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Article

Risk Factors Including Age, Stage and Anatomic Location that Impact the Outcomes of Patients with Synovial Sarcoma

by
Minggui Pan
1,2,* and
Maqdooda Merchant
2
1
Department of Oncology and Hematology, Kaiser Permanente, 710 Lawrence Expressway, Santa Clara, CA 95051, USA
2
Kaiser Permanente Division of Research, Oakland, CA 94612, USA
*
Author to whom correspondence should be addressed.
Med. Sci. 2018, 6(1), 21; https://doi.org/10.3390/medsci6010021
Submission received: 3 January 2018 / Revised: 10 February 2018 / Accepted: 1 March 2018 / Published: 6 March 2018
(This article belongs to the Section Cancer and Cancer-Related Research)
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Abstract

Synovial sarcoma is a high-grade soft tissue sarcoma that inflicts mostly children and young adults with high mortality rate; however, the risk factors that impact the outcomes remain incompletely understood. We have identified the synovial sarcoma cases from the Kaiser Permanente Northern California cancer registry between 1981 and 2014. Kaplan–Meier plots were used to display disease-free survival (DFS) and overall survival (OS); log-rank tests and Cox proportional hazard models were used to determine the impact of clinical factors on DFS, OS, and disease-specific survival. Tumor size > 5.0 cm and age > 50 years were associated with higher risk of presenting with stage IV disease. Median OS for patients with stage IV was 1.3 years and 7.8 years for early-stage disease. For patients with early-stage disease, tumor size > 5.0 cm was significantly associated with worse DFS, sarcoma-specific morality, and OS. Compared to extremity primary, patients with head and neck and trunk primary had approximately three-fold higher sarcoma-specific mortality and lower OS. There was no significant difference in DFS or OS among three histologic subtypes. Pre-operative and/or post-operative chemotherapy was not associated with improvement in DFS or OS. Twenty-six patients relapsed with predominantly lung metastasis, thirteen of whom received metastatectomy with a median OS of 7.8 years, compared to 2.3 years for patients who did not receive metastatectomy. In conclusion, age older than 50 years and tumor size > 5.0 cm are risk factors for presenting with stage IV disease. For early-stage patients, trunk and head and neck primary as well as tumor size > 5.0 cm are risk factors for decreased OS.

1. Introduction

Synovial sarcoma is a high-grade soft tissue sarcoma (STS) with three histological subtypes (biphasic, monophasic, and poorly differentiated) that are characterized by variable degree of spindle and epithelial cell differentiation [1], and by a specific chromosomal translocation t(X:18)(p11:q11) [2,3,4]. The chromosomal translocation involves three variants of SSX (SSX1, SSX2, and SSX4) fused to the SYT gene (SS18) [5,6,7,8]. Mechanistically, the fusion protein SS18-SSX displaces wild-type SS18 protein from the BAF (Brg1-associated factor) complex, reversing the polycomb-mediated repression of SOX2 gene transcription, leading to deregulated expression of SOX2—a transcription factor that regulates a large repertoire of genes critical in modulating cell growth and differentiation [9]. The three different fusion variants are similarly distributed in all three histologic subtypes, and were shown to be of some prognostic significance [10,11]. Both SYT-SSX1 and SYT-SSX2 have been shown to be equally potent in driving synovial sarcomagenesis in mouse models [12].
Synovial sarcoma occurs most frequently in the lower extremities, while it is less common in the upper extremities, head and neck and trunk [1,13]. For resectable early-stage disease, the main-stay of treatment approach is surgical resection, followed by adjuvant radiation with or without adjuvant chemotherapy [14]. In patients with locally-advanced tumor invading critical surrounding structures such as vessel or nerve, pre-operative radiation and/or chemotherapy may be used to downstage the disease first, followed by surgical resection. Some clinical features such as tumor size, margin status, histologic grade, age, sex, and bone and vascular invasion have been shown to be associated with outcomes, with larger tumor size (>5.0 cm) being consistently shown to be associated with shorter disease-free survival (DFS) and overall survival (OS) [11,15,16,17,18,19]. Some studies suggested that primary site may be associated with outcomes as well, but results were not consistent [15]. The benefit of adjuvant chemotherapy has been controversial in STS as a whole [14,20,21]. No synovial sarcoma-specific randomized clinical trial for adjuvant chemotherapy has been performed. Additionally, controversies remain regarding the benefit of metastatectomy in metastatic synovial sarcoma.
This is an observational study of patients with synovial sarcoma to investigate the clinical risk factors associated with stage IV disease at presentation and DFS, sarcoma-specific mortality, and OS in patients with early-stage synovial sarcoma.

