Next Article in Journal
Can Acanthamoeba Harbor Monkeypox Virus?
Next Article in Special Issue
Microbiological Characterization of the Biofilms Colonizing Bioplastics in Natural Marine Conditions: A Comparison between PHBV and PLA
Previous Article in Journal
HIV/Mtb Co-Infection: From the Amplification of Disease Pathogenesis to an “Emerging Syndemic”
Previous Article in Special Issue
Metagenomes from Coastal Sediments of Kuwait: Insights into the Microbiome, Metabolic Functions and Resistome
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50

1
College of Rural Revitalization, Jiangsu Open University, Nanjing 210036, China
2
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China
3
Suzhou Kaisiling Environmental Sci-Technology Co., Ltd., Suzhou 215413, China
*
Author to whom correspondence should be addressed.
Microorganisms 2023, 11(4), 854; https://doi.org/10.3390/microorganisms11040854
Submission received: 4 January 2023 / Revised: 24 March 2023 / Accepted: 24 March 2023 / Published: 27 March 2023
(This article belongs to the Collection Biodegradation and Environmental Microbiomes)

Abstract

:
Picolinic acid (PA) is a typical mono-carboxylated pyridine derivative produced by human/animals or microorganisms which could be served as nutrients for bacteria. Most Bordetella strains are pathogens causing pertussis or respiratory disease in humans and/or various animals. Previous studies indicated that Bordetella strains harbor the PA degradation pic gene cluster. However, the degradation of PA by Bordetella strains remains unknown. In this study, a reference strain of genus Bordetella, B. bronchiseptica RB50, was investigated. The organization of pic gene cluster of strain RB50 was found to be similar with that of Alcaligenes faecalis, in which the sequence similarities of each Pic proteins are between 60% to 80% except for PicB2 (47% similarity). The 3,6-dihydroxypicolinic acid (3,6DHPA) decarboxylase gene (BB0271, designated as picCRB50) of strain RB50 was synthesized and over-expressed in E. coli BL21(DE3). The PicCRB50 showed 75% amino acid similarities against known PicC from Alcaligenes faecalis. The purified PicCRB50 can efficiently transform 3,6DHPA to 2,5-dihydroxypyridine. The PicCRB50 exhibits optimal activities at pH 7.0, 35 °C, and the Km and kcat values of PicCRB50 for 3,6DHPA were 20.41 ± 2.60 μM and 7.61 ± 0.53 S−1, respectively. The present study provided new insights into the biodegradation of PA by pathogens of Bordetella spp.

1. Introduction

Pyridine derivatives are ubiquitous in natural environments and living bodies on the earth. Pyridine derivatives, such as nicotinic acid, PLP, and NAD(P)H, play important roles in organisms that are building blocks of organs, vitamins, or cofactors of enzymatic reactions [1]. However, some pyridine derivatives produced by one organism are toxic to another one. For example, nicotine is a natural product of tobacco plant which is toxic to pests and can be used as a pesticide [2]. These pyridine derivatives compounds are widely distributed in soil, water, and sediment. Microorganisms could decompose and use them as sole carbon or nitrogen sources, and in the meantime, reduce the concentrations of those toxic compounds [1,2,3,4,5].
Picolinic acid (PA) is a typical mono-carboxylated pyridine derivative produced by humans/animals or microorganisms [6,7,8]. PA is an isomer of nicotinic acid. Different from nicotinic acid with a C3-carboxyl group, the carboxyl group of PA is located at the C-2 position on the pyridine ring. The N atom of PA and the O atom of carboxyl-group form a chelating structure and lead it toxic to microorganisms [9,10,11]. Several bacteria have evolved the ability to degrade PA and utilize it as sole carbon or nitrogen sources for cell growth, including Gram-positive bacteria (Arthrobacter, Streptomyces, and Rhodococcus) and Gram-negative bacteria (Alcaligenes, Burkholderia, and Comamonas) [12,13,14,15,16,17]. The PA degradation pathway and pic gene cluster responsible for PA catabolism have been studied in Gram-negative strain Alcaligenes faecalis JQ135 (Figure 1) [12,13]. The upper pathway contains intermediates 6-hydroxypicolinic acid (6HPA) and 3,6-dihydroxypicolinic acid (3,6DHPA), which are catalyzed by PA dehydrogenase (PicA) and 6HPA monooxygenase (PicB). The lower pathway was 2,5-dihydroxypyridine (2,5DHP) to fumaric acid (a Krebs cycle intermediate), which was catalyzes by four conserved enzymes, 2,5DHP 5,6-dioxygenase (PicD), N-formylmaleamic acid deformylase (PicE), maleamic acid amidohydrolase (PicF), and maleic acid isomerase (PicG). The pic gene cluster was predicted as widely distributed in α-, β-, and γ-Proteobacteria [13,18].
Bordetella spp. Is a large group of microorganisms, most of which cause pertussis or respiratory disease in humans and/or various animals [19,20,21]. Our previous study indicated that the pic gene cluster was present in strains from species of B. ansorpii, B. bronchialis, B. bronchiseptica, B. flabilis, B. parapertussis, B. pertussis, B. petrii, B. pseudohinzii and some other unclassified strains [13]. RB50 is a reference strain of genus B. bronchiseptica and is investigated extensively [19,21,22,23,24]. Our previous study predicted that B. bronchiseptica RB50 contained a putative pic gene cluster which similar to that of Alcaligenes faecalis JQ135 (13). Although the pic genes from strain RB50 exhibited relatively high similarity to those of strain JQ135, there was no biochemical evidence to confirm the hypothesis.
In this study, the picCRB50 gene was synthesized and its product PicCRB50 was over-expressed, purified, and characterized. The purified PicCRB50 specifically converts 3,6DHPA to 2,5DHP. The optimum conditions and the kinetic properties of PicCRB50 were also characterized. This study inferred that PicCRB50 (BB0271) has 3,6DHPA decarboxylase activity which extended our understanding of PA catabolism by the pathogen Bordetella spp.

