Estetrol Inhibits Endometriosis Development in an In Vivo Murine Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Handling
2.2. Endometriosis Surgical Induction
2.3. Experimental Design and Sampling of Biological Materials
2.4. Macroscopic Study of Endometriotic-like Lesions
2.5. Proliferating Cell Nuclear Antigen (PCNA) Immunohistochemistry
2.6. Terminal Deoxynucleotidyl Transferase-Mediated Nick End Labeling (TUNEL) Assay
2.7. Antioxidant Enzyme Activities
2.8. Measurement of TBARS-MDA
2.9. Enzyme-Linked Immunosorbent Assay (ELISA)
2.10. RNA Extraction and Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR)
2.11. Statistical Analysis
3. Results
3.1. Effect of E4 on the Growth of Endometriotic-like Lesions
3.2. Effect of E4 on Cell Proliferating in Endometriotic-like Lesions
3.3. Effect of E4 on Cell Death in Endometriotic-like Lesions
3.4. Effect of E4 on Oxidative Stress in Endometriotic-like Lesions
3.5. Effect of E4 on TNF-α Expression in Endometriotic-like Lesions and Peritoneal Fluid
3.6. Effect of E4 on Esr2, Esr1, and Pgr mRNA Expression in Endometriotic-like Lesions
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | GenBank Access Number | Primer Sequences (5′–3′) | Amplicon (bp) |
---|---|---|---|
Esr1 | NM_001302532.1 | F: CTTGGAAGGCCGAAATGAAATG R: GGCAGGGCTATTCTTCTTAGTG | 103 |
Esr2 | NM_207707.1 | F: CTGGGTATCATTACGGTGTCTG R: GATTCGTGGCTGGACAGATATAG | 99 |
Pgr | NM_008829.2 | F: CCTGACACTTCCAGCTCTTT R: CGGAAACCTGGCAGAGATTTA | 100 |
Tnf | NM_001278601.1 | F: CTACCTTGTTGCCTCCTCTTT R: GAGCAGAGGTTCAGTGATGTAG | 116 |
Rn18s | NR_003278.3 | F: CTGAGAAACGGCTACCACATC R: GCCTCGAAAGAGTCCTGTATTG | 107 |
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Zabala, A.S.; Conforti, R.A.; Delsouc, M.B.; Filippa, V.; Montt-Guevara, M.M.; Giannini, A.; Simoncini, T.; Vallcaneras, S.S.; Casais, M. Estetrol Inhibits Endometriosis Development in an In Vivo Murine Model. Biomolecules 2024, 14, 580. https://doi.org/10.3390/biom14050580
Zabala AS, Conforti RA, Delsouc MB, Filippa V, Montt-Guevara MM, Giannini A, Simoncini T, Vallcaneras SS, Casais M. Estetrol Inhibits Endometriosis Development in an In Vivo Murine Model. Biomolecules. 2024; 14(5):580. https://doi.org/10.3390/biom14050580
Chicago/Turabian StyleZabala, Ana Sofia, Rocío Ayelem Conforti, María Belén Delsouc, Verónica Filippa, Maria Magdalena Montt-Guevara, Andrea Giannini, Tommaso Simoncini, Sandra Silvina Vallcaneras, and Marilina Casais. 2024. "Estetrol Inhibits Endometriosis Development in an In Vivo Murine Model" Biomolecules 14, no. 5: 580. https://doi.org/10.3390/biom14050580