2. Materials and Methods

Synovial sarcoma cases were identified from the Kaiser Permanente Northern California (KPNC) cancer registry between 1981 and 2014. The KPNC is a large integrated healthcare delivery system providing comprehensive medical care to more than 4 million members. The Kaiser Foundation Research Institute’s Institutional Review Board approved this study with waiver of consent (No. CN-14-2018-H), and funding was by a grant from the KPNC Community Benefit Program.
We identified a total of 154 cases that were histologically-confirmed synovial sarcoma between 1981 and 2014 through the electronic database of KPNC, and 130 of these cases were stage I to III. One case was excluded from the analysis due to a lack of relevant information in the medical chart. The diagnosis of all cases was confirmed by either typical chromosomal translocation and/or by expert sarcoma pathologists in academic institutions where the cases were submitted for consultation (including Stanford University, Mayo Clinic, Emory University, and Harvard Medical School). The majority of cases (approximately 90%) were confirmed by the typical gene rearrangement. The cytogenetic study was performed in the Regional Genetic Laboratory of KPNC that revealed the characteristic chromosomal translocation but did not identify the specific gene fusion variant [4]. The immunohistochemical staining was performed by the Regional Immunohistochemistry Laboratory of KPNC. Magnetic resonance imaging (MRI) was routinely performed for the initial assessment of the primary tumor and subsequent surveillance in most cases. Positron emission tomography/computed tomography (PET/CT) scan or CT scan of chest, abdomen, and pelvis was routinely performed for initial workup and surveillance every 3 to 6 months in the majority of cases.
Definition of primary site: proximal lower extremity includes gluteal, groin, thigh areas; distal lower extremity includes knee and below; proximal upper extremity includes shoulder, axilla, and arm; distal upper extremity includes elbow and below. Head and neck primary site includes maxillary sinus, infratemporal fossa, thyroid, floor of mouth, larynx, and neck; trunk includes axil, chest and abdominal wall, lung, pleura, mediastinum, pericardium, retroperitoneum, and infranephric area.
Statistical analysis: We compared demographic and clinical characteristics of the cases by early-stage and stage IV as well as by receipt of pre- and/or post-operative chemotherapy. All categorical variables were analyzed using the chi-square test and numerical variables were analyzed using the non-parametric Wilcoxon test and results presented with medians and interquartile ranges. In addition, Kaplan–Meier curves were created to display DFS and OS, and log-rank tests were used to compare differences between various strata such as early-stage versus stage IV disease and primary sites. Cox proportional hazard models were used to determine the impact of clinical characteristics such as tumor size, chemotherapy, and primary site on DFS, OS, and disease-specific survival after adjusting for age, gender, and race. All analyses were conducted using SAS version 9.3 (SAS Institute Inc., Cary, NC, USA).

3. Results

3.1. Immunohistochemical Staining Characteristics

To understand the pattern of immunohistochemical staining, we examined 43 cases of early-stage (35 cases) and stage IV (8 cases) synovial sarcoma diagnosed from 2006 to 2014. These cases had a more complete panel of immunostaining performed compared to the older cases. We presented the results of the most commonly performed markers in these cases (Table 1). BCL2 staining was positive in all 26 cases. Approximately 94% of cases stained positive for CD99 with a cytoplasmic and membranous staining pattern that was weaker and more diffuse than the typically homogeneous membranous staining seen in Ewing’s sarcoma. Pan-cytokeratin stained positive in approximately 80% of cases, while EMA 62%. Thirty-three percent stained positive for S100. Only one out of 33 cases (3%) stained positive for CD34. SMA and desmin stained negative in 100% of the cases stained. All 24 cases (100%) stained for Ki67 showed positive staining in a subset of tumor cells (ranging 5–50%).

3.2. Risk Factors Associated with Stage IV Disease at Presentation

The median length of follow-up for all cases was 7 years (range: 1 to 32 years). The age at the diagnosis ranged from 7 to 86 years old. Twenty-three cases (15%) presented with stage IV disease (metastatic disease) and with a median age of 50 (interquartile range (IQR) = 32)—significantly older than the median age of 36 (IQR = 25) for the patients with early-stage disease (stage I to III) (p = 0.02, Table 2). Sex and race/ethnicity distribution were not associated with stage of disease. For patients with stage IV disease, 70% presented with tumor size > 5.0 cm, compared to 53% for patients who presented with early-stage disease (p < 0.0001, Table 2). The median OS for patients with stage IV (2 years) was much shorter than that (25 years) of early-stage (Figure 1).

3.3. Risk Factors Associated with Poor Outcomes in Early-Stage Synovial Sarcoma

Next, we investigated the clinical features of the 130 early-stage patients and their association with DFS, sarcoma-specific mortality (sarcoma mortality), and all-cause mortality (mortality) (Table 3). Only four out of 130 cases were superficial disease. Eighteen cases (13.8%) underwent amputation, while 112 cases (86.2%) received limb-preservation surgery. Among the 112 cases who received limb-preservation surgery, eighty-one cases (72%) received either pre-operative or post-operative radiation, while thirty-one cases (28%) did not. The cases who did not receive radiation had a median tumor size of 3.0 cm, compared to 6.3 cm for the cases who received adjuvant radiation, indicating that smaller tumor size was the primary factor for not receiving radiation for the majority of patients.
Using Cox regression model and mutually controlling for demographic variables age, race, gender and clinical variables tumor size, whether chemotherapy was given or not, primary disease location, and histology in multivariate analysis, we found that tumor size > 5.0 was significantly associated with worse DFS (hazardous ratio (HR) = 2.9, p = 0.002), sarcoma-specific mortality (HR = 3.4, p = 0.003), and all-cause mortality (HR = 2.8, p = 0.003), compared to tumor size < 5.0 cm. In addition, compared to the patients with extremity and girdle synovial sarcoma, we found no difference in DFS for the patients with head and neck primary site as well as trunk primary site (Table 3). However, patients with head and neck and trunk primary site had significantly higher sarcoma-specific mortality (HR = 2.8, p = 0.04; HR = 3.2, p = 0.01) and all-cause mortality (HR = 2.5, p = 0.04; HR = 2.4, p = 0.03) (Table 3). No outcome difference was found between biphasic and monophasic histologic subtypes (Table 3). The median DFS and OS were 4.4, 3.6, and 2.6 years and 8.6, 7.9, and 3.3 years for extremity, head and neck, and trunk primary sites, respectively (Figure 2 and Figure 3).