2. Materials and Methods

2.1. Chemicals and Culture Media

PA, 2,5DHP, gentisic acid, and 2,3-dihydroxybenzoic acid were purchased from J&K Scientific, Ltd. (Shanghai, China). 3,6DHPA was chemically synthesized using the method as previously [12]. Methanol and formic acid for high performance liquid chromatography (HPLC) were purchased from Merck KgaA (Darmstadt, Germany). Luria-Bertani (LB) consisted of the following components (in g L−1): 10.0 tryptone, 5.0 yeast extract, and 10.0 NaCl. Mineral salt culture medium consists of 1.0 g (NH4)2SO4, 0.5 g KH2PO4·2H2O, 0.2 g MgSO4·7H2O, 1.5 g K2HPO4·3H2O, 1.0 g NaCl, at pH 7.0. The solid media were prepared by adding 20.0 g of agar powder to the above 1.0 L of liquid medium. Phosphate buffer (PBS, pH 7.4) consisted of 8.0 NaCl, 0.2 KCl, 1.42 Na2HPO4, 0.27 KH2PO4 (in g L−1). All other reagents used in this study were commercially available. All media were sterilized by autoclaving at 121 °C for 25 min before use.

2.2. Plasmids and Bacterial Strains

The plasmid pMD-19T (TaKaRa Biotech Co., Ltd., Dalian, China) was used for DNA cloning by using T-A clone method. The plasmid pET29a(+) (Novagen, New York, NY, USA) was used for the expression of the His-tagged 3,6DHPA decarboxylase PicCRB50 form B. bronchiseptica RB50. The plasmids in this study were extracted by RapidLyse Plasmid Mini Kit (Vanzyme, Nanjing, China). Escherichia coli DH5α was used as a host in DNA cloning experiments. E. coli BL21(DE3) strain was used as the host for over-expression of the gene cloned In the pET29a(+). E. coli DH5α and BL21(DE3) competent cells were purchased from TaKaRa Biotech (Dalian, China). E. coli strains were grown in LB medium under aerobic conditions at 37 °C with a rotary shaker 180 rpm.

2.3. Cloning the picCRB50 Gene

The DNA fragment containing picCRB50 (963 bp, gene ID: BB0271 or AYT36_RS01365) was synthesized according to the genome of B. bronchiseptica RB50 [19]. The synthetic product picCRB50 was ligated into plasmid pMD-19T and then sequenced (Shanghai Sangon Biotech Co., Ltd., Shanghai, China) using E. coli DH5α as hosts. The fragment picCRB50 gene without stop coden (960 bp) was amplified using the synthesized DNA as template with primer pairs Prm-picCRB50-F 5′-ACT GCA TAT GAT GAC AAA AGT GCG CAA GAT CGC-3′ (NdeI restriction site underlined) and Prm-picCRB50-R 5′-ACT GCT CGA GCA GTT TGA ACA GGC GCG CGG CGT-3′ (XhoI restriction site underlined). The oligonucleotide primers used in this study were synthesized by a commercial company (Shanghai Sangon Biotech Co., Ltd., Shanghai, China). PCR was performed in a programmable thermocycler. The PCR procedures were an initial denaturing step of 5 min at 95 °C, 30 cycles of 95 °C for 15 s, annealing step at 56 °C for 20 s, elongation step at 72 °C for 1 min and a final elongation step at 72 °C for 5 min. The PCR mixture contained 0.2 U/μL of EX Taq polymerase (TaKaRa, Dalian, China), 0.25 mM concentrations of each deoxynucleoside triphosphate, 0.5 μM concentrations of each primer, 1× buffer, ∼50 ng of DNA, and water to 50 μL. The PCR products were determined by standard gel electrophoresis and purified by the PCR product purification kit (Vanzyme, Nanjing, China). The amplified fragment picCRB50 gene was digested with QuickCut restriction enzymes with a 30 μL reactioin mixture of 10 mM Tris-HCl (pH7.5), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.01% BSA, 0.15% TritonX-100, 50% Glycerol, 1 μg of DNA, 1 μL QuickCut NdeI and 1 μL QuickCut XhoI. The plasmid pET29a(+) were digested with NdeI and XhoI with the same procedures. Then, the above DNA fragements and plasmids were ligated together with the One Step Cloning kit (Clontech, Beijing, China) according to the manufacturer’s instructions. The rsesulting plasmid pET-picCRB50 were then transferred into E. coli BL21(DE3) strain for overexpression of PicCRB50 which contained C-terminally 6× His-tag. The final recombinant plasmid was verified by DNA sequence.

