Ultrasound-Attenuated Microorganisms Inoculated in Vegetable Beverages: Effect of Strains, Temperature, Ultrasound and Storage Conditions on the Performances of the Treatment
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganisms
2.2. Ultrasound Treatments
2.3. Viability and Acidification of Attenuated Strains in Vegetable Beverages
2.4. Effect of Thermal Abuse on Lactiplantibacillus plantarum c16 and c19 Attenuated by US and Inoculated in Rice Beverage
3. Results
3.1. Viability and Acidifying Capability of Attenuated Strains in Vegetable Beverages
3.2. Effect of Thermal Abuse on Lactiplantibacillus plantarum c16 and c19 Attenuated by US and Inoculated in Rice Beverage
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
Lb. | Lactobacillus |
L. | Lactiplantibacillus |
Lc. | Lacticaseibacillus |
Li. | Limosilactobacillus |
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Combinations | Power (%) | Time (Minutes) | Pulse (Seconds) |
---|---|---|---|
G | 40 | 2 | 2 |
H | 40 | 4 | 2 |
I | 40 | 6 | 2 |
O | 60 | 2 | 2 |
N (Control) | - | - | - |
Code | Treatments |
---|---|
A | US treatment |
B | Control |
C | US treatment; storage at 30 °C for 4 h |
D | Control; storage at 30 °C for 4 h |
E | US treatment; storage at 30 °C for 24 h |
F | Control; storage at 30 °C for 24 h |
SS | Degree of Freedom | MS | F | p | |
---|---|---|---|---|---|
Intercept | 725.69 | 1 | 725.69 | 36,596.77 | 0.00 |
{1} strains | 9.027 | 3 | 3.01 | 151.75 | 0.00 |
{2} combinations | 13.53 | 4 | 3.38 | 170.54 | 0.00 |
{3} beverages | 88.85 | 3 | 29.62 | 1493.53 | 0.00 |
{4} time | 287.64 | 3 | 95.88 | 4835.34 | 0.00 |
Strains x combinations | 9.12 | 12 | 0.76 | 38.31 | 0.00 |
Strains x beverages | 68.52 | 9 | 7.61 | 383.94 | 0.00 |
Combinations x beverages | 12.69 | 12 | 1.06 | 53.32 | 0.00 |
Strains x time | 3.86 | 9 | 0.43 | 21.65 | 0.00 |
Combinations x time | 6.66 | 12 | 0.56 | 28.01 | 0.00 |
Beverages x time | 35.55 | 9 | 3.95 | 199.19 | 0.00 |
Strains x combinations x beverages | 19.99 | 36 | 0.55 | 28.01 | 0.00 |
Strains x combinations x time | 4.87 | 36 | 0.13 | 6.82 | 0.00 |
Strains x beverages x time | 35.26 | 27 | 1.31 | 65.86 | 0.00 |
Combinations x beverages x time | 10.22 | 36 | 0.28 | 14.32 | 0.00 |
1 × 2 × 3 × 4 | 21.66 | 108 | 0.20 | 10.11 | 0.00 |
Error | 6.34 | 320 | 0.02 |
SS | Degr. of Freedom | MS | F | p | |
---|---|---|---|---|---|
Intercept | 3368.37 | 1 | 3368.37 | 184,038.30 | 0.00 |
{1}temperature | 79.04 | 2 | 39.52 | 2159.20 | 0.00 |
{2}time | 702.23 | 5 | 140.45 | 7673.60 | 0.00 |
{3}treatment | 47.63 | 5 | 9.53 | 520.50 | 0.00 |
{4}strains | 1.21 | 1 | 1.21 | 66.20 | 0.00 |
Temperature x time | 16.79 | 10 | 1.68 | 91.70 | 0.00 |
Temperature x treatment | 33.49 | 10 | 3.35 | 183.00 | 0.00 |
Time x treatment | 14.28 | 25 | 0.57 | 31.20 | 0.00 |
Temperature x strains | 0.36 | 2 | 0.18 | 9.90 | 0.00 |
Time x strains | 17.41 | 5 | 3.48 | 190.30 | 0.00 |
Treatment x strains | 11.97 | 5 | 2.39 | 130.80 | 0.00 |
Temperature x time x treatment | 9.97 | 50 | 0.20 | 10.90 | 0.00 |
Temperature x time x strains | 1.14 | 10 | 0.11 | 6.20 | 0.00 |
Temperature x treatment x strains | 1.58 | 10 | 0.16 | 8.60 | 0.00 |
Time x treatment x strains | 4.96 | 25 | 0.20 | 10.80 | 0.00 |
1 × 2 × 3 × 4 | 3.20 | 50 | 0.06 | 3.50 | 0.00 |
Error | 3.95 | 216 | 0.02 |
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Share and Cite
Campaniello, D.; Corbo, M.R.; Speranza, B.; Sinigaglia, M.; Bevilacqua, A. Ultrasound-Attenuated Microorganisms Inoculated in Vegetable Beverages: Effect of Strains, Temperature, Ultrasound and Storage Conditions on the Performances of the Treatment. Microorganisms 2020, 8, 1219. https://doi.org/10.3390/microorganisms8081219
Campaniello D, Corbo MR, Speranza B, Sinigaglia M, Bevilacqua A. Ultrasound-Attenuated Microorganisms Inoculated in Vegetable Beverages: Effect of Strains, Temperature, Ultrasound and Storage Conditions on the Performances of the Treatment. Microorganisms. 2020; 8(8):1219. https://doi.org/10.3390/microorganisms8081219
Chicago/Turabian StyleCampaniello, Daniela, Maria Rosaria Corbo, Barbara Speranza, Milena Sinigaglia, and Antonio Bevilacqua. 2020. "Ultrasound-Attenuated Microorganisms Inoculated in Vegetable Beverages: Effect of Strains, Temperature, Ultrasound and Storage Conditions on the Performances of the Treatment" Microorganisms 8, no. 8: 1219. https://doi.org/10.3390/microorganisms8081219