Next Article in Journal
Protection of the Queuosine Biosynthesis Enzyme QueF from Irreversible Oxidation by a Conserved Intramolecular Disulfide
Previous Article in Journal
Biosynthesis of Sulfur-Containing tRNA Modifications: A Comparison of Bacterial, Archaeal, and Eukaryotic Pathways
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 7
Issue 1
10.3390/biom7010028
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Evaluation of Stability of Amylose Inclusion Complexes Depending on Guest Polymers and Their Application to Supramolecular Polymeric Materials

by
Tomonari Tanaka
1,*,
Atsushi Tsutsui
1,
Kazuya Tanaka
2,
Kazuya Yamamoto
2 and
Jun-ichi Kadokawa
2,*
1
Department of Biobased Materials Science, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
2
Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University, 1-21-40 Korimoto, Kagoshima 860-0065, Japan
*
Authors to whom correspondence should be addressed.
Biomolecules 2017, 7(1), 28; https://doi.org/10.3390/biom7010028
Submission received: 20 February 2017 / Revised: 9 March 2017 / Accepted: 9 March 2017 / Published: 15 March 2017
Browse Figures
Versions Notes

Abstract

:
This paper describes the evaluation of the stability of amylose–polymer inclusion complexes under solution state in dimethyl sulfoxide (DMSO) depending on guest polymers. The three complexes were prepared by the vine-twining polymerization method using polytetrahydrofuran (PTHF), poly(ε-caprolactone) (PCL), and poly(l-lactide) (PLLA) as guest polymers. The stability investigation was conducted at desired temperatures (25, 30, 40, 60 °C) in DMSO solutions of the complexes. Consequently, the amylose–PTHF inclusion complex was dissociated at 25 °C, while the other complexes were stable under the same conditions. When the temperatures were elevated, the amylose–PCL and amylose–PLLA complexes were dissociated at 40 and 60 °C, respectively. We also found that amylose inclusion supramolecular polymers which were prepared by the vine-twining polymerization using primer-guest conjugates formed films by the acetylation of amylose segments. The film from acetylated amylose–PLLA supramolecular polymer had higher storage modulus than that from acetylated amylose–PTHF supramolecular polymer, as a function of temperature.

1. Introduction

Amylose is an abundant natural polysaccharide as an energy resource present in starch [1]. Besides this vital role in nature, it is a well-known functional polymer and acts as a host molecule to form supramolecular inclusion complexes with various hydrophobic guest molecules, owing to its left-handed helical conformation and hydrophobic nature inside the cavity [2]. However, a limitation of binding directly toward polymeric guest molecules into the cavity has been found, because the driving force for complexation is implied by weak hydrophobic interaction [3,4,5,6,7,8,9,10,11,12]. We have developed an efficient method for the formation of inclusion complexes from amylose and polymeric guest molecules by means of enzymatic polymerization [13,14,15,16,17,18,19,20].
Amylose with well-defined structure is synthesized by enzymatic polymerization catalyzed by phosphorylase [21,22,23,24], as the enzymatic approach has been well accepted as a powerful tool to obtain polysaccharides with precisely controlled structure [16,20,25]. The phosphorylase-catalyzed enzymatic polymerization occurs by using α-d-glucose 1-phoshate (G-1-P) as a monomer and maltooligosaccharide as a primer according to the following reversible reaction: (α(1→4)-G)n + G-1-P ⇄ (α(1→4)-G)n+1 + P. In the reaction, a glucose (G) residue is transferred from G-1-P to the non-reducing 4-OH propagating terminus of a α(1→4)-glucan chain, liberating inorganic phosphate (P). We have found that inclusion complexes are gradually formed with the progress of propagation when the phosphorylase-catalyzed enzymatic polymerization was carried out in the presence of hydrophobic guest polymers dispersed in an aqueous buffer solvent (Figure 1a) [13,14,15,16,17,18,19,20]. The process of propagation from the shorter α(1→4)-glucan chain (maltooligosaccharide primer) to the longer α(1→4)-glucan chain (amylose) efficiently induces complexation with polymeric guest molecules. As the process of such complexation is similar to the way that vines of plants grow twining around a rod, the authors have named this polymerization approach “vine-twining polymerization”. In previous studies, hydrophobic polyethers such as polytetrahydrofuran (PTHF) [26,27] and hydrophobic polyesters such as poly(ε-caprolactone) (PCL) and poly(l-lactide) (PLLA) [28,29,30] have been found to form inclusion complexes with amylose by the vine-twining polymerization (Figure 1a).
By means of the vine-twining polymerization method, we have achieved the synthesis of inclusion supramolecular polymers which are composed of a continuum of the amylose–PTHF and amylose–PLLA inclusion complexes [31,32,33,34]. When primer (maltoheptaose, G7)-guest conjugate substrates (G7–PTHF and G7–PLLA) were used in the vine-twining polymerization, where the termini of the guest polymers were covalently connected with the reducing end of G7 via a triazole ring (Figure 2a), a propagating amylose chain which was started from the G7 segment in the G7-guest conjugate by the phosphorylase catalysis included the guest segment in the other substrate. This special type of the vine-twining polymerization among the substrates successively takes place, giving rise to the inclusion supramolecular polymers composed of amylose and guest polymer PTHF or PLLA.
To understand the natures of amylose–polymer inclusion complexes further, in this study we investigated the evaluation of their stability under solution state in dimethyl sulfoxide (DMSO) depending on guest polymers. On the basis of the findings, we also evaluated the mechanical properties of amylose triacetate (ATA)–PTHF and ATA–PLLA supramolecular polymeric films as a function of temperature, which were found to be obtained by acetylation of the original amylose inclusion supramolecular polymers. In a previous study, we also prepared a supramolecular film via the formation of a hydrogel by the vine-twining polymerization approach using the appropriately designed graft copolymer (i.e., carboxymethyl cellulose-graft-PCL) [35]. In this system, the enzymatically produced amyloses formed inclusion complexes with grafted PCL chains in the intermolecular graft copolymers, which acted as cross-linking points, resulting in network structures. Such network structures contributed to forming the hydrogel from the vine-twining polymerization solution, which was easily converted into the supramolecular film by drying. The present study provides a new type of supramolecular film constructed from linear polymeric structures, which are based on a completely different preparative strategy from that of the previous study.

