google Scholar] [CrossRef] [PubMed]
  51. Zhao, G.; Skeath, J.B. The Sox-domain containing gene Dichaete/fish-hook acts in concert with vnd and ind to regulate cell fate in the Drosophila neuroectoderm. Development 2002, 129, 1165–1174. [Google Scholar] [PubMed]
  52. Hong, J.W.; Park, K.W.; Levine, M.S. Temporal regulation of single-minded target genes in the ventral midline of the Drosophila central nervous system. Dev. Biol. 2013, 380, 335–343. [Google Scholar] [CrossRef] [PubMed]
  53. Zhao, G.; Wheeler, S.R.; Skeath, J.B. Genetic control of dorsoventral patterning and neuroblast specification in the Drosophila central nervous system. Int. J. Dev. Biol. 2007, 51, 107–115. [Google Scholar] [CrossRef] [PubMed]
  54. Kim, K.T.; Chiba, Y.; Arai, H.; Ishii, M. Discovery of an intermolecular disulfide bond required for the thermostability of a heterodimeric protein from the thermophile hydrogenobacter thermophilus. Biosci. Biotechnol. Biochem. 2016, 80, 232–240. [Google Scholar] [CrossRef] [PubMed]
  55. Kim, S.Y.; Jung, K.I.; Kim, S.H.; Jeon, S.H. Dpp represses eagle expression at short-range, but can repress its expression at a long-range via EGFR signal repression. Mol. Cells 2008, 26, 576–582. [Google Scholar] [PubMed]
  56. Moses, C.; Helman, A.; Paroush, Z.; von Ohlen, T. Phosphorylation of Ind by MAP kinase enhances Ind-dependent transcriptional repression. Dev. Biol. 2011, 360, 208–215. [Google Scholar] [CrossRef] [PubMed]
  57. Hwang, H.J.; Rulifson, E. Serial specification of diverse neuroblast identities from a neurogenic placode by notch and EGFR signaling. Development 2011, 138, 2883–2893. [Google Scholar] [CrossRef] [PubMed]
  58. Chang, T.; Shy, D.; Hartenstein, V. Antagonistic relationship between Dpp and EGFR signaling in Drosophila head patterning. Dev. Biol. 2003, 263, 103–113. [Google Scholar] [CrossRef]
  59. Helman, A.; Lim, B.; Andreu, M.J.; Kim, Y.; Shestkin, T.; Lu, H.; Jimenez, G.; Shvartsman, S.Y.; Paroush, Z. RTK signaling modulates the Dorsal gradient. Development 2012, 139, 3032–3039. [Google Scholar] [CrossRef] [PubMed]
  60. Hall, E.T.; Verheyen, E.M. Ras-activated Dsor1 promotes wnt signaling in Drosophila development. J. Cell Sci. 2015, 128, 4499–4511. [Google Scholar] [CrossRef] [PubMed]
  61. Urban, S.; Brown, G.; Freeman, M. EGF receptor signalling protects smooth-cuticle cells from apoptosis during Drosophila ventral epidermis development. Development 2004, 131, 1835–1845. [Google Scholar] [CrossRef] [PubMed]
  62. Payre, F.; Vincent, A.; Carreno, S. ovo/svb integrates Wingless and DER pathways to control epidermis differentiation. Nature 1999, 400, 271–275. [Google Scholar] [CrossRef] [PubMed]
  63. O’Keefe, L.; Dougan, S.T.; Gabay, L.; Raz, E.; Shilo, B.Z.; DiNardo, S. Spitz and Wingless, emanating from distinct borders, cooperate to establish cell fate across the engrailed domain in the Drosophila epidermis. Development 1997, 124, 4837–4845. [Google Scholar] [PubMed]
  64. Yarnitzky, T.; Min, L.; Volk, T. An interplay between two EGFR-receptor ligands, Vein and Spitz, is required for the formation of a subset of muscle precursors in Drosophila. Mech. Dev. 1998, 79, 73–82. [Google Scholar] [CrossRef]
