Lipid Production of Schizochytrium sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain Isolation and Identification
2.2. Growth Curve and Lipid Production of S. sp. HBW10
2.3. Lipid Production with Varied Carbon and Nitrogen Substrates
2.4. Batch Axenic Culture with Food Waste Hydrolysate (FWH)
2.4.1. Preparation of Food Waste Hydrolysate (FWH)
2.4.2. Lipid Production with Different Content Levels of FWH
2.4.3. Influence of Initial pH on Lipid Production
2.5. Analytical Methods
2.5.1. Biomass Analysis
2.5.2. Lipid extraction and analysis
3. Results
3.1. Strain Isolation and Phylogenetic Analysis
3.2. Characteristics of S. sp. HBW10 for Biomass and Lipid Accumulation
3.3. Lipid Production of S. sp. HBW10 under Varied Carbon and Nitrogen Substrates
3.4. Utilization of FWH as Feedstock for Lipid Production of S. sp. HBW10
3.5. Influence of the Initial pH on the Biomass and Lipid Production of S. sp. HBW10
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Cultivation Time (day) | FWH (%, v/v) | TFAs (g/L) | SFAs (%TFAs) | MUFAs (%TFAs) | PUFAs (%TFAs) |
---|---|---|---|---|---|
Initial pH adjustment of FWH1 medium by NaOH (FWH1-NaOH) | |||||
1 | 0 | 0.11 ± 0.02 | 57.36 ± 1.39 | 11.24 ± 1.05 | 20.01 ± 1.68 |
1 | 50 | 0.06 ± 0.01 | 45.90 ± 5.69 | 17.06 ± 3.18 | 8.32 ± 1.16 |
1 | 100 | 0.04 ± 0.00 | 55.26 ± 3.83 | 17.6 ± 1.09 | 3.43 ± 0.64 |
3 | 0 | 0.16 ± 0.04 | 47.28 ± 0.08 | 25.93 ± 0.85 | 19.61 ± 0.55 |
3 | 50 | 0.06 ± 0.02 | 43.68 ± 3.32 | 13.8 ± 0.29 | 5.20 ± 0.91 |
3 | 100 | 0.05 ± 0.00 | 45.91 ± 5.85 | 15.36 ± 5.51 | 7.65 ± 1.87 |
5 | 0 | 0.17 ± 0.01 | 46.5 ± 1.39 | 24.35 ± 3.04 | 22.02 ± 4.14 |
5 | 50 | 0.10 ± 0.00 | 34.07 ± 1.56 | 38.82 ± 0.16 | 0.85 ± 0.17 |
5 | 100 | 0.04 ± 0.00 | 54.80 ± 2.73 | 17.97 ± 1.10 | 1.30 ± 0.11 |
Initial pH adjustment of FWH2 medium by NaOH (FWH2-NaOH) | |||||
5 | 0 | 0.17 ± 0.01 | 46.50 ± 1.39 | 24.35 ± 3.04 | 22.02 ± 4.14 |
5 | 50 | 0.34 ± 0.03 | 34.61 ± 0.67 | 24.72 ± 0.92 | 27.81 ± 0.08 |
5 | 100 | 0.08 ± 0.01 | 28.06 ± 1.43 | 24.69 ± 1.28 | 0.92 ± 0.07 |
Initial pH adjustment of FWH1 medium by NaHCO3 (FWH1-NaHCO3) | |||||
5 | 0 | 0.17 ± 0.01 | 46.50 ± 1.39 | 24.35 ± 3.04 | 22.02 ± 4.14 |
5 | 50 | 0.20 ± 0.02 | 24.14 ± 2.64 | 24.38 ± 1.05 | 0.91 ± 0.26 |
5 | 100 | 0.11 ± 0.01 | 23.7 ± 1.80 | 26.15 ± 2.30 | 0.91 ± 0.25 |
Food Waste Hydrolysate (%, v/v) | pH | Biomass (g/L) | TFAs (g/L) | SFAs (%TFAs) | MUFAs (%TFAs) | PUFAs (%TFAs) |
---|---|---|---|---|---|---|
0 | 6.2 | 4.06 ± 0.27 | 0.17 ± 0.01 | 46.50 ± 1.39 | 24.35 ± 3.04 | 22.02 ± 4.14 |
0 | 7 | 4.07 ± 0.28 | 0.10 ± 0.01 | 65.23 ± 5.34 | 5.30 ± 0.83 | 29.48 ± 5.46 |
0 | 8 | 4.09 ± 0.09 | 0.17 ± 0.01 | 72.07 ± 4.02 | 4.55 ± 0.16 | 23.39 ± 3.95 |
50 | 6.2 | 0.77 ± 0.14 | 0.10 ± 0.02 | 34.07 ± 0.31 | 38.82 ± 1.41 | 0.85 ± 0.02 |
50 | 7 | 3.45 ± 0.0.54 | 0.11 ± 0.01 | 88.25 ± 3.14 | 11.75 ± 3.14 | 0.00 ± 0.00 |
50 | 8 | 3.80 ± 0.48 | 0.12 ± 0.01 | 89.84 ± 3.60 | 9.31 ± 2.23 | 0.85 ± 0.00 |
100 | 6.4 | 1.48 ± 0.59 | 0.04 ± 0.00 | 54.80 ± 0.40 | 17.97 ± 0.40 | 1.30 ± 0.00 |
100 | 7 | 1.92 ± 0.22 | 0.04 ± 0.00 | 97.70 ± 2.07 | 0.00 ± 0.00 | 2.30 ± 0.07 |
100 | 8 | 2.50 ± 0.18 | 0.05 ± 0.01 | 98.61 ± 1.96 | 0.00 ± 0.00 | 1.39 ± 0.06 |
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Li, X.; Yu, X.; Liu, Q.; Zhang, Y.; Wang, Q. Lipid Production of Schizochytrium sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate. Microorganisms 2023, 11, 2714. https://doi.org/10.3390/microorganisms11112714
Li X, Yu X, Liu Q, Zhang Y, Wang Q. Lipid Production of Schizochytrium sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate. Microorganisms. 2023; 11(11):2714. https://doi.org/10.3390/microorganisms11112714
Chicago/Turabian StyleLi, Xiaofang, Xinping Yu, Qian Liu, Yong Zhang, and Qiuzhen Wang. 2023. "Lipid Production of Schizochytrium sp. HBW10 Isolated from Coastal Waters of Northern China Cultivated in Food Waste Hydrolysate" Microorganisms 11, no. 11: 2714. https://doi.org/10.3390/microorganisms11112714