2. Results and Discussion

2.1. Evaluation of the Stability of Inclusion Complexes Under Solution State

The amylose–polymer inclusion complexes were prepared by vine-twining polymerization using PTHF (Number-average molecular weight (Mn) = 2800 by 1H nuclear magnetic resonance (NMR)), PCL (Mn = 2400 by 1H NMR), and PLLA (Mn = 2600 by 1H NMR) according to literature procedures (Figure 1a) [27,29,30]. The structures of the inclusion complexes were supported by the 1H NMR spectra (see Materials and Methods section). The X-ray diffraction (XRD) patterns of the products were identical with those of the literature data; 2θ = ca. 13 and 20° for 61-helix from PTHF and PCL, 2θ = ca. 12 and 19° for 71-helix from PLLA (Figure 3a,c,g).
As the inclusion complexes were soluble in DMSO, the stability investigations under solution state were conducted in DMSO. After the inclusion complexes were treated in DMSO for 12 h at desired temperatures, the products were separated into chloroform-soluble and -insoluble fractions (Figure 1b). The XRD pattern of the chloroform-insoluble fraction after the treatment of the amylose–PTHF inclusion complex at 25 °C was broadened, which is completely different from that before treatment (Figure 3a,b). On the other hand, the XRD patterns of the chloroform-insoluble fractions from the other complexes remained intact by treatment at 25 °C (Figure 3c,d,g,h). These results indicate that the dissociation of the amylose–PTHF inclusion complex probably occurred, while the amylose–PCL and –PLLA inclusion complexes were stable at that temperature. Indeed, the 1H NMR spectrum of the chloroform-insoluble fraction (0.5 M NaOD/D2O) from the amylose–PTHF inclusion complex shows signals assignable to amylose, but does not show signals due to PTHF (Figure 4a). Furthermore, the 1H NMR spectrum of the chloroform-soluble fraction (CDCl3) from the same complex supports the structure of PTHF (Figure 5a).
By elevating the treatment temperature of the amylose–PCL inclusion complex to 30 °C, the XRD pattern of the chloroform-insoluble fraction was not significantly changed from the original pattern (Figure 3e). Moreover, the 1H NMR spectrum of the same sample (0.5 M NaOD/D2O) showed signals assignable to both amylose and 6-hydroxycaproic acid sodium salt (the hydrolyzed product from PCL under alkaline condition in 0.5 M NaOD/D2O) (Figure 4b), supporting that the inclusion complex retains stability after treatment at that temperature. When the treatment temperature of this inclusion complex was elevated to 40 °C, the XRD pattern of the chloroform-insoluble fraction was broadened (Figure 3f) as same as that treated from the amylose–PTHF inclusion complex at 25 °C. The 1H NMR spectrum of this fraction (0.5 M NaOD/D2O) only showed a signal assignable to amylose (Figure 4c), whereas the 1H NMR spectrum of the chloroform-soluble fraction (CDCl3) supports the structure of PCL (Figure 5b). These results suggest that the amylose–PCL inclusion complex was dissociated by treatment in DMSO at 40 °C.
When the treatment temperatures of the amylose–PLLA inclusion complex were elevated up to 40 °C, the XRD patterns of the chloroform-insoluble fractions were not significantly changed from the original data (Figure 3h–j). Furthermore, in the 1H NMR spectrum of the chloroform-insoluble fraction by the treatment at 40 °C in 0.5 M NaOD/D2O (Figure 4d), the signals due to both amylose and l-lactic acid sodium salt (the hydrolyzed product from PLLA under alkaline condition in 0.5 M NaOD/D2O) are detected. By further elevating the treatment temperature of the amylose–PLLA inclusion complex to 60 °C, the XRD pattern of the chloroform-insoluble fraction is broadened, which is completely different from that before treatment (Figure 3h,k). Indeed, the signals assignable to amylose are only detected in the 1H NMR spectrum of this fraction (0.5 M NaOD/D2O) (Figure 4e). Furthermore, the 1H NMR spectrum of the chloroform-soluble fraction (CDCl3) supports the structure of PLLA (Figure 5c). These results indicate the occurrence of the dissociation of the amylose–PLLA inclusion complex by treatment in DMSO at 60 °C.
Based on the above results, we conclude the following order for the stability of the inclusion complexes under solution state in DMSO: amylose–PLLA inclusion complex > amylose–PCL inclusion complex > amylose–PTHF inclusion complex. The difference in stability of the complexes is probably owing to the bulkiness of the guest polymers. The slenderest structure of PTHF induces the easier dissociation of the complex. With increasing the bulkiness by incorporating carbonyl groups into the PCL and PLLA main-chains and further methyl group into the PLLA main-chain, the guest polymers slip out from cavity of amylose less easily, leading to an enhancement of the stability of the complex in DMSO.

