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Antibodies 2013, 2(1), 1-8; doi:10.3390/antib2010001

Generation of a Monoclonal Antibody Specifically Reacting with Neuron-specific TATA-Box Binding Protein-Associated Factor 1 (N-TAF1)

1
Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, 520-2192, Japan
2
Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, 259-1193, Japan
3
Advanced Molecular Epidemiology Research Institute, Faculty of Medicine, Yamagata University, Yamagata, 990-9585, Japan
*
Authors to whom correspondence should be addressed.
Received: 9 November 2012 / Revised: 10 December 2012 / Accepted: 14 December 2012 / Published: 21 December 2012
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Abstract

TATA-box binding protein-associated factor 1 (TAF1), the largest subunit of the transcription factor IID complex, plays an important role in the RNA polymerase II-mediated gene transcription pathway regulating the transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1) may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. The present study reports the preparation and properties of a monoclonal antibody directed against N-TAF1. The monoclonal antibody, 3A-11F, specifically recognized N-TAF1 protein with no reactivity to TAF1 protein, as evidenced by immunocytochemistry and immunoprecipitation using cultured cells expressing recombinant N-TAF1 or TAF1 protein. Immunohistochemistry using 3A-11F showed that N-TAF1-imunoreactivity was detected in the nuclear region of neurons in the rat brain. The 3A-11F monoclonal antibody promises to be a useful tool for determining the expression pattern and biological function of N-TAF1 in the brain. View Full-Text
Keywords: TAF1; transcription factor; immunohistochemistry; immunoprecipitation TAF1; transcription factor; immunohistochemistry; immunoprecipitation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Makino, S.; Masuda, C.; Ando, S.; Tamiya, G.; Tooyama, I. Generation of a Monoclonal Antibody Specifically Reacting with Neuron-specific TATA-Box Binding Protein-Associated Factor 1 (N-TAF1). Antibodies 2013, 2, 1-8.

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