3.4. Impact of Chemotherapy on Outcomes of Patients with Early-Stage Synovial Sarcoma

Among 130 patients with early-stage disease, forty received pre- and/or post-operative chemotherapy; among them, 30 (75%) had extremity or girdle primary. Eight (25%) of the 40 patients who received chemotherapy had amputation, while thirty-two (75%) had limb-preservation surgery, of which all received either pre-operative or adjuvant radiation. There was no statistically significant difference in age, sex, ethnicity, tumor size, primary site, or histologic type distribution between the patients who did and did not receive chemotherapy, though the patients who received chemotherapy had a higher percentage of T2 disease compared to the patients who did not receive chemotherapy (p = 0.07, Table 4). Twenty-two patients (55%) received pre-operative chemotherapy, 15 patients (37.5%) received post-operative chemotherapy, and 3 (7.5%) received both. The majority of patients (95%) received ifosfomide and adriamycin-based chemotherapy with a median number of four cycles (Table 5). Kaplan–Meier estimates showed no significant difference in DFS and OS between the patients who did and the patients who did not receive chemotherapy (Figure 4 and Figure 5). There was no difference in sarcoma-specific mortality (28% with no chemotherapy versus 35% with chemotherapy, p = 0.4) and all-cause mortality (35% with no chemotherapy versus 40% with chemotherapy, p = 0.5). Twenty-nine patients (72.5%) who received chemotherapy and 43 patients (52.2%) who did not receive chemotherapy had tumor size > 5.0 cm, and when the analysis was restricted to these patients, no significant difference in DFS and OS was found (data not shown).

3.5. Metastatectomy and Overall Survival of Patients with Lung Metastasis

Twenty-six patients had relapsed disease predominantly to the lungs after the initial treatment for early-stage synovial sarcoma; of these, 13 (50%) had metastatectomy. Most patients had multiple metastatectomy procedures performed. One patient had metastatectomy 8 times, three patients each had each 5, 4, and 3 such surgeries, and another three patients each had 2. One patient had 2 metastatectomies plus two radiofrequency ablation (RFA) procedures performed. Five patients had one metastatectomy only. Thirteen cases had disease in the lungs that was not resectable and did not receive metastatectomy. The OS for the patients treated with metastatectomy was 7.8 years, compared to 2.3 years for the patients not treated with metastatectomy, suggesting that metastatectomy may improve OS.

4. Discussion

Synovial sarcoma continues to be a very challenging disease to manage. The clinical workup often starts with biopsy of a suspicious mass that is typically round or oval with heterogeneous enhancement on imaging studies [22,23] followed by immunohistochemical staining of the biopsy specimen with a variable set of markers. All (100%) of the cases in our cohort stained were positive for BCL2, which is consistent with the previous studies [24,25]. BCL2 overexpression was the result of transcriptional activation rather than gene fusion or increased gene copy number [24]. More than 90% of our cases stained positive for CD99; however, unlike the strong and homogeneous membranous staining typically seen in Ewing’s sarcoma (or primitive neuroectodermal tumor), the CD99 staining in synovial sarcoma is usually weaker and more diffuse with both cytoplasmic and membranous distribution. CD99 is thought to inhibit neural differentiation, and may contribute to oncogenesis in Ewing’s sarcoma [26]. It is not clear if CD99 contributes to the oncogenesis of synovial sarcoma. Our data provide some helpful insight for the initial immunohistochemical evaluation of biopsy specimens that includes synovial sarcoma as a differential diagnosis.
The five-year survival rate of synovial sarcoma ranges from 37% to 90% in the literature [18,27,28,29]. Several clinical factors have been studied to characterize their impact on the prognosis. In a European non-randomized study of pediatric patients with synovial sarcoma, patients were grouped into low-, intermediate-, and high-risk based on tumor size and resectability. Twenty-six patients in the low-risk category with tumor size < 5.0 cm who were treated with surgery alone had 5-year event-free survival (EFS) at approximately 80% and OS at 90%, while 67 patients with high-risk features (larger unresectable tumor, axil primary, or with nodal involvement) had much worse EFS and OS [30]. Tumor size and primary tumor location have been shown to be significantly associated with DFS in several other studies [11,15,31,32,33]. Some suggested that distal or proximal location of extremity may be associated with difference in OS [15,17,33]. Our study did not find any outcome difference between distal and proximal extremity location (data not shown). While the correlative clinical characteristics in many studies are inconsistent, larger tumor size has been consistently shown to be the most significant prognostic factor secondary to metastasis at presentation. Consistent with this, our study with 130 cases of early-stage synovial sarcoma showed that tumor size > 5.0 cm was associated with approximately three-fold worse DFS, sarcoma-specific mortality, and all-cause mortality.
The 23 cases in our cohort who presented with stage IV had a poor OS of 1.3 years, similar to the previous studies [34,35]. The median age of patients presenting with stage IV disease was 50 years old—significantly older than the median age of the 130 cases who presented with early-stage disease (36 years old). The tumor size > 5.0 cm was significantly associated with the risk of presenting with stage IV disease. The trunk location (including axil primary site) appears to be associated with higher proportion of stage IV disease at presentation, but it was not statistically significant. We found that although there were no significant differences in DFS among extremity, head and neck, and trunk primary location, the head and neck primary and trunk primary were associated with approximately 2.5-fold higher sarcoma-specific mortality and all-cause mortality, compared to extremity and girdle primary site. This result appears consistent with the studies by Ferrari et al. [30,36].
The benefit of pre- and post-operative chemotherapy for STS has been an intense debate over the last two decades. There is no randomized clinical trial of pre- or post-operative chemotherapy specifically for synovial sarcoma. Only one randomized prospective trial, conducted by the Italian Sarcoma Group (ISG), showed OS advantage with adjuvant chemotherapy using epirubicin and ifosfomide in patients with extremity and girdle high-grade STS [37]. Other trials (mainly two large EORTC trials) have largely failed to demonstrate the benefit of adjuvant chemotherapy [38,39], though the meta-analyses have shown modest OS benefit [21,40,41]. The ISG followed-up with another randomized trial in pre-operative setting that tested three versus five cycles of chemotherapy, and showed that three cycles were not inferior [42]. A retrospective study of more than 300 cases of synovial sarcoma by Canter et al. with a pre-operative nomogram predicted an early survival benefit with ifosfomide and adriamycin-based chemotherapy [11]. Some other retrospective studies focusing specifically on synovial sarcoma also suggested survival benefit from adjuvant chemotherapy [29,43,44], though one study showed no benefit [33]. In our study, 40 patients with early-stage synovial sarcoma received pre- and/or post-operative chemotherapy, and showed no improvement in either DFS or OS. The higher percentage of T2 disease in the patients who received chemotherapy may or may not have influenced the outcomes. Since all 32 cases who had limb-preservation surgery and chemotherapy also received radiation, radiation would not be a factor influencing the benefit of chemotherapy. However, given its retrospective nature, the data can only be suggestive, not conclusive. A randomized chemotherapy trial specifically for synovial sarcoma in neoadjuvant or adjuvant setting would be of great significance.
The question about the benefit of metastatectomy remains inconclusive [35]. In our cohort, 13 of 26 patients who relapsed with predominantly lung metastasis received metastatectomy and had an OS of 7.8 years, compared to 2.3 years of patients who did not receive metastatectomy. In a previous study of 29 patients who received metastatectomy, five-year OS was 58.4% [45]. In a study of pediatric patients with synovial sarcoma (<22 years old), there was suggestion of improved OS with metastatectomy [33]. Improved OS with lung metastatectomy has been shown in other STS histologies [46,47]. However, the improved OS for patients who underwent metastatectomies could be due to their disease burden being more limited, rather than the procedure itself.
The limitations of our study include that it is a retrospective study with the data spanning more than 30 years, and the number of cases treated with chemotherapy was relatively small. The strengths of our study include the relatively large cohort of cases from a large integrated health care system, the long follow-up duration for most of the cases, and that the majority of patients who received chemotherapy were given a doxorubicin and ifosfomide-based regimen.

