Pannonibacter anstelovis sp. nov. Isolated from Two Cases of Bloodstream Infections in Paediatric Patients
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. In Vitro Analysis
2.3. 16S rDNA Sanger Sequencing
2.4. Whole-Genome Sequencing
2.5. Bioinformatics Analyses
3. Results
3.1. Isolation, Microscopic, and Biochemical Characterization
3.2. 16S Gene Analysis
3.3. Paired-End Sequencing Analysis
3.4. Oxford Nanopore Technology Re-Sequencing Analysis
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Antimicrobial Molecule | MIC Pt 1 Strain | MIC Pt 2 Strain |
---|---|---|
Amikacin | >32 | >32 |
Cefepime | 16 | 16 |
Cefotaxime | 16 | 16 |
Ceftazidime | 32 | 32 |
Ciprofloxacin | 0.5 | 0.5 |
Gentamycin | >8 | >8 |
Imipenem | 0.5 | 0.5 |
Meropenem | 1 | 1 |
Piperacillin/Tazobactam | 16 | 16 |
Tobramycin | >8 | >8 |
Well | Biochemical Tests | Results |
---|---|---|
0 | Control | − |
1 | Glycerol | − |
2 | Erythritol | − |
3 | D-Arabinose | W |
4 | L-Arabinose | + |
5 | D-Ribose | − |
6 | D-Xylose | + |
7 | L-Xylose | W |
8 | D-Adonitol | − |
9 | Methyl-βD-Xylopyranoside | − |
10 | D-Galactose | W |
11 | D-Glucose | W |
12 | D-Fructose | W |
13 | D-Mannose | − |
14 | L-Sorbose | − |
15 | L-Rhamnose | − |
16 | Dulcitol | − |
17 | Inositol | − |
18 | D-Mannitol | − |
19 | D-Sorbitol | − |
20 | Methyl-αD-Mannopyranoside | − |
21 | Methyl-αD-Glucopyranoside | − |
22 | N-AcetylGlucosamine | − |
23 | Amygdalin | − |
24 | Arbutin | − |
25 | Esculin ferric citrate | + |
26 | Salicin | − |
27 | D-Cellobiose | W |
28 | D-Maltose | − |
29 | D-Lactose | − |
30 | D-Melibiose | − |
31 | D-Saccharose (sucrose) | − |
32 | D-Trehalose | − |
33 | Inulin | − |
34 | D-Melezitose | − |
35 | D-Raffinose | − |
36 | Amidon (starch) | − |
37 | Glycogen | − |
38 | Xylitol | − |
39 | Gentiobiose | − |
40 | D-Turanose | − |
41 | D-Lyxose | − |
42 | D-Tagatose | − |
43 | D-fucose | + |
44 | L-fucose | W |
45 | D-Arabitol | − |
46 | L-Arabitol | − |
47 | Potassium Gluconate | − |
48 | Potassium 2-KetoGluconate | − |
49 | Potassium 5-KetoGluconate | − |
Well | Biochemical Tests | Results |
---|---|---|
1 | ONPG | + |
2 | ADH | − |
3 | LDC | − |
4 | ODC | − |
5 | Citrate | + |
6 | Hydrogen sulphide | − |
7 | Urease | − |
8 | TDA | − |
9 | Indole | − |
10 | Voges-Proskauer | − |
11 | Gelatin | − |
12 | Nitrate | − |
Sample | Pt1 (MiSeq) | Pt2 (MiSeq) | Pt2 (ONT) | Pannonibacter phragmitetus DSM 14782 (GCA_000382365.1) |
---|---|---|---|---|
Pt1 (MiSeq) | - | 99.99 (99.71) | 99.76 (99.61) | 91.21 (85.76) |
Pt2(MiSeq) | 100.00 (99.81) | - | 99.77 (99.65) | 91.23 (85.58) |
Pt2-lr (ONT) | 99.78 (99.61) | 99.78 (99.67) | - | 91.16 (85.67) |
Pannonibacter phragmitetus DSM 14782 (GCA_000382365.1) | 91.16 (83.33) | 91.16 (83.26) | 91.08 (83.29) | - |
Protein Accession | InterPRO Retained Domain | Antimicrobial Putative Interaction |
---|---|---|
FGIJAEJN_03410 | Penicillin-binding protein, dimerisation domain | Cefepime, Cefotaxime, Ceftazidime, Piperacillin |
FGIJAEJN_01305, FGIJAEJN_01921 | Penicillin-binding protein, transpeptidase | Cefepime, Cefotaxime, Ceftazidime, Piperacillin |
FGIJAEJN_02309, FGIJAEJN_03733, FGIJAEJN_01441, FGIJAEJN_01366 | Beta-lactamase/transpeptidase-like | Cefepime, Cefotaxime, Ceftazidime, Piperacillin |
FGIJAEJN_02847 | Penicillin-binding protein transglycosylase domain | Cefepime, Cefotaxime, Ceftazidime, Piperacillin |
FGIJAEJN_00520 | Penicillin-binding protein, OB-like domain | Cefepime, Ceftazidime |