2.2. Preparation and Mechanical Properties of ATA Supramolecular Polymeric Films

Based on the above results, we also investigated the evaluation of the mechanical properties of ATA–PTHF and –PLLA supramolecular polymeric materials as a function of temperature. We found that the supramolecular polymeric films were obtained by acetylation of amylose segments, which were suitably subjected to dynamic mechanical analysis (DMA) measurement for the evaluation. The ATA supramolecular polymers were prepared from amylose–PTHF and –PLLA inclusion supramolecular polymers which were synthesized by the vine-twining polymerization using G7–PTHF and G7–PLLA, respectively [31,33], by the acetylation reaction using acetic anhydride (Ac2O) in pyridine in the presence of N,N-dimethyl-4-aminopyridine (DMAP) (Figure 2b). The 1H NMR spectra of the acetylated products showed signals assignable to the methyl of introduced acetyl groups, the acetylated amylose, and the guest polymer segments (Figure 6). The degrees of substitution on hydroxy groups by acetyl groups were determined by the 1H NMR spectra to be quantitative in both the supramolecular polymers. Figure 7 shows the gel permeation chromatography (GPC) traces of acetylated products, which show peaks with unimodal profiles. The Mn values of ATA–PTHF and –PLLA supramolecular products were calculated to be 1.3 × 105 g·mol−1 (Weight-average molecular weight (Mw)/Mn = 3.6) and 5.4 × 105 g·mol−1 (Mw/Mn = 5.2), respectively. The Mn values of primer–guest conjugate substrates (G7–PTHF and G7–PLLA) used for the vine-twining polymerization were much smaller (3.3 × 103 g·mol−1 and 2.8 × 103 g·mol−1, respectively), suggesting that the inclusion complexation by amylose in the supramolecular polymers was not dissociated under the acetylation condition.
We found that the present ATA inclusion supramolecular polymers were soluble in common organic solvents and could easily form films, although the amylose–polymer supramolecular polymers before acetylation were mostly insoluble in such organic solvents. Accordingly, flexible cast films were obtained from chloroform solutions of the ATA–PTHF and –PLLA supramolecular products. Figure 8 shows the DMA measurements of the resulting films from ATA supramolecular polymers and potato ATA (Mn = 1.8 × 105 g·mol−1, Mw/Mn = 7.2 by GPC), which was prepared by the same acetylation procedure from a commercial potato amylose sample. Storage moduli (E′) of the ATA–PLLA supramolecular polymeric film were higher than those of the ATA–PTHF supramolecular polymeric film and potato ATA on the whole temperature range. The descent curve of E′ of the ATA–PLLA supramolecular polymeric film shifted to the higher temperature region compared with that of the ATA–PTHF supramolecular polymeric film and potato ATA (Figure 8a), suggesting that PLLA segments included by amylose had enhanced the stability of the supramolecular polymeric film under heating. E′ of the ATA–PTHF supramolecular polymeric film dropped off at 150 °C, suggesting that the inclusion by amylose might be dissociated in the ATA–PTHF supramolecular polymeric film under heating. There is no such behavior in the case of the ATA–PLLA supramolecular polymeric film. Furthermore, loss moduli (E′′) of the ATA–PLLA supramolecular polymeric film are shown to be higher than those of the ATA–PTHF supramolecular polymeric film at 150–165 °C (Figure 8b). These DMA results support that the film from ATA–PLLA supramolecular polymer is more stable at high temperature than that from ATA–PTHF supramolecular polymer and potato ATA.