2.4. Expression and Purification PicCRB50

The recombinant plasmid pET-picCRB50 was transformed into E. coli BL21(DE3) for protein overexpression. The BL21(DE3) cell was grown at 37 °C to an optical density OD600 of 0.4 in LB supplemented with 50 mg/L kanamycin. C-terminally His-tagged PicCRB50 expression was induced by the addition of 0.1 mM isopropyl β-D-1stiogalactopyranoside (IPTG) at 16 °C for 12 h when the cell optical density at 600 nm reached to 0.35. The cells were harvested by centrifugation at 12,000 rpm for 5 min, draw away the supernatant, resuspended and washed twice with 50 mM PBS (pH 7.4) buffer. The induced BL21(DE3) cells were re-suspended in the combination buffer (40 mM Tris-Cl, 0.5 M NaCl, and 5 mM imidazole, pH 8.0) and disrupted by sonication (Ultrasonic Cell Crusher XO-900D) in an ice-water bath for 15 min (on for 2 s and off for 3 s). The cell-free extract was removed by centrifugation at 12,000 rpm for 30 min at 4 °C. The supernatants were loaded onto an Ni-NTA (Shanghai Sangon Biotech Co., Ltd., Shanghai, China) column, which was pre-equilibrated with combination buffer with 20 mM imidazole (pH 8.0). The column was eluted with 100 mL, 50 mL, and 10 mL combination buffer of 50 mM, 100 mM, and 300 mM imidazole (pH 8.0), respectively. Purified recombinant PicCRB50 should be dialyzed against buffer to remove imidazole with a Spectra/Por CE dialysis membrane with a molecular weight cutoff of 3500 (Spectrum Laboratories, Inc., Shanghai, China) at 4 °C for 12 h. The purified PicCRB50 was monitored by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In addition, two site-directed mutagenesis of PicCRB50 were constructed and over-expressed, in which histidine residues were changed into alanines, i.e., PicC(H163A) and PicC(H216A). For example, to construct PicC(H163A), the mutant picCRB50 fragments were amplified using plasmid pET-PicCRB50 as DNA templates through overlap PCR. Two primer pairs Prm-picCRB50-F and Mut-H163A-1 (5′-GAC GTG CCG CTG TAC CTG GCG CCG TTC GAC GCC TAC GTG-3′) (alanine encoding codon underlined) and primer pairs Mut-H163A-2 (5′-CAC GTA GGC GTC GAA CGG CGC CAG GTA CAG CGG CAC GTC-3′) (alanine encoding codon underlined) and Prm-picCRB50-R were used to amplify two fragments of picCRB50 gene with the coden of His 163 (CAT) to Ala (GCG). The PCR procedures were set as an initial denaturing step of 5 min at 95 °C, 30 cycles of 95 °C for 15 s, annealing step at 56 °C for 20 s, elongation step at 72 °C for 20 s and a final elongation step at 72 °C for 2 min. After that, two PCR products were purified by the PCR product purification kit (Vanzyme, Nanjing, China) and then used as DNA templates for the second PCR. In the second PCR procedure, there only 10 amplification cycles 95 °C for 15 s, annealing step at 56 °C for 20 s, elongation step at 72 °C for 1 min. Additionally, then Prm-picCRB50-F and Prm-picCRB50-R were added for the third round PCR with procedures of an initial denaturing step of 5 min at 95 °C, 30 cycles of 95 °C for 15 s, annealing step at 56 °C for 20 s, elongation step at 72 °C for 20 s and a final elongation step at 72 °C for 5 min. The final PCR products were purified by the PCR product purification kit and then were ligated into NdeI and XhoI-digested plasmid pET29a(+) via the One Step Cloning kit (Clontech, Beijing, China) according to the manufacturer’s instructions. The reuslting plasmid pET-PicC(H163A) were transferred into E. coli BL21(DE3) strain for overexpression of protein PicC(H163A). Resultant constructs were confirmed by DNA sequencing. The expression and purification of the mutant PicC proteins were performed as described in the section above.

2.5. Enzyme Assay of Purified His-Tagged PicCRB50

For the 3,6DHPA decarboxylase activity, the enzyme reaction mixture contained 50 mM PBS (pH 7.4), 0.2 mM 3,6DHPA, and 10 μg of purified PicCRB50 (in 1 mL) and was incubated at 25 °C. Total protein concentrations were determined using the Bradford method [25]. Conversion of the 3,6DHPA by PicCRB50 was continuously determined by UV/Vis spectrophotometry (Shimadzu, UV-2450, Japan) at 25 °C. The enzymatic activities were calculated spectrophotometrically by the disappearance of 3,6DHPA at 360 nm (ε = 4.4 cm−1 mM−1) according to previous studies [12]. The product 2,5DHP was confirmed via LC-MS/MS analysis as described below. The optimum pH of PicCRB50 was determined using the following buffers: 50 mM citric acid-sodium citrate (pH 4.0 to 6.0), 50 mM KH2PO4-K2HPO4 (pH 6.0 to 8.0), and 50 mM glycine-NaOH (pH 8.0 to 9.8) at 25 °C. The optimum temperature of the PicCRB50 was determined to be between 5 and 60 °C in PBS (pH 7.4). The influences of heavy metals were performed by adding 1 mM Ag+, Ca2+, Co2+, Cu2+, Hg+, Fe3+, Mg2+, Mn2+, Ni2+, Zn2+, and the metal ion chelating agent EDTA, respectively. Purified PicCRB50 was pre-incubated with various metal ions and inhibitors at 4 °C for 30 min to study their effects on the enzyme. The activity was expressed as a percentage of the activity obtained in the absence of the added compounds. To determine the effect of one condition, other conditions were kept at fixed concentration of the standard reaction mixture and the reaction was started by the addition of 3,6DHPA. To determine the kinetic constants for 3,6DHPA, a range of 3,6DHPA concentrations (0 to 300 μM) was used. The values were calculated through nonlinear regression fitting to the Michaelis–Menten equation. One unit of the activity was defined as the amount of enzyme that catalyzed 1 μmol of 3,6DHPA in 1 min at pH 7.0 and 25 °C.