5. Conclusions

Our study showed that older age and large tumor size (>5.0 cm) are risk factors for presenting with stage IV disease and that tumor size > 5.0 cm, head and neck as well trunk primary are risk factors for poor survival in patients with early-stage disease. Future studies should continue to explore for the benefit of pre- and post-operative chemotherapy with newer agents.

Acknowledgments

We thank Mary Anne Armstrong and Laurel Habel for valuable suggestions. This work was supported by a grant from the Kaiser Permanente Northern California Community Benefit Program to M.P. and by Jiayan Foundation.

Author Contributions

M.P. conceived the concept; M.P. and M.M. planned the study design, performed data extraction and analysis, and prepared the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Fisher, C. Synovial sarcoma. Ann. Diagn. Pathol. 1998, 2, 401–421. [Google Scholar] [CrossRef] [Scilit]
  2. Clark, J.; Rocques, P.J.; Crew, A.J.; Gill, S.; Shipley, J.; Chan, A.M.; Gusterson, B.A.; Cooper, C.S. Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma. Nat. Genet. 1994, 7, 502–508. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  3. Kawai, A.; Woodruff, J.; Healey, J.H.; Brennan, M.F.; Antonescu, C.R.; Ladanyi, M. SYT-SSX gene fusion as a determinant of morphology and prognosis in synovial sarcoma. N. Engl. J. Med. 1998, 338, 153–160. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  4. Amary, M.F.; Berisha, F.; Bernardi Fdel, C.; Herbert, A.; James, M.; Reis-Filho, J.S.; Fisher, C.; Nicholson, A.G.; Tirabosco, R.; Diss, T.C.; et al. Detection of SS18-SSX fusion transcripts in formalin-fixed paraffin-embedded neoplasms: Analysis of conventional RT-PCR, qRT-PCR and dual color FISH as diagnostic tools for synovial sarcoma. Mod. Pathol. 2007, 20, 482–496. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  5. Cagle, L.A.; Mirra, J.M.; Storm, F.K.; Roe, D.J.; Eilber, F.R. Histologic features relating to prognosis in synovial sarcoma. Cancer 1987, 59, 1810–1814. [Google Scholar] [CrossRef] [Scilit]
  6. Crew, A.J.; Clark, J.; Fisher, C.; Gill, S.; Grimer, R.; Chand, A.; Shipley, J.; Gusterson, B.A.; Cooper, C.S. Fusion of SYT to two genes, SSX1 and SSX2, encoding proteins with homology to the Kruppel-associated box in human synovial sarcoma. EMBO J. 1995, 14, 2333–2340. [Google Scholar] [PubMed]
  7. Skytting, B.; Nilsson, G.; Brodin, B.; Xie, Y.; Lundeberg, J.; Uhlen, M.; Larsson, O. A novel fusion gene, SYT-SSX4, in synovial sarcoma. J. Natl. Cancer Inst. 1999, 91, 974–975. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  8. Reeves, B.R.; Smith, S.; Fisher, C.; Warren, W.; Knight, J.; Martin, C.; Chan, A.M.; Gusterson, B.A.; Westbury, G.; Cooper, C.S. Characterization of the translocation between chromosomes X and 18 in human synovial sarcomas. Oncogene 1989, 4, 373–378. [Google Scholar] [PubMed]
  9. Kadoch, C.; Crabtree, G.R. Reversible disruption of mSWI/SNF (BAF) complexes by the SS18-SSX oncogenic fusion in synovial sarcoma. Cell 2013, 153, 71–85. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  10. Stegmaier, S.; Leuschner, I.; Poremba, C.; Ladenstein, R.; Kazanowska, B.; Ljungman, G.; Scheer, M.; Blank, B.; Bielack, S.; Klingebiel, T.; et al. The prognostic impact of SYT-SSX fusion type and histological grade in pediatric patients with synovial sarcoma treated according to the CWS (Cooperative Weichteilsarkom Studie) trials. Pediatr. Blood Cancer 2016, 64, 89–95. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  11. Canter, R.J.; Qin, L.X.; Maki, R.G.; Brennan, M.F.; Ladanyi, M.; Singer, S. A synovial sarcoma-specific preoperative nomogram supports a survival benefit to ifosfamide-based chemotherapy and improves risk stratification for patients. Clin. Cancer Res. 2008, 14, 8191–8197. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  12. Jones, K.B.; Barrott, J.J.; Xie, M.; Haldar, M.; Jin, H.; Zhu, J.F.; Monument, M.J.; Mosbruger, T.L.; Langer, E.M.; Randall, R.L.; et al. The impact of chromosomal translocation locus and fusion oncogene coding sequence in synovial sarcomagenesis. Oncogene 2016, 35, 5021–5032. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  13. Okcu, M.F.; Munsell, M.; Treuner, J.; Mattke, A.; Pappo, A.; Cain, A.; Ferrari, A.; Casanova, M.; Ozkan, A.; Raney, B. Synovial sarcoma of childhood and adolescence: A multicenter, multivariate analysis of outcome. J. Clin. Oncol. 2003, 21, 1602–1611. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  14. Von Mehren, M.; Randall, R.L.; Benjamin, R.S.; Boles, S.; Bui, M.M.; Conrad, E.U., 3rd; Ganjoo, K.N.; George, S.; Gonzalez, R.J.; Heslin, M.J.; et al. Soft Tissue Sarcoma, Version 2.2016, NCCN Clinical Practice Guidelines in Oncology. J. Natl. Compr. Cancer Netw. JNCCN 2016, 14, 758–786. [Google Scholar] [CrossRef] [Scilit]
