SUMOylation in Giardia lamblia: A Conserved Post-Translational Modification in One of the Earliest Divergent Eukaryotes
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of SUMO in Giardia lamblia
2.2. Identification of the SUMOylation Pathway Components
3. Experimental Section
3.1. Parasites, Cells and Media
3.2. Mice
3.3. Bioinformatics Analysis
3.4. Isolation of gsumo; gsp; gsae2; gubc9
3.5. Plasmid Construction by gSUMO Over-expression
3.6. Recombinant Protein gSUMO Fused to GST Expression and Purification
3.7. Monoclonal Antibody Production
3.8. Western Blotting
3.9. Immunofluorescence Assay
4. Conclusions
Acknowledgments
Author Contribution
Conflict of Insterest
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Vranych, C.V.; Merino, M.C.; Zamponi, N.; Touz, M.C.; Rópolo, A.S. SUMOylation in Giardia lamblia: A Conserved Post-Translational Modification in One of the Earliest Divergent Eukaryotes. Biomolecules 2012, 2, 312-330. https://doi.org/10.3390/biom2030312
Vranych CV, Merino MC, Zamponi N, Touz MC, Rópolo AS. SUMOylation in Giardia lamblia: A Conserved Post-Translational Modification in One of the Earliest Divergent Eukaryotes. Biomolecules. 2012; 2(3):312-330. https://doi.org/10.3390/biom2030312
Chicago/Turabian StyleVranych, Cecilia V., María C. Merino, Nahuel Zamponi, María C. Touz, and Andrea S. Rópolo. 2012. "SUMOylation in Giardia lamblia: A Conserved Post-Translational Modification in One of the Earliest Divergent Eukaryotes" Biomolecules 2, no. 3: 312-330. https://doi.org/10.3390/biom2030312