2.6. Analytical Methods

The degradation of the substrates by PicCRB50 was analyzed by High Performance Liquid Chromatography (HPLC) with a C18 reversed phase column (5 μm, 4.60 mm × 250 mm) (Thermo Fisher Scientific, Waltham, MA, USA). The concentrations of the compounds were calculated using standard samples. The mobile phase consisted of methanol, water, and formic acid (12.5:87.5:0.2 [v/v/v]) at a flow rate of 0.8 mL/min at 30 °C. The UV-VIS spectra (260 nm to 400 nm) were observed by an Evolution 201 spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA). LC/TOF-MS analysis was performed in a TripleTOF 5600 (AB SCIEX) mass spectrometer as described previously [26].

3. Results

3.1. Pic Gene Cluster Present in Bordetella spp.

Bordetella spp. strains can cause pertussis or respiratory disease in humans and/or various animals [19,20]. B. bronchiseptica, B. parapertussis, and B. pertussis are three classical Bordetella species which are highly concerned due to their pathogenicity and could invade the respiratory tract of animals or human and cause severe diseases. B. petrii is the only environmental species with remarkable abilities to degrade aromatic compounds isolated from polluted soil, river sediment, or grass root [27]. To date, there are 25, 90, 637, and 1 complete genome sequences released in NCBI genome database in B. bronchiseptica, B. parapertussis, B. pertussis, and B. petrii strains, respectively. After genomic survey, all these Bordetella strains in these four species contains the pic gene cluster (Figure 1 and Figure 2). The protein sequences, taking PicC as an example, of PicC homologues from Bordetella spp. showed 60–70% identities against that of PicC. Phylogenetic tree showed that the PicC of most Bordetella bacteria were clustered into two branches and separated from other species such as Alcaligenes species (Figure 2).

3.2. Organization of Pic Gene Cluster of B. bronchiseptica RB50

B. bronchiseptica strain RB50 is a representative model of Bordetella species which has been studied in depth [19,23,24]. The organizations of the pic gene cluster of strain RB50 were found to be similar to that of A. faecalis JQ135 (Figure 1). For example, picT and picB form a divergent group. picA1A2A3 and picB1B2B3B4 were clustered together similarly. Only a few individual genes are organized differently. For example, in A. faecalis JQ135, the picG gene is the distance from pic cluster, while in B. bronchiseptica RB50, the picG gene is located between picC and picE. The picR gene was separated with two unknown genes from picC gene in B. bronchiseptica RB50. The picGEDF encodes 2,5DHP 5,6-dioxygenase, N-formylmaleamic acid deformylase, maleamic acid amidohydrolase, and maleic acid isomerase, respectively, which catalyze 2,5DHP to fumarate (an intermediate of TCA). The organization of picGEDF of B. bronchiseptica RB50 is consistent with most other 2,5-DHP degradation gene clusters, e.g., hpo cluster from P. putida S16 [28]. The protein sequence similarities of each gene are between 60% and 80% with an exception of PicB2 (47% similarity).

3.3. Cloning and Over-Expression of PicCRB50

The homologous protein of PicC from B. bronchiseptica RB50 (BB0271, WP_003807348.1, designated as PicCRB50) was annotated as amidohydrolase family protein or Amidohydro-rel domain-containing protein in public database (e.g., NCBI). In order to assess the hypothesis, the DNA sequence of picCRB50 gene was synthesized and ligated in plasmid pET29a yielding pET-PicCRB50. The synthesized picCRB50 gene product and the recombinant plasmid pET-PicCRB50 were checked by agarose gel electrophoresis. Then, the pET-PicCRB50 was transferred into E. coli BL21(DE3) and the recombinant 6× His PicCRB50 was over-expressed and purified (Figure 3A). The molecular mass of PicCRB50 was approximately 40.2 kDa and consisted of a single polypeptide as observed by SDS-PAGE.

3.4. The PicCRB50 Catalyzes 3,6DHPA into 2.5DHP

The enzymatic activity of purified PicCRB50 towards 3,6DHPA was first monitored spectrophotometrically with a UV-VIS absorption at 260 nm to 400 nm. As shown in Figure 3B, the UV-VIS absorption curves were changed significantly. The 3,6DHPA consumed with a decrease at maximum 340 nm. As a result, a product accumulated at 250 nm which accord to absorption spectra of 2,5DHP. HPLC results indicated that 3,6DHPA (retention time 9.3 min) was degraded and transformed to a product, which had the same retention time as authentic sample of 2,5DHP (6.4 min) (Figure 3C). LC/TOF-MS analysis showed that the molecular ion peak of the product was 112.0400 (M + H+) which was consistent with standard 2,5DHP (Figure 3D). PicCRB50 showed no activities to gentisic acid and 2,3-dihydroxybenzoic acid which were structural analogues of 3,6DHPA, indicating it specific for 3,6DHPA. In addition, the functions of key histidine residues of PicCRB50 were determined by site-directed mutagenesis. The resultant mutant proteins PicC(H163A) and PicC(H216A) lost the abilities of 3,6DHPA decarboxylase activity completely, indicating these two histidine residues were crucial for PicCRB50.

