Novel Y RNA-Derived Fragments Can Differentiate Canine Hepatocellular Carcinoma from Hepatocellular Adenoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Population (Clinical Samples)
2.2. Cell Lines and Cell Culture
2.3. EV Isolation
2.4. RNA Isolation of Clinical Samples and HCC Cell Lines
2.5. ncRNAs Selection and qRT-PCR
2.6. Statistical Analysis
3. Results
3.1. Expression of Y RNA Using qRT-PCR
3.1.1. Relative Expression in the Clinical Tissue Samples
3.1.2. Relative Expression in Plasma
3.1.3. Relative Expression in Plasma EVs
3.1.4. Relative Expression in Canine HCC Cell Lines
3.2. Diagnostic Value of Y RNA
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Number | Age | Disease | Sex | Neutered | Breed | Tissue | Plasma |
---|---|---|---|---|---|---|---|
P1 | 11 Y 3 M | HCA | M | Yes | Crossbreed | 15 | 5 |
P2 | 8 Y 1 M | HCA | F | Yes | Miniature dachshund | ||
P3 | 10 Y 7 M | HCA | M | Yes | Toy poodle | ||
P4 | 12 Y 2 M | HCA | F | Yes | Shiba | ||
P5 | 11 Y 6 M | HCA | M | No | Miniature dachshund | ||
P6 | 12 Y 3 M | HCA | M | No | Miniature dachshund | ||
P7 | 11 Y 9 M | HCA | M | No | Crossbreed | ||
P8 | 13 5 M | HCA | F | Yes | Miniature dachshund | ||
P9 | 14 Y | HCA | M | No | Golden retriever | ||
P10 | 9 Y 2 M | HCA | F | Yes | Toy poodle | ||
P11 | 13 Y | HCA | F | Yes | Jack Russell terrier | ||
P12 | 11 Y 1 M | HCA | M | No | Miniature dachshund | ||
P13 | 12 Y 2 M | HCA | M | No | Crossbreed | ||
P14 | 12 Y 3 M | HCA | M | No | Crossbreed | ||
P15 | 10 Y 7 M | HCA | M | No | Shiba | ||
P16 | 12 Y 3 M | HCC | F | No | Chihuahua | 13 | 9 |
P17 | 11 Y 3 M | HCC | F | Yes | Miniature dachshund | ||
P18 | 14 Y | HCC | F | Yes | Crossbreed | ||
P19 | 10 Y 8 M | HCC | M | Yes | Shiba | ||
P20 | 11 Y 7 M | HCC | M | Yes | Welsh corgi | ||
P21 | 10 Y 9 M | HCC | F | No | Crossbreed | ||
P22 | 10 Y 3 M | HCC | F | No | Beagle | ||
P23 | 10 Y 9 M | HCC | F | No | Yorkshire terrier | ||
P24 | 11 Y 6 M | HCC | M | No | Shiba | ||
P25 | 12 Y | HCC | F | No | Miniature schnauzer | ||
P26 | 11 Y 10 M | HCC | M | No | Yorkshire terrier | ||
P27 | 13 Y 10 M | HCC | F | No | Shetland sheepdog | ||
P28 | 11 Y 7 M | HCC | M | Yes | Crossbreed |
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Ushio, N.; Hasan, M.N.; Arif, M.; Miura, N. Novel Y RNA-Derived Fragments Can Differentiate Canine Hepatocellular Carcinoma from Hepatocellular Adenoma. Animals 2023, 13, 3054. https://doi.org/10.3390/ani13193054
Ushio N, Hasan MN, Arif M, Miura N. Novel Y RNA-Derived Fragments Can Differentiate Canine Hepatocellular Carcinoma from Hepatocellular Adenoma. Animals. 2023; 13(19):3054. https://doi.org/10.3390/ani13193054
Chicago/Turabian StyleUshio, Norio, Md Nazmul Hasan, Mohammad Arif, and Naoki Miura. 2023. "Novel Y RNA-Derived Fragments Can Differentiate Canine Hepatocellular Carcinoma from Hepatocellular Adenoma" Animals 13, no. 19: 3054. https://doi.org/10.3390/ani13193054