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Article
Peer-Review Record

A Questionnaire Integrated with the Digital Medical Record Improved the Coverage of a Control Program for Congenital Chagas Disease in Tuscany, Italy

Microorganisms 2023, 11(1), 154; https://doi.org/10.3390/microorganisms11010154
by Anna Barbiero 1, Martina Mazzi 2, Antonia Mantella 1,3, Michele Trotta 4,5, Gian Maria Rossolini 1,6, Alberto Antonelli 1, Patrizia Bordonaro 7, Maria Grazia Colao 6, Anna Rosa Speciale 8, Tullio Di Benedetto 8, Mariarosaria Di Tommaso 8, Elisabetta Mantengoli 4, Felice Petraglia 9, Luisa Galli 10, Marco Pezzati 11, Carlo Dani 12, Maria José Caldes Pinilla 13, Cecilia Berni 14, Bassam Dannaoui 15, Pedro Albajar Viñas 16, Alessandro Bartoloni 1,3,4 and Lorenzo Zammarchi 1,3,4,5,*add Show full author list remove Hide full author list
Reviewer 1:
Reviewer 2: Anonymous
Microorganisms 2023, 11(1), 154; https://doi.org/10.3390/microorganisms11010154
Submission received: 14 December 2022 / Revised: 31 December 2022 / Accepted: 2 January 2023 / Published: 6 January 2023
(This article belongs to the Special Issue 10th Anniversary of Microorganisms: Past, Present and Future)

Round 1

Reviewer 1 Report

The aim of this work is important given the lack of accuracy in the diagnosis of Chagas disease (In LATAM and Europe) and, above all, the morbidity rates that occur in patients. The relevance of screening in European countries in pregnant women highlights the importance of this disease, in addition to avoiding fatal outcomes in children who have been infected from the mother.

In line 59, change "continental Latin America" ​​to Latin American countries.

Question:

1) Why didn't you consider the use of q-PCR in peripheral blood of pregnant women?

2) Thinkin about the biology of T. cruzi, it is known that depending on the region there is a different distribution of DTUs, there are reports that indicate that a serological test can work in a country according to the T. cruzi DTU´s used for the serological test but in other countries it does not have the same effectiveness.
What was your criteria for the selection of the serological tests used for tha diagnosis?
Do you think there were false positive results?

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

Comments and suggestions for the authors are included in the attached word file. 

Comments for author File: Comments.pdf

Author Response

Please see the attachment

Author Response File: Author Response.docx

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