3. Materials and Methods

3.1. Materials

The primer G7 was prepared by selective cleavage of one glucosidic bond of β-cyclodextrin under acidic conditions [36]. The guest polymers PTHF, PCL, and PLLA were synthesized by ring-opening polymerization of tetrahydrofuran, ε-caprolactone, and l-lactide monomers initiated with methyl trifluoromethanesulfonate, 6-hydroxycaproic acid, and l-lactic acid, respectively [37,38,39]. Amylose–PTHF and –PLLA inclusion supramolecular polymers were synthesized according to the literature procedures [31,33]. Thermostable phosphorylase from Aquifex aeolicus VF5 was supplied from Ezaki Glico Co. Ltd. (Osaka, Japan) [23,40,41]. Potato amylose was purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO, USA). Other reagents and solvents were commercially available and used without further purification.

3.2. Preparation of Amylose–Polymer Inclusion Complexes

A typical experimental procedure was as follows. A mixture of the guest polymer (50 mg) with sodium acetate buffer (pH 6.2, 0.2 mol/L, 4.0 mL) was sonicated to obtain a dispersion. After the addition of G7 (1.2 mg, 1.0 mmol), G-1-P disodium salt (76.1 mg, 0.25 mmol), and thermostable phosphorylase (9 U) to the suspension, the mixture was stirred vigorously for 15 h at 40–45 °C. The precipitate was collected by filtration, washed with water, acetone, and chloroform, and then dried under reduced pressure at room temperature to yield the inclusion complex. 1H NMR spectra (DMSO-d6 + D2O): amylose–PTHF inclusion complex; δ 1.48–1.55 (br, −C−CH2−CH2−C− of PTHF), 3.15–3.66 (m, H2−H6 of amylose, overlapping with HOD), 3.30–3.37 (br, O−CH2−C−C−CH2− of PTHF), 5.11 (br, H1 of amylose); amylose–PCL inclusion complex; δ 1.33–1.36 (br, O=C−C−C−CH2 of PCL), 1.55–1.59 (br, O=C−C−CH2−C−CH2 of PCL), 2.19–2.20 (br, O=C−CH2 of PCL), 3.15–3.66 (m, H2−H6 of amylose, overlapping with HOD), 3.58–3.60 (br, C−CH2−OH of PCL (terminus)), 4.02–4.22 (br, C−CH2−O− of PCL), 5.11 (br, H1 of amylose); amylose–PLLA inclusion complex; δ 1.45–1.48 (m, CH3 of PLLA), 3.15–3.66 (m, H2−H6 of amylose, overlapping with HOD), 5.11 (br, H1 of amylose), 5.20–5.22 (br, CH of PLLA).

3.3. Evaluation of the Stability of Inclusion Complexes

Mixtures of the aforementioned inclusion complexes (60 mg) with DMSO (4 mL) were stirred for 12 h at desired temperatures (25, 30, 40, 60 °C). Chloroform was then added to the mixtures to obtain precipitates. The precipitates were isolated by filtration and dried under reduced pressure for 3 h at room temperature. The filtrates were concentrated by evaporation and dried under reduced pressure for 5 h at 110 °C. The resulting two fractions were then characterized.