3.5. Characteristics of the PicCRB50

The optimum pH for PicCRB50 activity was determined at pH 7.0. It retained less than 10% relative activity at pH 3.0 or 10.0 (Figure 4A). The optimum temperature for the PicCRB50 activity was determined to be 35 °C, and PicCRB50 show relatively high activity at temperatures from 20 °C to 40 °C (Figure 4B). PicCRB50 was relatively stable at 35 °C and remains approximately 80% and 40% activity after 2 h and 12 h, respectively. When temperatures were above 40 °C, PicCRB50 lost its stability easily. PicCRB50 was strongly inhibited by 1 mM of the heavy metals Ag+, Cu2+, and Hg+, whereas it was slightly inhibited by Fe3+, Ni2+, Zn2+, and the metal ion chelating agent EDTA. In contrast, Ca2+, Co2+, Mg2+, and Mn2+ significantly increased PicCRB50 activity (Figure 4C). The enzyme kinetics of the recombinant PicCRB50 showed that Km and kcat values for 3,6DHPA were 20.41 + 2.60 μM and 7.61 ± 0.53 S−1, respectively (Figure 4D).

4. Discussion

In this study, the function of 3,6DHPA decarboxylase PicCRB50 of B. bronchiseptica RB50 was investigated in vitro which can transform 3,6DHPA to 2,5DHP. The PicCRB50 exhibited optimal activities at pH 7.0, 35 °C, and the Km and kcat values of PicCRB50 for 3,6DHPA were 20.41 + 2.60 μM and 7.61 ± 0.53 S−1, which were similar with those of PicCJQ135 from A. faecalis JQ135 [12]. The study proved our previous prediction that B. bronchiseptica RB50 contained a pic gene cluster and had the potential to degrade PA [13].
PicCRB50 was annotated as amidohydrolase family protein or Amidohydro-rel domain-containing protein in B. bronchiseptica genome. Previous studies found that picCRB50 gene (BB0271) was adjacent to a conserved cluster whose products catalyze 2,5DHP to fumarate [24]. However, no further investigations or predictions were carried out at that stage. Our present study proved that BB0271 is actually 3,6DHPA decarboxylase. In addition, strain B. bronchiseptica RB50 was reported to be capable of degrading nicotinic acid, in which 6-hydroxy-nicotinic acid and 2,5DHP were key intermediates. In B. bronchiseptica RB50, there were two identical copies of picGEDF genes (≈100% identical in DNA sequences). One was located in pic gene cluster involved in PA degradation based on the present study. The other was located in nic gene cluster involved in nicotinic acid degradation [24]. Rare reports showed two identical gene clusters existing in one bacterium for different pyridine derivatives [13]. The possible reason might be due to a shortcut combination of horizontal gene transfer of picGEDF genes and the same intermediate 2,5DHP.
PA is a natural pyridine-derivative which can be produced both by human/animals and microorganisms [6,7,8]. As reported by previous studies, B. bronchiseptica RB50 and most other Bordetella spp. strains are pathogens of humans, birds, and animal livestock [19,21]. A question raised here is whether Bordetella strains degrade PA when parasitizing the host. In human body, PA is a metabolite of L-tryptophan metabolite and present in various biological issues, such as cell culture supernatants, serum, and human milk [7]. Rare reports have shown PA could be deposed by human. PA was reported as a neuroprotectant and plays a role in the pathogenesis of inflammatory disorders within the central nervous system [29]. In addition, PA is toxic to bacteria. For examples, PA was reported to reduce the degradation efficiency of nitrobenzene and could inhibit sporulation of Bacillus cereus [30]. Thus, if Bordetella strains degrade PA and utilized it as carbon source, the subsequent results could be (i) removing and decomposing the inhibitor on pathogens; (ii) utilizing the inhibitors as nutrients for cell growth; and (iii) enhancing the pathogenicity of Bordetella strains. Therefore, it will be an interesting topic to check whether PA exists in the diseased tissue and can be decomposed by Bordetella strains in vivo.
PA is also produced by microorganisms during degradation of some aromatic compounds. For example, PA was a byproduct of diquator or nitrobenzene [6,8]. Thus, PA comes to nutrients for soil/water bacteria. Bacteria from species B. petrii are isolated from environmental niches, such as polluted soil, river sediment, or grass root [27]. Bioinformatic analyses also showed that B. petrii harbors the pic cluster and the PicC was 84% sequence similarities to PicCRB50. Therefore, we can infer that PA can be degraded and served as nutrients for B. petrii strains. Bacteria in nature often face complex and nutrient-limiting environments [31,32]. Environmental Bordetella strains with pic cluster are bound to survive when facing the PA-containing micro-environments.

Author Contributions

Conceptualization, C.Y. and J.H.; methodology, C.Y., L.Z. and Z.K.; software, L.Z., L.T., L.W. and Y.Y.; validation, L.Z., Z.K., Y.Y. and J.H.; formal analysis, L.Z., L.W. and Y.Y.; investigation, C.Y., L.Z., L.T., L.W., Z.K., Y.Y. and J.H.; resources, C.Y. and J.H.; data curation, L.W. and Z.K.; writing—original draft preparation, C.Y. and J.H.; writing—review and editing, C.Y. and J.H.; visualization, C.Y., L.Z., L.T., L.W., Z.K., Y.Y. and J.H.; supervision, J.H.; project administration, C.Y. and J.H.; funding acquisition, C.Y. and J.H. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the Jiangsu Agricultural Science and Technology Innovation Fund [CX (22) 3128] and National Natural Science Foundation of China (Nos. 32170128 31970096).

