Laser Scribed Graphene Biosensor for Detection of Biogenic Amines in Food Samples Using Locally Sourced Materials
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Electrode Fabrication
2.3. Copper Electrodeposition
2.4. Material Characterization
2.5. Sensor Biofunctionalization
2.6. Electrochemical Characterization
2.7. Analysis of Fermented Fish
2.8. Statistical Analysis
3. Results and Discussion
3.1. LSG Morphology
3.2. Material Analysis
3.3. Electrochemical Characterization
3.4. Sensor Biofunctionalization and Calibration
3.5. Analysis of Fish Samples
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Material | Feature Size (Morphology) | Material Identified in Features (keV) | 1 Peak Redox Current (µA) | 2 ESA (cm2) | 3 HET Constant (-) |
---|---|---|---|---|---|
LSG | NA | C (0.28 keV) O (0.54 keV) | ≈100 | 0.34 ± 0.05 | 0.0030 ± 0.0011 |
4 LSG + nCU (CuSo4 source) | 25 to 300 nm (urchin, crystallite) | Cu (0.92 keV) Cu (8.1 keV) | ≈500 | 0.46 ± 0.06 | 0.0038 ± 0.0017 |
4 LSG + nCU (local source) | 500 to 1000 nm (rod, mesotube) | Cu (0.92 keV) Cu (8.1 keV) K (3.3 keV) Cl (2.6 keV) Fe (6.97 keV) | ≈280 | 0.50 ± 0.21 | 0.0033 ± 0.0020 |
Sensor Conformation | Food Sample (BA) | Sensitivity (µA/mM) | LOD (µM) | t95 (s) | Reference |
---|---|---|---|---|---|
DAO/Pt-NP/Graphene/Chi/SPE | fish (His) | 63.1 | 0.02 | NR | Apetrei [48] |
DAO-HRP/polysulfone/CNT/ ferrocene/SPE | fish (His) | 19 | 0.17 | 20 | Pérez [51] |
DAO/photoHEMA/SPE | prawn (His) | 0.62 | 5.8 | 50 | Keow [52] |
DAO/CeO2-PANI/GCE | prawn (His) | 51.47 | 48.7 | 1 | Gumpu [50] |
DAO/Nafion/MnO2/SPE | chicken (His/Tyr) | 5.95 | 3.0 | NR | Telsnig [53] |
DAO-HRP/SPE | fish (His) | 17.66 | 0.18 | NR | Alonso-Lomillo [54] |
HMD/TTF/SPCE | octopus (His/Put) | 10.2 | 8.1 | NR | Henao-Escobar [55] |
LSG-nCu-CNC/DAO (analytical grade materials) | fish (His) | 58.7 ± 5.9 | 7.7 ± 2.8 | 4.1 ± 1.7 | This work |
LSG-Cu-MFC/DAO (locally sourced materials) | fish (His) | 23.3 ± 1.9 | 11.6 ± 2.6 | 7.3 ± 2.5 | This work |
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Vanegas, D.C.; Patiño, L.; Mendez, C.; Oliveira, D.A.d.; Torres, A.M.; Gomes, C.L.; McLamore, E.S. Laser Scribed Graphene Biosensor for Detection of Biogenic Amines in Food Samples Using Locally Sourced Materials. Biosensors 2018, 8, 42. https://doi.org/10.3390/bios8020042
Vanegas DC, Patiño L, Mendez C, Oliveira DAd, Torres AM, Gomes CL, McLamore ES. Laser Scribed Graphene Biosensor for Detection of Biogenic Amines in Food Samples Using Locally Sourced Materials. Biosensors. 2018; 8(2):42. https://doi.org/10.3390/bios8020042
Chicago/Turabian StyleVanegas, Diana C., Laksmi Patiño, Connie Mendez, Daniela Alves de Oliveira, Alba M. Torres, Carmen L. Gomes, and Eric S. McLamore. 2018. "Laser Scribed Graphene Biosensor for Detection of Biogenic Amines in Food Samples Using Locally Sourced Materials" Biosensors 8, no. 2: 42. https://doi.org/10.3390/bios8020042