3.4. Preparation of ATA Inclusion Supramolecular Polymeric Films

A typical experimental procedure was as follows. Ac2O (0.27 mL, 2.86 mmol) was dropped to a mixture of the amylose–polymer inclusion supramolecular polymer (30 mg), DMAP (2 mg, 0.016 mmol), and pyridine (0.5 mL), followed by stirring for 3 days at room temperature. The reaction mixture was diluted with dichloromethane, and extracted with saturated sodium dicarbonate aqueous solution, 1 M hydrochloride aqueous solution, and brine. The organic layer was dried with sodium sulfate, and the filtrate was dried under reduced pressure to yield the ATA inclusion supramolecular polymer. 1H NMR spectra (CDCl3): ATA–PTHF supramolecular polymer; δ 1.55 (br, –C–CH2–CH2–C– of PTHF), 1.91–2.15 (m, CH3 of Ac), 3.34 (br, OCH3), 3.42 (br, O–CH2–C–C–CH2–O of PTHF), 3.89, 4.20, 4.52, 4.67, 5.24, and 5.33 (amylose); ATA–PLLA supramolecular polymer; δ 1.59 (br, CH3 of PLLA), 1.98–2.21 (m, CH3 of Ac), 3.95, 4.27, 4.55, 4.72, 5.30, and 5.39 (amylose), 5.16 (q, CH of PLLA). The films were prepared by casting chloroform solutions (33 mg/mL) of the resulting ATA inclusion supramolecular polymers on a glass plate, and dried at room temperature.

3.5. Measurements

The 1H NMR spectra were recorded using a ECX400 (JEOL, Akishima, Tokyo, Japan), ECA600 (JEOL), and a BioSpin AV-300 spectrometer (Bruker, Yokohama, Kanagawa, Japan). The powder XRD measurements were performed using a X’Pert Pro MPD diffractometer (PANalytical B.V., Almelo, The Netherlands) with Ni-filtered Cu Kα radiation (λ = 0.15418 nm). GPC measurements were conducted using a system consisting of a LC-20AD pump (Shimadzu, Kyoto, Japan), a CTO-10A column oven, an RID-10A refractive index detector, and two TSK gel Super HZM-N (4.6 × 150 mm; TOSOH, Tokyo, Japan) columns. Chloroform was used as the eluent at a flow rate of 0.25 mL/min at 40 °C. Polystyrene samples were used as standards. The DMA were performed using a DMA 2980 Dynamic Mechanical Analyzer (TA Instruments, Tokyo, Japan) at heating rate of 5 °C/min at 1 Hz using a supramolecular polymeric film (5 mm × 20 mm).

4. Conclusions

In this paper, we reported the evaluation of the stability of amylose–polymer inclusion complexes under solution state in DMSO. The analytical results of the products indicated that stability in the DMSO solutions increased in accordance with the bulkiness of the guest polymers as follows: PTHF < PCL < PLLA. Furthermore, we achieved preparation of ATA inclusion supramolecular films from the vine-twining supramolecular polymeric products by acetylation of amylose segments. The DMA analysis indicated that the dynamic moduli of the film from ATA inclusion supramolecular polymer depended on the type of included guest polymers.

Acknowledgments

We acknowledge the supplement of thermostable phosphorylase from Ezaki Glico Co. Ltd, Osaka, Japan. This work was financially supported by a research grant from The Mazda Foundation (No. 14KK-122).