Data Availability Statement

The 963-bp picCRB50 gene is avalaible on GenBank under accesion no. of BB0271 or AYT36_RS01365.

Acknowledgments

The authors are grateful to anonymous reviewers for helpful comments on the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Kaiser, J.-P.; Feng, Y.; Bollag, J.-M. Microbial metabolism of pyridine, quinoline, acridine, and their derivatives under aerobic and anaerobic conditions. Microbiol. Rev. 1996, 60, 483–498. [Google Scholar] [CrossRef] [PubMed]
  2. Puripattanavong, J.; Songkram, C.; Lomlim, L.; Amnuaikit, T. Development of concentrated emulsion containing Nicotiana tabacum extract for use as pesticide. J. Appl. Pharmaceut. Sci. 2013, 3, 016–021. [Google Scholar]
  3. Časaitė, V.; Stanislauskienė, R.; Vaitekūnas, J.; Tauraitė, D.; Rutkienė, R.; Gasparavičiūtė, R.; Meškys, R. Microbial degradation of pyridine: A complete pathway in Arthrobacter sp. strain 68b deciphered. Appl. Environ. Microbiol. 2020, 86, e00902–e00920. [Google Scholar] [CrossRef] [PubMed]
  4. Zhang, Y.; Zhang, Y.; Xiong, J.; Zhao, Z.; Chai, T. The enhancement of pyridine degradation by RhodococcusKDPy1 in coking wastewater. FEMS Microbiol. Lett. 2018, 366, fny271. [Google Scholar] [CrossRef]
  5. Guadie, A.; Han, J.-L.; Liu, W.; Ding, Y.-C.; Minale, M.; Ajibade, F.O.; Zhai, S.; Wang, H.-C.; Cheng, H.; Ren, N.; et al. Evaluating the effect of fenton pretreated pyridine wastewater under different biological conditions: Microbial diversity and biotransformation pathways. J. Environ. Manag. 2021, 287, 112297. [Google Scholar] [CrossRef]
  6. Nishino, S.F.; Spain, J.C. Degradation of nitrobenzene by a Pseudomonas pseudoalcaligenes. Appl. Environ. Microbiol. 1993, 59, 2520–2525. [Google Scholar] [CrossRef] [Green Version]
  7. Bryleva, E.Y.; Brundin, L. Kynurenine pathway metabolites and suicidality. Neuropharmacology 2017, 112, 324–330. [Google Scholar] [CrossRef] [Green Version]
  8. Chirino, B.; Strahsburger, E.; Agulló, L.; González, M.; Seeger, M. Genomic and Functional Analyses of the 2-Aminophenol Catabolic Pathway and Partial Conversion of Its Substrate into Picolinic Acid in Burkholderia xenovorans LB400. PLOS ONE 2013, 8, e75746. [Google Scholar] [CrossRef] [Green Version]
  9. Suzuki, K.; Yasuda, M.; Yamasaki, K. Stability Constants of Picolinic and Quinaldic Acid Chelates of Bivalent Metals. J. Phys. Chem. 1957, 61, 229–231. [Google Scholar] [CrossRef]
  10. Donahue, C.J.; Archer, R.D. Transition metal eight-coordination. 8. Stereochemical integrity, geometrical isomers, and isomerization of mixed ligand tungsten (IV) chelates containing picolinic acid and 8-quinolinol derivatives. J. Am. Chem. Soc. 1977, 99, 6613–6623. [Google Scholar] [CrossRef]
  11. Yousef, T.; El-Reash, G.A.; Al-Zahab, M.A.; Safaan, M. Physicochemical investigations, biological studies of the Cr (III), Mn (II), Fe (III), Co (II), Ni (II), Cu (II), Zn (II), Cd (II), Hg (II) and UO2 (VI) complexes of picolinic acid hydrazide derivative: A combined experimental and computational approach. J. Mol. Struct. 2019, 1197, 564–575. [Google Scholar] [CrossRef]
  12. Qiu, J.; Zhang, Y.; Yao, S.; Ren, H.; Qian, M.; Hong, Q.; Lu, Z.; He, J. Novel 3,6-Dihydroxypicolinic Acid Decarboxylase-Mediated Picolinic Acid Catabolism in Alcaligenes faecalis JQ135. J. Bacteriol. 2019, 201, e00665-18. [Google Scholar] [CrossRef]
  13. Qiu, J.; Zhao, L.; Xu, S.; Chen, Q.; Chen, L.; Liu, B.; Hong, Q.; Lu, Z.; He, J. Identification and Characterization of a Novel pic Gene Cluster Responsible for Picolinic Acid Degradation in Alcaligenes faecalis JQ135. J. Bacteriol. 2019, 201, e00077-19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Heyes, M.P.; Chen, C.Y.; Major, E.O.; Saito, K. Different kynurenine pathway enzymes limit quinolinic acid formation by various human cell types. Biochem. J. 1997, 326, 351–356. [Google Scholar] [CrossRef] [PubMed]
  15. Zheng, C.; Wang, Q.; Ning, Y.; Fan, Y.; Feng, S.; He, C.; Zhang, T.C.; Shen, Z. Isolation of a 2-picolinic acid-assimilating bacterium and its proposed degradation pathway. Bioresour. Technol. 2017, 245, 681–688. [Google Scholar] [CrossRef] [Green Version]
  16. Zheng, C.; Zhou, J.; Wang, J.; Qu, B.; Lu, H.; Zhao, H. Aerobic degradation of 2-picolinic acid by a nitrobenzene-assimilating strain: Streptomyces sp. Z2. Bioresour. Technol. 2009, 100, 2082–2084. [Google Scholar] [CrossRef]