FGIJAEJN_00222 | Penicillin-binding protein, C-terminal domain superfamily | Piperacillin |
FGIJAEJN_03193, FGIJAEJN_01113, FGIJAEJN_03499, FGIJAEJN_02312, FGIJAEJN_02878, FGIJAEJN_00760 | Aminoglycoside phosphotransferase | Aminoglycoside |
FGIJAEJN_00577, FGIJAEJN_02821, FGIJAEJN_02760, FGIJAEJN_03969, FGIJAEJN_00577, FGIJAEJN_03969 | Drug resistance transporter Bcr/CmlA subfamily | Bicyclomycin, Chloramphenicol, Florfenicol |
FGIJAEJN_01136, FGIJAEJN_00166 | Chloramphenicol acetyltransferase-like domain superfamily | Chloramphenicol |
FGIJAEJN_02702, FGIJAEJN_03166, FGIJAEJN_01924 | Glyoxalase/fosfomycin resistance/dioxygenase domain | Fosfomycin |
FGIJAEJN_02566 | Multi antimicrobial extrusion protein | Multidrug |
FGIJAEJN_00274 | Multiple antibiotic resistance (MarC)-related | Multidrug |
FGIJAEJN_00230 | Drug resistance transporter EmrB-like | Multidrug |
FGIJAEJN_00589 | Small drug/metabolite transporter protein family | Multidrug |
FGIJAEJN_03037, FGIJAEJN_00275, FGIJAEJN_01585, FGIJAEJN_04086, FGIJAEJN_03816, FGIJAEJN_00630 | Multidrug efflux transporter AcrB TolC docking domain, DN/DC subdomains | Multidrug |
FGIJAEJN_00589 | Small multidrug resistance protein | Multidrug |
FGIJAEJN_04073 | Peptidase M74, penicillin-insensitive murein endopeptidase | Penicillin |
FGIJAEJN_02114 | Penicillin-binding, C-terminal | Penicillin |
FGIJAEJN_00838 | PBP domain | Penicillin |
FGIJAEJN_02207 | Beta-lactamase-related | Penicillin, Cephamycin, Cephalosporin |
FGIJAEJN_01279 | AmpG-like permease/Acetyl-coenzyme A transporter 1 | Penicillin, Cephamycin, Cephalosporin |
FGIJAEJN_02140 | Beta-lactamase, class-A active site | Penicillin, Cephamycin, Cephalosporin |
FGIJAEJN_00577 | Tetracycline resistance protein TetA/multidrug resistance protein MdtG | Tetracycline |
FGIJAEJN_00237 | Tetracycline repressor TetR, C-terminal | Tetracycline |
FGIJAEJN_04089 | Tetracyclin repressor-like, C-terminal domain | Tetracycline |
FGIJAEJN_01776, FGIJAEJN_03091, FGIJAEJN_04181, FGIJAEJN_02679, FGIJAEJN_00245, FGIJAEJN_02722, FGIJAEJN_02364, FGIJAEJN_00123, FGIJAEJN_02082, FGIJAEJN_02903, FGIJAEJN_00246, FGIJAEJN_02749 | Tetracyclin repressor-like, C-terminal domain superfamily | Tetracycline |
FGIJAEJN_03224 | PsrA, tetracyclin repressor-like, C-terminal domain | Tetracycline |
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Castellana, S.; De Laurentiis, V.; Bianco, A.; Del Sambro, L.; Grassi, M.; De Leonardis, F.; Derobertis, A.M.; De Carlo, C.; Sparapano, E.; Mosca, A.; et al. Pannonibacter anstelovis sp. nov. Isolated from Two Cases of Bloodstream Infections in Paediatric Patients. Microorganisms 2024, 12, 799. https://doi.org/10.3390/microorganisms12040799
Castellana S, De Laurentiis V, Bianco A, Del Sambro L, Grassi M, De Leonardis F, Derobertis AM, De Carlo C, Sparapano E, Mosca A, et al. Pannonibacter anstelovis sp. nov. Isolated from Two Cases of Bloodstream Infections in Paediatric Patients. Microorganisms. 2024; 12(4):799. https://doi.org/10.3390/microorganisms12040799
Chicago/Turabian StyleCastellana, Stefano, Vittoriana De Laurentiis, Angelica Bianco, Laura Del Sambro, Massimo Grassi, Francesco De Leonardis, Anna Maria Derobertis, Carmen De Carlo, Eleonora Sparapano, Adriana Mosca, and et al. 2024. "Pannonibacter anstelovis sp. nov. Isolated from Two Cases of Bloodstream Infections in Paediatric Patients" Microorganisms 12, no. 4: 799. https://doi.org/10.3390/microorganisms12040799