  65. Figeac, N.; Jagla, T.; Aradhya, R.; da Ponte, J.P.; Jagla, K. Specification and behavior of AMPs, muscle-committed transient Drosophila stem cells. Fly 2011, 5, 7–9. [Google Scholar] [CrossRef] [PubMed]
  66. Wang, C.; Guo, X.; Xi, R. EGFR and notch signaling respectively regulate proliferative activity and multiple cell lineage differentiation of Drosophila gastric stem cells. Cell Res. 2014, 24, 610–627. [Google Scholar] [CrossRef] [PubMed]
  67. Lu, Z.; Jiang, G.; Blume-Jensen, P.; Hunter, T. Epidermal growth factor-induced tumor cell invasion and metastasis initiated by dephosphorylation and downregulation of focal adhesion kinase. Mol. Cell. Biol. 2001, 21, 4016–4031. [Google Scholar] [CrossRef] [PubMed]
  68. Cela, C.; Llimargas, M. EGFR is essential for maintaining epithelial integrity during tracheal remodelling in Drosophila. Development 2006, 133, 3115–3125. [Google Scholar] [CrossRef] [PubMed]
  69. Brodu, V.; Casanova, J. The RhoGAP crossveinless-c links trachealess and EGFR signaling to cell shape remodeling in Drosophila tracheal invagination. Genes Dev. 2006, 20, 1817–1828. [Google Scholar] [CrossRef] [PubMed]
  70. Nishimura, M.; Inoue, Y.; Hayashi, S. A wave of EGFR signaling determines cell alignment and intercalation in the Drosophila tracheal placode. Development 2007, 134, 4273–4282. [Google Scholar] [CrossRef] [PubMed]
  71. Letizia, A.; Sotillos, S.; Campuzano, S.; Llimargas, M. Regulated Crb accumulation controls apical constriction and invagination in Drosophila tracheal cells. J. Cell Sci. 2011, 124, 240–251. [Google Scholar] [CrossRef] [PubMed]
  72. Jeon, M.; Zinn, K. Receptor tyrosine phosphatases control tracheal tube geometries through negative regulation of EGFR signaling. Development 2009, 136, 3121–3129. [Google Scholar] [CrossRef] [PubMed]
  73. Inbal, A.; Volk, T.; Salzberg, A. Recruitment of ectodermal attachment cells via an EGFR-dependent mechanism during the organogenesis of Drosophila proprioceptors. Dev. Cell 2004, 7, 241–250. [Google Scholar] [CrossRef] [PubMed]
  74. Brodu, V.; Elstob, P.R.; Gould, A.P. EGF receptor signaling regulates pulses of cell delamination from the Drosophila ectoderm. Dev. Cell 2004, 7, 885–895. [Google Scholar] [CrossRef] [PubMed]
  75. Shen, W.; Chen, X.; Cormier, O.; Cheng, D.C.; Reed, B.; Harden, N. Modulation of morphogenesis by EGFR during dorsal closure in Drosophila. PLoS ONE 2013, 8, e60180. [Google Scholar] [CrossRef] [PubMed]
  76. Bergmann, A.; Agapite, J.; McCall, K.; Steller, H. The Drosophila gene hid is a direct molecular target of Ras-dependent survival signaling. Cell 1998, 95, 331–341. [Google Scholar] [CrossRef]
  77. Tamada, M.; Zallen, J.A. Square cell packing in the Drosophila embryo through spatiotemporally regulated EGFR receptor signaling. Dev. Cell 2015, 35, 151–161. [Google Scholar] [CrossRef] [PubMed]
  78. Levine, B.D.; Cagan, R.L. Drosophila lung cancer models identify trametinib plus statin as candidate therapeutic. Cell Rep. 2016, 14, 1477–1487. [Google Scholar] [CrossRef] [PubMed]
  79. Gruber, J.; Yee, Z.; Tolwinski, N.S. Developmental drift and the role of wnt signaling in aging. Cancers 2016, 8, 73. [Google Scholar] [CrossRef] [PubMed]
  80. Jiang, H.; Edgar, B.A. EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors. Development 2009, 136, 483–493. [Google Scholar] [CrossRef] [PubMed]