  17. Tate, R.L.; Ensign, J.C. Picolinic acid hydroxylase of Arthrobacter picolinophilus. Can. J. Microbiol. 1974, 20, 695–702. [Google Scholar] [CrossRef]
  18. Xu, S.; Wang, X.; Zhang, F.; Jiang, Y.; Zhang, Y.; Cheng, M.; Yan, X.; Hong, Q.; He, J.; Qiu, J. PicR as a MarR Family Transcriptional Repressor Multiply Controls the Transcription of Picolinic Acid Degradation Gene Cluster pic in Alcaligenes faecalis JQ135. Appl. Environ. Microbiol. 2022, 88, e00172-22. [Google Scholar] [CrossRef]
  19. Parkhill, J.; Sebaihia, M.; Preston, A.; Murphy, L.D.; Thomson, N.; Harris, D.E.; Holden, M.T.; Churcher, C.M.; Bentley, S.D.; Mungall, K.L. Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nat. Genet. 2003, 35, 32. [Google Scholar] [CrossRef]
  20. Linz, B.; Ma, L.; Rivera, I.; Harvill, E.T. Genotypic and phenotypic adaptation of pathogens: Lesson from the genus Bordetella. Current. Opinion Infect. Diseas. 2019, 32, 223. [Google Scholar] [CrossRef]
  21. Bridel, S.; Bouchez, V.; Brancotte, B.; Hauck, S.; Armatys, N.; Landier, A.; Mühle, E.; Guillot, S.; Toubiana, J.; Maiden, M.C.J.; et al. A comprehensive resource for Bordetella genomic epidemiology and biodiversity studies. Nat. Commun. 2022, 13, 1–12. [Google Scholar] [CrossRef] [PubMed]
  22. Nash, Z.M.; Cotter, P.A. Regulated, sequential processing by multiple proteases is required for proper maturation and release of Bordetella filamentous hemagglutinin. Mol. Microbiol. 2019, 112, 820–836. [Google Scholar] [CrossRef]
  23. Bone, M.A.; Wilk, A.J.; Perault, A.I.; Marlatt, S.A.; Scheller, E.V.; Anthouard, R.; Chen, Q.; Stibitz, S.; Cotter, P.A.; Julio, S.M. Bordetella PlrSR regulatory system controls BvgAS activity and virulence in the lower respiratory tract. Proc. Natl. Acad. Sci. USA 2017, 114, E1519–E1527. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Brickman, T.J.; Armstrong, S.K. The Bordetella bronchiseptica nic locus encodes a nicotinic acid degradation pathway and the 6-hydroxynicotinate-responsive regulator BpsR. Mol. Microbiol. 2018, 108, 397–409. [Google Scholar] [CrossRef] [Green Version]
  25. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248–254. [Google Scholar] [CrossRef] [PubMed]
  26. Yang, Z.; Jiang, W.; Wang, X.; Cheng, T.; Zhang, D.; Wang, H.; Qiu, J.; Cao, L.; Wang, X.; Hong, Q. An amidase gene ipaH is responsible for the initial degradation step of iprodione in strain Paenarthrobacter sp. YJN-5. Appl. Environ. Microbiol. 2018, 84, 01150-18. [Google Scholar] [CrossRef] [Green Version]
  27. Gross, R.; Guzman, C.A.; Sebaihia, M.; Santos, V.A.P.M.D.; Pieper, D.H.; Koebnik, R.; Lechner, M.; Bartels, D.; Buhrmester, J.; Choudhuri, J.V.; et al. The missing link: Bordetella petrii is endowed with both the metabolic versatility of environmental bacteria and virulence traits of pathogenic Bordetellae. BMC Genom. 2008, 9, 449. [Google Scholar] [CrossRef] [Green Version]
  28. Tang, H.; Yao, Y.; Wang, L.; Yu, H.; Ren, Y.; Wu, G.; Xu, P. Genomic analysis of Pseudomonas putida: Genes in a genome island are crucial for nicotine degradation. Sci. Rep. 2012, 2, 377. [Google Scholar] [CrossRef] [Green Version]
  29. Jhamandas, K.; Boegman, R.; Beninger, R.; Bialik, M. Quinolinate-induced cortical cholinergic damage: Modulation by tryptophan metabolites. Brain Res. 1990, 529, 185–191. [Google Scholar] [CrossRef]
  30. Nakata, H.M.; Halvorson, H.O. Biochemical changes occurring during growth and sporulation of bacillus cereus. J. Bacteriol. 1960, 80, 801–810. [Google Scholar] [CrossRef] [Green Version]
  31. Lowe, S.E.; Jain, M.K.; Zeikus, J.G. Biology, ecology, and biotechnological applications of anaerobic bacteria adapted to environmental stresses in temperature, pH, salinity, or substrates. Microbiol. Rev. 1993, 57, 451–509. [Google Scholar] [CrossRef] [PubMed]
  32. Irving, S.E.; Choudhury, N.R.; Corrigan, R.M. The stringent response and physiological roles of (pp) pGpp in bacteria. Nat. Rev. Microbiol. 2021, 19, 256–271. [Google Scholar] [CrossRef] [PubMed]
Figure 1. Organization of the pic gene clusters of B. bronchiseptica RB50 and A. faecalis JQ135. The bb0268-bb0283 indicate the gene IDs of pic genes in strain RB50. Numbers between the arrows indicate the percent amino acid sequence identities between strains RB50 and JQ135.