Author Contributions

Tomonari Tanaka, Kazuya Yamamoto, and Jun-ichi Kadokawa conceived the project, designed the experiments, directed the research, and wrote the manuscript. Atsushi Tsutsui and Kazuya Tanaka performed the experiments. All authors discussed the results and edited the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Schuerch, C. Polysaccharides. In Encyclopedia of Polymer Science and Engineering, 2nd ed.; Mark, H.F., Bilkales, N., Overberger, C.G., Eds.; John Wiley & Sons: New York, NY, USA, 1986; Volume 13, pp. 87–162. [Google Scholar]
  2. Putseys, J.A.; Lamberts, L.; Delcour, J.A. Amylose-inclusion complexes: Formation, identity and physico-chemical properties. J. Cereal Sci. 2010, 51, 238–247. [Google Scholar] [CrossRef]
  3. Shogren, R.L.; Greene, R.V.; Wu, Y.V. Complexes of starch polysaccharides and poly(ethylene-co-acrylic acid): Structure and stability in solution. J. Appl. Polym. Sci. 1991, 42, 1701–1709. [Google Scholar] [CrossRef]
  4. Shogren, R.L. Complexes of starch with telechelic poly(e-caprolactone) phosphate. Carbohydr. Polym. 1993, 22, 93–98. [Google Scholar] [CrossRef]
  5. Star, A.; Steuerman, D.W.; Heath, J.R.; Stoddart, J.F. Starched carbon nanotubes. Angew. Chem. Int. Ed. 2002, 41, 2508–2512. [Google Scholar] [CrossRef]
  6. Ikeda, M.; Furusho, Y.; Okoshi, K.; Tanahara, S.; Maeda, K.; Nishino, S.; Mori, T.; Yashima, E. A luminescent poly(phenylenevinylene)-amylose composite with supramolecular liquid crystallinity. Angew. Chem. Int. Ed. 2006, 45, 6491–6495. [Google Scholar] [CrossRef] [PubMed]
  7. Kida, T.; Minabe, T.; Okabe, S.; Akashi, M. Partially-methylated amyloses as effective hosts for inclusion complex formation with polymeric guests. Chem. Commun. 2007, 1559–1561. [Google Scholar] [CrossRef] [PubMed]
  8. Kaneko, Y.; Kyutoku, T.; Shimomura, N.; Kadokawa, J. Formation of amylose-poly(tetrahydrofuran) inclusion complexes in ionic liquid media. Chem. Lett. 2011, 40, 31–33. [Google Scholar] [CrossRef]
  9. Rachmawati, R.; Woortman, A.J.J.; Loos, K. Facile preparation method for inclusion complexes between amylose and polytetrahydrofurans. Biomacromolecules 2013, 14, 575–583. [Google Scholar] [CrossRef] [PubMed]
  10. Kumar, K.; Woortman, A.J.J.; Loos, K. Synthesis of amylose-polystyrene inclusion complexes by a facile preparation route. Biomacromolecules 2013, 14, 1955–1960. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  11. Rachmawati, R.; Woortman, A.J.J.; Loos, K. Tunable properties of inclusion complexes between amylose and polytetrahydrofuran. Macromol. Biosci. 2013, 13, 767–776. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  12. Rachmawati, R.; Woortman, A.J.J.; Loos, K. Solvent-responsive behavior of inclusion complexes between amylose and polytetrahydrofuran. Macromol. Biosci. 2014, 14, 56–68. [Google Scholar] [CrossRef] [PubMed]
  13. Kaneko, Y.; Kadokawa, J. Vine-twining polymerization: A new preparation method for well-defined supramolecules composed of amylose and synthetic polymers. Chem. Rec. 2005, 5, 36–46. [Google Scholar] [CrossRef] [PubMed]
  14. Kaneko, Y.; Kadokawa, J. Synthesis of nanostructured bio-related materials by hybridization of synthetic polymers with polysaccharides or saccharide residues. J. Biomater. Sci. Polym. Ed. 2006, 17, 1269–1284. [Google Scholar] [CrossRef] [PubMed]
  15. Kaneko, Y.; Kadokawa, J. Preparation of Polymers with Well-Defined Nanostructure in the Polymerization Field. In Modern Trends in Macromolecular Chemistry; Lee, J.N., Ed.; Nova Science Publishers Inc.: Hauppauge, NY, USA, 2009; pp. 199–217. [Google Scholar]
  16. Kadokawa, J. Precision polysaccharide synthesis catalyzed by enzymes. Chem. Rev. 2011, 111, 4308–4345. [Google Scholar] [CrossRef] [PubMed]