  81. Buchon, N.; Broderick, N.A.; Kuraishi, T.; Lemaitre, B. Drosophila EGFR pathway coordinates stem cell proliferation and gut remodeling following infection. BMC Biol. 2010. [Google Scholar] [CrossRef] [PubMed]
  82. Read, R.D. Drosophila melanogaster as a model system for human brain cancers. Glia 2011, 59, 1364–1376. [Google Scholar] [CrossRef] [PubMed]
  83. Read, R.D.; Cavenee, W.K.; Furnari, F.B.; Thomas, J.B. A Drosophila model for EGFR-Ras and PI3K-dependent human glioma. PLoS Genet. 2009, 5, e1000374. [Google Scholar] [CrossRef] [PubMed]
  84. Sonoshita, M.; Cagan, R.L. Modeling human cancers in Drosophila. Curr. Top. Dev. Biol. 2017, 121, 287–309. [Google Scholar] [PubMed]
  85. Yamamoto, S.; Jaiswal, M.; Charng, W.L.; Gambin, T.; Karaca, E.; Mirzaa, G.; Wiszniewski, W.; Sandoval, H.; Haelterman, N.A.; Xiong, B.; et al. A Drosophila genetic resource of mutants to study mechanisms underlying human genetic diseases. Cell 2014, 159, 200–214. [Google Scholar] [CrossRef] [PubMed]
  86. Bugaj, L.J.; Spelke, D.P.; Mesuda, C.K.; Varedi, M.; Kane, R.S.; Schaffer, D.V. Regulation of endogenous transmembrane receptors through optogenetic Cry2 clustering. Nat. Commun. 2015. [Google Scholar] [CrossRef] [PubMed]
  87. Tischer, D.; Weiner, O.D. Illuminating cell signalling with optogenetic tools. Nat. Rev. Mol. Cell Biol. 2014, 15, 551–558. [Google Scholar] [CrossRef] [PubMed]
  88. Johnson, H.E.; Goyal, Y.; Pannucci, N.L.; Schupbach, T.; Shvartsman, S.Y.; Toettcher, J.E. The spatiotemporal limits of developmental erk signaling. Dev. Cell 2017, 40, 185–192. [Google Scholar] [CrossRef] [PubMed]
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Figure 1. Diagrammatic representation of the EGF signaling pathway. Question marks refer to the still unknown mechanism of Sprouty action.
Figure 1. Diagrammatic representation of the EGF signaling pathway. Question marks refer to the still unknown mechanism of Sprouty action.
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Figure 2. The signaling pathways involved in dorsal-ventral patterning of the neuroectoderm as shown by a transverse section of an early stage embryo (Stage 5 just before gastrulation starts).
Figure 2. The signaling pathways involved in dorsal-ventral patterning of the neuroectoderm as shown by a transverse section of an early stage embryo (Stage 5 just before gastrulation starts).
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Lusk, J.B.; Lam, V.Y.M.; Tolwinski, N.S. Epidermal Growth Factor Pathway Signaling in Drosophila Embryogenesis: Tools for Understanding Cancer. Cancers 2017, 9, 16. https://doi.org/10.3390/cancers9020016

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Lusk JB, Lam VYM, Tolwinski NS. Epidermal Growth Factor Pathway Signaling in Drosophila Embryogenesis: Tools for Understanding Cancer. Cancers. 2017; 9(2):16. https://doi.org/10.3390/cancers9020016

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Lusk, Jay B., Vanessa Y. M. Lam, and Nicholas S. Tolwinski. 2017. "Epidermal Growth Factor Pathway Signaling in Drosophila Embryogenesis: Tools for Understanding Cancer" Cancers 9, no. 2: 16. https://doi.org/10.3390/cancers9020016

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