  15. Campbell, C.; Gallagher, J.; Dickinson, I. Synovial sarcoma—Towards a simplified approach to prognosis. ANZ J. Surg. 2004, 74, 727–731. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  16. Guillou, L.; Coindre, J.; Gallagher, G.; Terrier, P.; Gebhard, S.; de Saint Aubain Somerhausen, N.; Michels, J.; Jundt, G.; Vince, D.R.; Collin, F.; et al. Detection of the synovial sarcoma translocation t(X;18) (SYT;SSX) in paraffin-embedded tissues using reverse transcriptase-polymerase chain reaction: A reliable and powerful diagnostic tool for pathologists. A molecular analysis of 221 mesenchymal tumors fixed in different fixatives. Hum. Pathol. 2001, 32, 105–112. [Google Scholar] [PubMed]
  17. Deshmukh, R.; Mankin, H.J.; Singer, S. Synovial sarcoma: The importance of size and location for survival. Clin. Orthop. Relat. Res. 2004, 155–161. [Google Scholar] [CrossRef] [Scilit]
  18. Spillane, A.J.; A’Hern, R.; Judson, I.R.; Fisher, C.; Thomas, J.M. Synovial sarcoma: A clinicopathologic, staging, and prognostic assessment. J. Clin. Oncol. 2000, 18, 3794–3803. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  19. Krieg, A.H.; Hefti, F.; Speth, B.M.; Jundt, G.; Guillou, L.; Exner, U.G.; von Hochstetter, A.R.; Cserhati, M.D.; Fuchs, B.; Mouhsine, E.; et al. Synovial sarcomas usually metastasize after > 5 years: A multicenter retrospective analysis with minimum follow-up of 10 years for survivors. Ann. Oncol. 2011, 22, 458–467. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  20. Benjamin, R.S. Evidence for using adjuvant chemotherapy as standard treatment of soft tissue sarcoma. Semin. Radiat. Oncol. 1999, 9, 349–351. [Google Scholar] [CrossRef] [Scilit]
  21. Italiano, A.; Penel, N.; Robin, Y.M.; Bui, B.; Le Cesne, A.; Piperno-Neumann, S.; Tubiana-Hulin, M.; Bompas, E.; Chevreau, C.; Isambert, N.; et al. Neo/adjuvant chemotherapy does not improve outcome in resected primary synovial sarcoma: A study of the French Sarcoma Group. Ann. Oncol. 2009, 20, 425–430. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  22. Baheti, A.D.; Tirumani, S.H.; Sewatkar, R.; Shinagare, A.B.; Hornick, J.L.; Ramaiya, N.H.; Jagannathan, J.P. Imaging features of primary and metastatic extremity synovial sarcoma: A single institute experience of 78 patients. Br. J. Radiol. 2015, 88. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  23. Bakri, A.; Shinagare, A.B.; Krajewski, K.M.; Howard, S.A.; Jagannathan, J.P.; Hornick, J.L.; Ramaiya, N.H. Synovial sarcoma: Imaging features of common and uncommon primary sites, metastatic patterns, and treatment response. AJR Am. J. Roentgenol. 2012, 199, W208–W215. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  24. Mancuso, T.; Mezzelani, A.; Riva, C.; Fabbri, A.; Dal Bo, L.; Sampietro, G.; Perego, P.; Casali, P.; Zunino, F.; Sozzi, G.; et al. Analysis of SYT-SSX fusion transcripts and bcl-2 expression and phosphorylation status in synovial sarcoma. Lab. Investig. J. Tech. Methods Pathol. 2000, 80, 805–813. [Google Scholar] [CrossRef] [Scilit]
  25. Hirakawa, N.; Naka, T.; Yamamoto, I.; Fukuda, T.; Tsuneyoshi, M. Overexpression of BCL-2 protein in synovial sarcoma: A comparative study of other soft tissue spindle cell sarcomas and an additional analysis by fluorescence in situ hybridization. Hum. Pathol. 1996, 27, 1060–1065. [Google Scholar] [CrossRef] [Scilit]
