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Pathogens 2017, 6(2), 20; doi:10.3390/pathogens6020020

Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm

1
Department of Clinical Medicine, Health, Aarhus University, DK-8200 Aarhus, Denmark
2
Department of Clinical Microbiology, Aarhus University Hospital, DK-8200 Aarhus, Denmark
3
Interdisciplinary Nanoscience Center, Aarhus University, DK-8000 Aarhus, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Lawrence S. Young
Received: 7 April 2017 / Revised: 12 May 2017 / Accepted: 14 May 2017 / Published: 19 May 2017
View Full-Text   |   Download PDF [568 KB, uploaded 19 May 2017]   |  

Abstract

Bacteria of genus Achromobacter are emerging pathogens in cystic fibrosis (CF) capable of biofilm formation and development of antimicrobial resistance. Evolutionary adaptions in the transition from primary to chronic infection were assessed by transcriptomic analysis of successive isolates of Achromobacter xylosoxidans from a single CF patient. Several efflux pump systems targeting antimicrobial agents were upregulated during the course of the disease, whereas all genes related to motility were downregulated. Genes annotated to subsystems of sulfur metabolism, protein metabolism and potassium metabolism exhibited the strongest upregulation. K+ channel genes were hyperexpressed, and a putative sulfite oxidase was more than 1500 times upregulated. The transcriptome patterns indicated a pivotal role of sulfur metabolism and electrical signalling in Achromobacter biofilms during late stage CF lung disease. View Full-Text
Keywords: cystic fibrosis; adaptation; antimicrobial resistance; efflux pumps; sulfur metabolism; transcriptomics cystic fibrosis; adaptation; antimicrobial resistance; efflux pumps; sulfur metabolism; transcriptomics
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MDPI and ACS Style

Nielsen, S.M.; Meyer, R.L.; Nørskov-Lauritsen, N. Differences in Gene Expression Profiles between Early and Late Isolates in Monospecies Achromobacter Biofilm. Pathogens 2017, 6, 20.

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