Refolding Technologies for Antibody Fragments
AbstractRefolding is one of the production technologies for pharmaceutical grade antibody fragments. Detergents and denaturants are primarily used to solubilize the insoluble proteins. The solubilized and denatured proteins are refolded by reducing the concentration of the denaturants or detergents. Several refolding technologies have been used for antibody fragments, comprising dilution, dialysis, solid phase solvent exchange and size exclusion chromatography, as reviewed here. Aggregation suppressor or folding-assisting agents, including arginine hydrochloride, ionic liquids and detergents or denaturants at low concentrations, are included in the refolding solvent to enhance refolding yield. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Arakawa, T.; Ejima, D. Refolding Technologies for Antibody Fragments. Antibodies 2014, 3, 232-241.
Arakawa T, Ejima D. Refolding Technologies for Antibody Fragments. Antibodies. 2014; 3(2):232-241.Chicago/Turabian Style
Arakawa, Tsutomu; Ejima, Daisuke. 2014. "Refolding Technologies for Antibody Fragments." Antibodies 3, no. 2: 232-241.