  26. Rocchi, A.; Manara, M.C.; Sciandra, M.; Zambelli, D.; Nardi, F.; Nicoletti, G.; Garofalo, C.; Meschini, S.; Astolfi, A.; Colombo, M.P.; et al. CD99 inhibits neural differentiation of human Ewing sarcoma cells and thereby contributes to oncogenesis. J. Clin. Investig. 2010, 120, 668–680. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  27. Choong, P.; Pritchard, D.; Sim, F.; Rock, M.; Nascimento, A. Long-term survival in high-grade soft-tissue sarcoma—Prognostic factors in synovial sarcoma. Int. J. Oncol. 1995, 7, 161–169. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  28. Hawkins, W.G.; Gold, J.S.; Dyall, R.; Wolchok, J.D.; Hoos, A.; Bowne, W.B.; Srinivasan, R.; Houghton, A.N.; Lewis, J.J. Immunization with DNA coding for gp100 results in CD4 T-cell independent antitumor immunity. Surgery 2000, 128, 273–280. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  29. Brecht, I.B.; Ferrari, A.; Int-Veen, C.; Schuck, A.; Mattke, A.C.; Casanova, M.; Bisogno, G.; Carli, M.; Koscielniak, E.; Treuner, J. Grossly-resected synovial sarcoma treated by the German and Italian Pediatric Soft Tissue Sarcoma Cooperative Groups: Discussion on the role of adjuvant therapies. Pediatr. Blood Cancer 2006, 46, 11–17. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  30. Ferrari, A.; De Salvo, G.L.; Brennan, B.; van Noesel, M.M.; de Paoli, A.; Casanova, M.; Francotte, N.; Kelsey, A.; Alaggio, R.; Oberlin, O.; et al. Synovial sarcoma in children and adolescents: The European Pediatric Soft Tissue Sarcoma Study Group prospective trial (EpSSG NRSTS 2005). Ann. Oncol. 2015, 26, 567–572. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  31. Ferrari, A.; de Salvo, G.L.; Oberlin, O.; Casanova, M.; de Paoli, A.; Rey, A.; Minard, V.; Orbach, D.; Carli, M.; Brennan, B.; et al. Synovial sarcoma in children and adolescents: A critical reappraisal of staging investigations in relation to the rate of metastatic involvement at diagnosis. Eur. J. Cancer 2012, 48, 1370–1375. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  32. Ferrari, A.; Gronchi, A.; Casanova, M.; Meazza, C.; Gandola, L.; Collini, P.; Lozza, L.; Bertulli, R.; Olmi, P.; Casali, P.G. Synovial sarcoma: A retrospective analysis of 271 patients of all ages treated at a single institution. Cancer 2004, 101, 627–634. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  33. Stanelle, E.J.; Christison-Lagay, E.R.; Healey, J.H.; Singer, S.; Meyers, P.A.; La Quaglia, M.P. Pediatric and adolescent synovial sarcoma: Multivariate analysis of prognostic factors and survival outcomes. Ann. Surg. Oncol. 2013, 20, 73–79. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  34. Bergh, P.; Meis-Kindblom, J.M.; Gherlinzoni, F.; Berlin, O.; Bacchini, P.; Bertoni, F.; Gunterberg, B.; Kindblom, L.G. Synovial sarcoma: Identification of low and high risk groups. Cancer 1999, 85, 2596–2607. [Google Scholar] [CrossRef] [Scilit]
  35. Spurrell, E.L.; Fisher, C.; Thomas, J.M.; Judson, I.R. Prognostic factors in advanced synovial sarcoma: An analysis of 104 patients treated at the Royal Marsden Hospital. Ann. Oncol. 2005, 16, 437–444. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  36. Ferrari, A.; Bisogno, G.; Alaggio, R.; Cecchetto, G.; Collini, P.; Rosolen, A.; Meazza, C.; Indolfi, P.; Garaventa, A.; de Sio, L.; et al. Synovial sarcoma of children and adolescents: The prognostic role of axial sites. Eur. J. Cancer 2008, 44, 1202–1209. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  37. Frustaci, S.; Gherlinzoni, F.; de Paoli, A.; Bonetti, M.; Azzarelli, A.; Comandone, A.; Olmi, P.; Buonadonna, A.; Pignatti, G.; Barbieri, E.; et al. Adjuvant chemotherapy for adult soft tissue sarcomas of the extremities and girdles: Results of the Italian randomized cooperative trial. J. Clin. Oncol. 2001, 19, 1238–1247. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  38. Pinedo, H.M.; Bramwell, V.H.; Mouridsen, H.T.; Somers, R.; Vendrik, C.P.; Santoro, A.; Buesa, J.; Wagener, T.; van Oosterom, A.T.; van Unnik, J.A.; et al. Cyvadic in advanced soft tissue sarcoma: A randomized study comparing two schedules. A study of the EORTC Soft Tissue and Bone Sarcoma Group. Cancer 1984, 53, 1825–1832. [Google Scholar] [CrossRef] [Scilit]
  39. Woll, P.J.; Reichardt, P.; Le Cesne, A.; Bonvalot, S.; Azzarelli, A.; Hoekstra, H.J.; Leahy, M.; van Coevorden, F.; Verweij, J.; Hogendoorn, P.C.; et al. Adjuvant chemotherapy with doxorubicin, ifosfamide, and lenograstim for resected soft-tissue sarcoma (EORTC 62931): A multicentre randomised controlled trial. Lancet Oncol. 2012, 13, 1045–1054. [Google Scholar] [CrossRef] [Scilit]