  17. Kadokawa, J. Preparation and applications of amylose supramolecules by means of phosphorylase-catalyzed enzymatic polymerization. Polymers 2012, 4, 116–133. [Google Scholar] [CrossRef]
  18. Kadokawa, J. Architecture of amylose supramolecules in form of inclusion complexes by phosphorylase-catalyzed enzymatic polymerization. Biomolecules 2013, 3, 369–385. [Google Scholar] [CrossRef] [PubMed]
  19. Kadokawa, J. Chemoenzymatic synthesis of functional amylosic materials. Pure Appl. Chem. 2014, 86, 701–709. [Google Scholar] [CrossRef]
  20. Shoda, S.; Uyama, H.; Kadokawa, J.; Kimura, S.; Kobayashi, S. Enzymes as green catalysts for precision macromolecular synthesis. Chem. Rev. 2016, 116, 2307–2413. [Google Scholar] [CrossRef] [PubMed]
  21. Ziegast, G.; Pfannemüller, B. Linear and star-shaped hybrid polymers. Phosphorolytic syntheses with di-functional, oligo-functional and multifunctional primers. Carbohydr. Res. 1987, 160, 185–204. [Google Scholar] [CrossRef]
  22. Kitaoka, M.; Hayashi, K. Carbohydrate-processing phosphorolytic enzymes. Trends Glycosci. Glycotechnol. 2002, 14, 35–50. [Google Scholar] [CrossRef]
  23. Yanase, M.; Takaha, T.; Kuriki, T. α-Glucan phosphorylase and its use in carbohydrate engineering. J. Sci. Food Agric. 2006, 86, 1631–1635. [Google Scholar] [CrossRef]
  24. Ohdan, K.; Fujii, K.; Yanase, M.; Takaha, T.; Kuriki, T. Enzymatic synthesis of amylose. Biocatal. Biotransform. 2006, 24, 77–81. [Google Scholar] [CrossRef]
  25. Kadokawa, J. Precision synthesis of functional polysaccharide materials by phosphorylase-catalyzed enzymatic reactions. Polymers 2016, 8, 138. [Google Scholar] [CrossRef]
  26. Kadokawa, J.; Kaneko, Y.; Tagaya, H.; Chiba, K. Synthesis of an amylose-polymer inclusion complex by enzymatic polymerization of glucose 1-phosphate catalyzed by phosphorylase enzyme in the presence of polyTHF: A new method for synthesis of polymer–polymer inclusion complexes. Chem. Commun. 2001, 449–450. [Google Scholar] [CrossRef]
  27. Kadokawa, J.; Kaneko, Y.; Nagase, S.; Takahashi, T.; Tagaya, H. Vine-twining polymerization: Amylose twines around polyethers to form amylose-Polyether inclusion complexes. Chem. Eur. J. 2002, 8, 3321–3326. [Google Scholar] [CrossRef]
  28. Kadokawa, J.; Kaneko, Y.; Nakaya, A.; Tagaya, H. Formation of an amylose-polyester inclusion complex by means of phosphorylase-catalyzed enzymatic polymerization of α-d-glucose 1-phosphate monomer in the presence of poly(e-caprolactone). Macromolecules 2001, 34, 6536–6538. [Google Scholar] [CrossRef]
  29. Kadokawa, J.; Nakaya, A.; Kaneko, Y.; Tagaya, H. Preparation of inclusion complexes between amylose and ester-containing polymers by means of vine-twining polymerization. Macromol. Chem. Phys. 2003, 204, 1451–1457. [Google Scholar] [CrossRef]
  30. Kaneko, Y.; Ueno, K.; Yui, T.; Nakahara, K.; Kadokawa, J. Amylose’s recognition of chirality in polylactides on formation of inclusion complexes in vine-twining polymerization. Macromol. Biosci. 2011, 11, 1407–1415. [Google Scholar] [CrossRef] [PubMed]
  31. Tanaka, T.; Sasayama, S.; Nomura, S.; Yamamoto, K.; Kimura, Y.; Kadokawa, J. An amylose-poly(l-lactide) inclusion supramolecular polymer: Enzymatic synthesis by means of vine-twining polymerization using a primer-guest conjugate. Macromol. Chem. Phys. 2013, 214, 2829–2834. [Google Scholar] [CrossRef]
  32. Tanaka, T.; Sasayama, S.; Yamamoto, K.; Kimura, Y.; Kadokawa, J. Evaluating relative chain orientation of amylose and poly(l-lactide) in inclusion complexes formed by vine-twining polymerization using primer-guest conjugates. Macromol. Chem. Phys. 2015, 216, 794–800. [Google Scholar] [CrossRef]
