Molecular Dissection of the 5S Ribosomal RNA-Intergenic Transcribed Spacers in Saccharum spp. and Tripidium spp.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. DNA Extraction and Quantification
2.3. PCR Amplification, Agarose Gel, and CE Analyses
2.4. Data Analysis
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genus | Species | Accession | Total | Gel Banding Type |
---|---|---|---|---|
Tripidium | arundinaceum | IS76-218, MPTH97-194, MPTH98-283, MPTH98-326 | 4 | IV |
bengalense | IMP9751 | 1 | IV | |
procerum | Kalimpong | 1 | V | |
Saccharum | officinarum | Akoki24 *, Badila Fiji *, Badila Java *, Badila, Bandjermasin Hitam *, Big Ribbon *, EK02 *, Fiji1, Fiji24 *, Fiji47, Green German*, Haw Orig 36 *, IJ76-418B *, IJ76-521 *, IM76-245 *, IN84-024 *, IN84-68A, Louisiana Purple*, Mentor4745, Muntok Java, NG21-003 *, NG21-017 *, NG57-223 *, NG77-066 *, NG77-142 *, NG77-241 *, NG96-024 *, NH70-069 *, Oi Deng, Saipan * | 30 | I, II |
spontaneum | Coimbatore, Dacca *, Djatiroto, Glagah *, GuangXi86-5, GuangXi87-21, GuangXi87-22, IMP9068, IMP9089, IN76-086 *, IN84-010 *, IN84-033 *, IN84-089 *, IN84-21, IND81-043 *, IND81-101 *, IND81-144, IND81-155 *, IND81-161, IND81-80, IND82-257A, IND82-311, IS76-121 *, Isiolo *, JW570, JW599, M. Moentai *, MOL1032A, MOL1032B, MPTH97-003, MPTH97-113, MPTH97-200, MPTH97-204, MPTH97-209, MPTH97-213, MPTH97-216, MPTH97-218, MPTH97-233, MPTH97-461, MPTH98-388, MPTH99-476, NG77-169 *, Okinawa #01 *, PCANOR84-2A, PCAV84-12A, PCAV84-12B, PCAV84-12C, PIN84-1B, PO84-3, PPGN84-08 *, S001 *, S66-121A, S66-84A, S66-84B, SES006, SES114, SES147B, SES189, SES205A, SES231, SES297B *, SES323A, SES84-58, S spont Pakistan *, Saudi Arabia *, SM7916 *, Tainan, US56-013-07 *, US56-15-1, US56-15-8, US57-141-05 * | 71 | I, II | |
robustum | IJ76-339 *, IJ76-534 *, IM76-232 *, IN84-045 *, IN84-076 *, M3035/66 *, Molokai 5573, NG28-289 *, NG57-054, NG57-055 *, NG77-021 *, NG77-084 *, NG77-147 *, NG77-159 *, NG77-235 *, NH70-015 *, Teboe SalakToewa * | 17 | I, II, III | |
barberi | Agoule *, Chin, Chunnee *, Dark Pindaria *, Dhaula, Ganapathy, Hatuni, Hemja *, Hullu Kabbu *, Kalari, Katha, Khagzi, Manga sic *, Nargori *, Panura, Pathri *, Paunra *, Rena, Rhea Sport, Rounda *, Ruckri *, Semari *, Sunnabile *, Tereru *, White Pararia * | 25 | I, II | |
sinense | Chuk Che *, Khakai *, Lu Cane *, Mcikum, Merthi Zell, Mia Lan *, Nepal 3 *, Tanzhon bamboo *, Tekcha Okinawa *, Tukuyu 1, Uba Del Natal *, Uba India *, Uba Naquin * | 13 | I, II | |
edule | IJ76-375 *, NG77-010 * | 2 | I | |
spp. hybrids | Ho01-564, HoCP00-950, HoCP04-838, HoCP96-540, LCP85-384 | 5 | III | |
Unknown | US4515-9200, SPS-269 | 2 | II |
Genus | Saccharum | Tripidium | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Species | barberi | edule | hybrid | officinarum | robustum | sinense | spontaneum | arundinaceum | bengalense | procerum | unknown |
No. Accessions | 25 | 2 | 5 | 30 | 17 | 13 | 71 | 4 | 1 | 1 | 2 |
Amplicons (bp) | Percent of each amplicon per species | ||||||||||
61 | 0 | 0 | 100 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
224–225 | 0 | 0 | 0 | 0 | 0 | 0 | 11.3 | 0 | 0 | 0 | 0 |
227 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 | 0 |
228–229 | 0 | 0 | 0 | 0 | 5.9 | 0 | 7 | 0 | 0 | 0 | 0 |
230–231 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 0 | 0 | 0 | 0 |
233 | 0 | 0 | 0 | 0 | 2.8 | 0 | 5.6 | 0 | 0 | 0 | 0 |
234 | 100 | 100 | 100 | 100 | 100 | 100 | 73.2 | 25 † | 100 | 0 | 100 |
235 | 100 | 100 | 100 | 100 | 100 | 100 | 94.4 | 25.0 † | 100 | 0 | 100 |
236 | 100 | 100 | 100 | 100 | 82.4 | 100 | 91.5 | 25.0 † | 100 | 0 | 100 |
237 | 36 | 100 | 60 | 36.7 | 17.6 | 84.6 | 42.3 | 0 | 0 | 0 | 0 |
238 | 0 | 0 | 0 | 3.3 | 8 | 0 | 2.8 | 0 | 0 | 0 | 0 |
247–248 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 |
382–385 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 † | 50 | 0 | 0 | 0 |
387, 388, 402 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 † | 0 | 0 | 0 | 0 |
403–405 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 † | 0 | 0 | 100 | 0 |
406 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
407 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 † | 50 | 0 | 0 | 0 |
408–409 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 † | 0 | 0 | 0 | 0 |
463–466 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100 | 0 |
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Pan, Y.-B.; Todd, J.R.; Lomax, L.; White, P.M., Jr.; Simpson, S.A.; Scheffler, B.E. Molecular Dissection of the 5S Ribosomal RNA-Intergenic Transcribed Spacers in Saccharum spp. and Tripidium spp. Agronomy 2023, 13, 2728. https://doi.org/10.3390/agronomy13112728
Pan Y-B, Todd JR, Lomax L, White PM Jr., Simpson SA, Scheffler BE. Molecular Dissection of the 5S Ribosomal RNA-Intergenic Transcribed Spacers in Saccharum spp. and Tripidium spp. Agronomy. 2023; 13(11):2728. https://doi.org/10.3390/agronomy13112728
Chicago/Turabian StylePan, Yong-Bao, James R. Todd, Lionel Lomax, Paul M. White, Jr., Sheron A. Simpson, and Brian E. Scheffler. 2023. "Molecular Dissection of the 5S Ribosomal RNA-Intergenic Transcribed Spacers in Saccharum spp. and Tripidium spp." Agronomy 13, no. 11: 2728. https://doi.org/10.3390/agronomy13112728