  40. Pervaiz, N.; Colterjohn, N.; Farrokhyar, F.; Tozer, R.; Figueredo, A.; Ghert, M. A systematic meta-analysis of randomized controlled trials of adjuvant chemotherapy for localized resectable soft-tissue sarcoma. Cancer 2008, 113, 573–581. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  41. Collaboration, S.M.-A. Adjuvant chemotherapy for localised resectable soft-tissue sarcoma of adults: Meta-analysis of individual data. Sarcoma Meta-analysis Collaboration. Lancet 1997, 350, 1647–1654. [Google Scholar]
  42. Gronchi, A.; Stacchiotti, S.; Verderio, P.; Ferrari, S.; Martin Broto, J.; Lopez-Pousa, A.; Llombart-Bosch, A.; Dei Tos, A.P.; Collini, P.; Jurado, J.C.; et al. Short, full-dose adjuvant chemotherapy (CT) in high-risk adult soft tissue sarcomas (STS): Long-term follow-up of a randomized clinical trial from the Italian Sarcoma Group and the Spanish Sarcoma Group. Ann. Oncol. 2016, 27, 2283–2288. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  43. Chen, Y.; Yang, Y.; Wang, C.; Shi, Y. Adjuvant chemotherapy decreases and postpones distant metastasis in extremity stage IIB/III synovial sarcoma patients. J. Surg. Oncol. 2012, 106, 162–168. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  44. Eilber, F.C.; Brennan, M.F.; Eilber, F.R.; Eckardt, J.J.; Grobmyer, S.R.; Riedel, E.; Forscher, C.; Maki, R.G.; Singer, S. Chemotherapy is associated with improved survival in adult patients with primary extremity synovial sarcoma. Ann. Surg. 2007, 246, 105–113. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  45. Lee, K.; Kang, M.C.; Lee, H.W.; Park, J.H.; Baek, H.J.; Cho, S.J.; Jeon, D.G. Pulmonary metastasectomy in adult patients with synovial sarcoma: A single-center experience. Korean J. Thorac. Cardiovasc. Surg. 2016, 49, 451–455. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  46. Burt, B.M.; Ocejo, S.; Mery, C.M.; Dasilva, M.; Bueno, R.; Sugarbaker, D.J.; Jaklitsch, M.T. Repeated and aggressive pulmonary resections for leiomyosarcoma metastases extends survival. Ann. Thorac. Surg. 2011, 92, 1202–1207. [Google Scholar] [CrossRef] [Scilit] [PubMed]
  47. Smith, R.; Pak, Y.; Kraybill, W.; Kane, J.M., 3rd. Factors associated with actual long-term survival following soft tissue sarcoma pulmonary metastasectomy. Eur. J. Surg. Oncol. 2009, 35, 356–361. [Google Scholar] [CrossRef] [Scilit] [PubMed]
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Figure 1. Kaplan–Meier estimates of overall survival of patients with stage IV and early-stage synovial sarcoma.
Figure 1. Kaplan–Meier estimates of overall survival of patients with stage IV and early-stage synovial sarcoma.
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Figure 2. Kaplan–Meier estimates of overall survival of early-stage patients with extremity, head and neck, and trunk primary.
Figure 2. Kaplan–Meier estimates of overall survival of early-stage patients with extremity, head and neck, and trunk primary.
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Figure 3. Kaplan–Meier estimates of disease-free survival of early-stage patients with extremity, head and neck, and trunk primary site.
Figure 3. Kaplan–Meier estimates of disease-free survival of early-stage patients with extremity, head and neck, and trunk primary site.
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Figure 4. Kaplan–Meier estimates of disease-free survival of early-stage patients who did and did not receive chemotherapy.
Figure 4. Kaplan–Meier estimates of disease-free survival of early-stage patients who did and did not receive chemotherapy.
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Figure 5. Kaplan–Meier estimates of overall survival of early-stage patients who did and did not receive chemotherapy.
Figure 5. Kaplan–Meier estimates of overall survival of early-stage patients who did and did not receive chemotherapy.
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Table 1. Immunostaining characteristics from 43 cases of early-stage and stage IV synovial sarcoma between 2006 and 2014.
Table 1. Immunostaining characteristics from 43 cases of early-stage and stage IV synovial sarcoma between 2006 and 2014.
PositiveNegative% Positive
BCL2 (N = 26)260100
CD99 (N = 32)30294
Pan-cytokeratin (N = 35)28780
EMA (N = 21)13862
S100 (N = 39)132633
CD34 (N = 33)1323
SMA (N = 38)0380
Desmin (N = 37)0370
Ki67 (N = 24)240100
Table 2. Characteristics of patients with stage IV and early-stage synovial sarcoma.
Table 2. Characteristics of patients with stage IV and early-stage synovial sarcoma.