Figure 1. Organization of the pic gene clusters of B. bronchiseptica RB50 and A. faecalis JQ135. The bb0268-bb0283 indicate the gene IDs of pic genes in strain RB50. Numbers between the arrows indicate the percent amino acid sequence identities between strains RB50 and JQ135.
Microorganisms 11 00854 g001
Figure 2. Phylogenetic relationships of PicC from strains RB50, JQ135, and other related bacteria. The amino acid sequences of PicCRB50 of B. bronchiseptica strain RB50 was selected for comparisons against the non-redundant protein sequences database in NCBI using Blastp (protein–protein BLAST). The expected (E) value inclusion threshold was 10. Strains containing PicCRB50 homologues with coverage >60% and identity >60% were collected and further assessed for the presence of PicA, PicC, and PicGEDF. Only bacteria containing all pic genes were selected and PicC were used for phylogenetic tree construction. Accession numbers of sequences are given before genus names. Bar, 0.05 substitutions per amino acid residue position. The PicC sequences from Bordetella and Alcaligenes strains were indicated in purple and green, respectively. PicC form strain RB50 (WP_003807348.1) and strain JQ135 (WP_094197645.1) were in red.
Figure 2. Phylogenetic relationships of PicC from strains RB50, JQ135, and other related bacteria. The amino acid sequences of PicCRB50 of B. bronchiseptica strain RB50 was selected for comparisons against the non-redundant protein sequences database in NCBI using Blastp (protein–protein BLAST). The expected (E) value inclusion threshold was 10. Strains containing PicCRB50 homologues with coverage >60% and identity >60% were collected and further assessed for the presence of PicA, PicC, and PicGEDF. Only bacteria containing all pic genes were selected and PicC were used for phylogenetic tree construction. Accession numbers of sequences are given before genus names. Bar, 0.05 substitutions per amino acid residue position. The PicC sequences from Bordetella and Alcaligenes strains were indicated in purple and green, respectively. PicC form strain RB50 (WP_003807348.1) and strain JQ135 (WP_094197645.1) were in red.
Microorganisms 11 00854 g002
Figure 3. Purification and identification of PicCRB50. (A) SDS−PAGE analysis of purified PicCRB50. Lane M, protein marker. A single bond of purified PicCRB50 was indicated. (B) Spectrophotometric monitor of 3,6DHPA consumption (blue) and 2,5DHP formation (red) by purified PicCRB50. (C) HPLC profiles of 3,6DHPA consumption and 2,5DHP formation by purified PicCRB50. (D) LC/TOF−MS profile of the transformation product 2,5DHP.
Figure 3. Purification and identification of PicCRB50. (A) SDS−PAGE analysis of purified PicCRB50. Lane M, protein marker. A single bond of purified PicCRB50 was indicated. (B) Spectrophotometric monitor of 3,6DHPA consumption (blue) and 2,5DHP formation (red) by purified PicCRB50. (C) HPLC profiles of 3,6DHPA consumption and 2,5DHP formation by purified PicCRB50. (D) LC/TOF−MS profile of the transformation product 2,5DHP.
Microorganisms 11 00854 g003
Figure 4. Characterization of PicCRB50. (A) Effect of pH on enzyme activity of PicCRB50. (B) Effect of temperature on enzyme activity of PicCRB50. (C) Effect of metal ions on enzyme activity of PicCRB50. (D) The kinetic curve of PicCRB50. The Data were shown in means ± S.E.M.
Figure 4. Characterization of PicCRB50. (A) Effect of pH on enzyme activity of PicCRB50. (B) Effect of temperature on enzyme activity of PicCRB50. (C) Effect of metal ions on enzyme activity of PicCRB50. (D) The kinetic curve of PicCRB50. The Data were shown in means ± S.E.M.
Microorganisms 11 00854 g004
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Yuan, C.; Zhao, L.; Tong, L.; Wang, L.; Ke, Z.; Yang, Y.; He, J. Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50. Microorganisms 2023, 11, 854. https://doi.org/10.3390/microorganisms11040854

AMA Style

Yuan C, Zhao L, Tong L, Wang L, Ke Z, Yang Y, He J. Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50. Microorganisms. 2023; 11(4):854. https://doi.org/10.3390/microorganisms11040854

Chicago/Turabian Style

Yuan, Cansheng, Lingling Zhao, Lu Tong, Lin Wang, Zhuang Ke, Ying Yang, and Jian He. 2023. "Expression and Characterization of 3,6-Dihydroxy-picolinic Acid Decarboxylase PicC of Bordetella bronchiseptica RB50" Microorganisms 11, no. 4: 854. https://doi.org/10.3390/microorganisms11040854

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop