Structural Changes in Stx1 Engineering Monoclonal Antibody Improves Its Functionality as Diagnostic Tool for a Rapid Latex Agglutination Test
Abstract
:1. Introduction
2. Materials and Methods
2.1. scFvStx1(I) Gene Design, Expression and Purification
2.2. scFvStx1(I) Gene Modifications
2.3. scFvStx1 Fragment Procurement
2.4. Rapid Latex Agglutination Test (RALT)
3. Results
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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scFvStx1(II) | IgG mAb | |
---|---|---|
Affinity constant (KD) | 2.26 × 10−7 M | 2.5 × 10−10 M |
Expression system | Bacterial | Hybridoma |
Yield after purification | 2 mg/L | 2 mg/L |
Time to obtain (weeks) | 1 | 5–7 |
Cross-reactivity to Stx2 * | Yes | Yes |
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Luz, D.; Shiga, E.A.; Chen, G.; Quintilio, W.; Andrade, F.B.; Maranhão, A.Q.; Caetano, B.A.; Mitsunari, T.; Silva, M.A.; Rocha, L.B.; et al. Structural Changes in Stx1 Engineering Monoclonal Antibody Improves Its Functionality as Diagnostic Tool for a Rapid Latex Agglutination Test. Antibodies 2018, 7, 9. https://doi.org/10.3390/antib7010009
Luz D, Shiga EA, Chen G, Quintilio W, Andrade FB, Maranhão AQ, Caetano BA, Mitsunari T, Silva MA, Rocha LB, et al. Structural Changes in Stx1 Engineering Monoclonal Antibody Improves Its Functionality as Diagnostic Tool for a Rapid Latex Agglutination Test. Antibodies. 2018; 7(1):9. https://doi.org/10.3390/antib7010009
Chicago/Turabian StyleLuz, Daniela, Emerson A. Shiga, Gang Chen, Wagner Quintilio, Fernanda B. Andrade, Andrea Q. Maranhão, Bruna A. Caetano, Thaís Mitsunari, Míriam A. Silva, Letícia B. Rocha, and et al. 2018. "Structural Changes in Stx1 Engineering Monoclonal Antibody Improves Its Functionality as Diagnostic Tool for a Rapid Latex Agglutination Test" Antibodies 7, no. 1: 9. https://doi.org/10.3390/antib7010009