Stage IV (N = 23 (%))Early-Stage (N = 130 (%))p-Value
Median age (IQR)50 (32)36.5 (25)0.02 *
Sex
 Female11 (48)49 (38)0.36 **
Race
 Asian4 (17)17 (13)0.87 **
 Black1 (4)11 (8)
 Hispanic5 (22)25 (19)
 White13 (57)77 (60)
Tumor size (centimeter)
 <5.01 (4)57 (44)<0.0001 **
 >5.016 (70)69 (53)
 Unknown6 (26)4 (3)
Primary Site
 EXTREMITY13 (57)93 (72)0.10 **
 HEAD_NECK1 (4)12 (9)
 TRUNK9 (39)25 (19)
Histologic type
 Biphasic5 (22)50 (38)0.18 **
 Monophasic13 (56)67 (52)
 Poorly Differentiated3 (13)7 (5)
 Unknown2 (9)6 (5)
IQR: interquartile range; * p-value determined using non-parametric Wilcoxon test; ** p-value determined using Χ2 or Fisher’s exact test.
Table 3. Impact of tumor size, primary site, and histology on disease-free survival (DFS), sarcoma-specific mortality, and all-cause mortality for patients with early-stage synovial sarcoma.
Table 3. Impact of tumor size, primary site, and histology on disease-free survival (DFS), sarcoma-specific mortality, and all-cause mortality for patients with early-stage synovial sarcoma.
CharacteristicDFSSarcoma MortalityAll-Cause Mortality
HR * (CI)p-ValueHR * (CI)p-ValueHR * (CI)p-Value
Tumor size >5.0 cm vs. <5.0 cm2.9 (1.5–5.5)0.0023.4 (1.5–7.5)0.0032.8 (1.4–5.8)0.003
Primary Site
Head and neck vs. extremity1.2 (0.5–2.9)0.7592.8 (1.0–7.5)0.0492.5 (1.0–5.9)0.04
Trunk vs. extremity1.6 (0.8–3.3)0.2203.2 (1.3–7.7)0.0122.4 (1.1–5.5)0.03
Histology: biphasic vs. monophasic0.6 (0.3–1.2)0.1390.8 (0.4–1.5)0.4380.7 (0.4–1.3)0.2
* Hazardous ratio (HR) was adjusted for age, gender, race, and whether chemotherapy was given. CI: confidence interval.
Table 4. Characteristics of early-stage patients who did and did not receive chemotherapy.
Table 4. Characteristics of early-stage patients who did and did not receive chemotherapy.
Chemotherapy Given (N = 40)Chemotherapy not Given (N = 90)p-Value
Median Age (IQR)36 (29.5)38.5 (25)0.39
Sex (%)
 Female12 (30.0)37 (41.1)0.23
Race (%)
 Asian6 (15.0)11 (12.2)0.59
 Black3 (7.5)8 (8.9)
 Hispanic5 (12.5)20 (22.2)
 White26 (65.0)51 (56.7)
Tumor size (centimeter)
 <5.012 (30.0)45 (50.0)0.07
 >=5.027 (67.5)42 (46.7)
 Unknown1 (2.5)3 (3.3)
Primary Site
 EXTREMITY30 (75.0)63 (70.0)0.83
 HEAD_NECK3 (7.5)9 (10.0)
 TRUNK7 (17.5)18 (20.0)
Histology
 Biphasic16 (40.0)34 (37.8)0.80
 Monophasic20 (50.0)47 (52.5)
 Poorly Differentiated3 (7.5)4 (4.4)
 Unknown1 (2.5)5 (5.6)
Table 5. Chemotherapy types and number of cycles received by patients with early-stage synovial sarcoma (A: adriamycin; I: ifosfomide; M: MESNA; V: vincristine; D: dactinomycin).
Table 5. Chemotherapy types and number of cycles received by patients with early-stage synovial sarcoma (A: adriamycin; I: ifosfomide; M: MESNA; V: vincristine; D: dactinomycin).
Type of ChemotherapyFrequency (N = 40 (%))
AIM30 (75)
Adriamycin/cyclophosphomide5 (12.5)
Adriamycin only1 (2.5)
MAID1(2.5)
MAIV1 (2.5)
VID1 (2.5)
Dactinomycin/vincristine1 (2.5)
Number of CyclesFrequency (N = 40 (%))
11 (2.5)
24 (10)
314 (35.0)
412 (30.0)
53 (7.5)
66 (15)
AIM: adriamycin plus ifosfomide and MESNA; MAID: MESNA plus adriamycin and ifosfomide and dactinomycin; MAIV: MESNA plus adriamycin and ifosfomide and vincristine; VID: Vincristine plus ifosfomide and dactinomycin.

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Pan, M.; Merchant, M. Risk Factors Including Age, Stage and Anatomic Location that Impact the Outcomes of Patients with Synovial Sarcoma. Med. Sci. 2018, 6, 21. https://doi.org/10.3390/medsci6010021

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Pan M, Merchant M. Risk Factors Including Age, Stage and Anatomic Location that Impact the Outcomes of Patients with Synovial Sarcoma. Medical Sciences. 2018; 6(1):21. https://doi.org/10.3390/medsci6010021

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Pan, Minggui, and Maqdooda Merchant. 2018. "Risk Factors Including Age, Stage and Anatomic Location that Impact the Outcomes of Patients with Synovial Sarcoma" Medical Sciences 6, no. 1: 21. https://doi.org/10.3390/medsci6010021

APA Style

Pan, M., & Merchant, M. (2018). Risk Factors Including Age, Stage and Anatomic Location that Impact the Outcomes of Patients with Synovial Sarcoma. Medical Sciences, 6(1), 21. https://doi.org/10.3390/medsci6010021

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