  33. Tanaka, T.; Tsutsui, A.; Gotanda, R.; Sasayama, S.; Yamamoto, K.; Kadokawa, J. Synthesis of amylose-polyether inclusion supramolecular polymers by vine-twining polymerization using maltoheptaose-functionalized poly(tetrahydrofuran) as a primer-guest conjugate. J. Appl. Glycosci. 2015, 62, 135–141. [Google Scholar] [CrossRef]
  34. Tanaka, T.; Gotanda, R.; Tsutsui, A.; Sasayama, S.; Yamamoto, K.; Kimura, Y.; Kadokawa, J. Synthesis and gel formation of hyperbranched supramolecular polymer by vine-twining polymerization using branched primer-guest conjugate. Polymer 2015, 73, 9–16. [Google Scholar] [CrossRef]
  35. Kadokawa, J.; Nomura, S.; Hatanaka, D.; Yamamoto, K. Preparation of polysaccharide supramolecular films by vine-twining polymerization approach. Carbohydr. Polym. 2013, 98, 611–617. [Google Scholar] [CrossRef] [PubMed]
  36. Von Braunmühl, V.; Jonas, G.; Stadler, R. Enzymatic grafting of amylose from poly(dimethylsiloxanes). Macromolecules 1995, 28, 17–24. [Google Scholar] [CrossRef]
  37. Smith, S.; Hubin, A.J. The preparation and chemistry of dicationically active polymers of tetrahydrofuran. J. Macromol. Sci. A Chem. 1973, 7, 1399–1413. [Google Scholar] [CrossRef]
  38. Nomura, N.; Taira, A.; Tomioka, T.; Okada, M. A catalytic approach for cationic living polymerization: Sc(OTf)3-catalyzed ring-opening polymerization of lactones. Macromolecules 2000, 33, 1497–1499. [Google Scholar] [CrossRef]
  39. Fan, Y.J.; Chen, G.P.; Tanaka, J.; Tateishi, T. l-Phe end-capped poly(l-lactide) as macroinitiator for the synthesis of poly(l-lactide)-b-poly(l-lysine) block copolymer. Biomacromolecules 2005, 6, 3051–3056. [Google Scholar] [CrossRef] [PubMed]
  40. Bhuiyan, S.H.; Rus’d, A.A.; Kitaoka, M.; Hayashi, K. Characterization of a hyperthermostable glycogen phosphorylase from Aquifex aeolicus expressed in Escherichia coli. J. Mol. Catal. B Enzym. 2003, 22, 173–180. [Google Scholar] [CrossRef]
  41. Yanase, M.; Takata, H.; Fujii, K.; Takaha, T.; Kuriki, T. Cumulative effect of amino acid replacements results in enhanced thermostability of potato type L α-glucan phosphorylase. Appl. Environ. Microbiol. 2005, 71, 5433–5439. [Google Scholar] [CrossRef] [PubMed]
Biomolecules 07 00028 g001 550
Figure 1. Preparation of: (a) inclusion complexes by vine-twining polymerization using polytetrahydrofuran (PTHF), poly(ε-caprolactone) (PCL), and poly(l-lactide) (PLLA) as guest polymers and (b) their dissociation under solution state in dimethyl sulfoxide (DMSO).
Figure 1. Preparation of: (a) inclusion complexes by vine-twining polymerization using polytetrahydrofuran (PTHF), poly(ε-caprolactone) (PCL), and poly(l-lactide) (PLLA) as guest polymers and (b) their dissociation under solution state in dimethyl sulfoxide (DMSO).
Biomolecules 07 00028 g001
Biomolecules 07 00028 g002 550
Figure 2. Preparation of: (a) amylose triacetate (ATA) inclusion supramolecular polymers and (b) their films. DMAP: N,N-dimethyl-4-aminopyridine.
Figure 2. Preparation of: (a) amylose triacetate (ATA) inclusion supramolecular polymers and (b) their films. DMAP: N,N-dimethyl-4-aminopyridine.
Biomolecules 07 00028 g002
Biomolecules 07 00028 g003 550
Figure 3. X-ray diffraction (XRD) profiles of: (a) amylose–PTHF inclusion complex; (b) chloroform-insoluble fraction by its treatment in DMSO at 25 °C; (c) amylose–PCL inclusion complex, chloroform-insoluble fractions by its treatment in DMSO at (d) 25 °C, (e) 30 °C, and (f) 40 °C; (g) amylose–PLLA inclusion complex, chloroform-insoluble fractions by its treatment in DMSO at (h) 25 °C, (i) 30 °C, (j) 40 °C, and (k) 60 °C.
Figure 3. X-ray diffraction (XRD) profiles of: (a) amylose–PTHF inclusion complex; (b) chloroform-insoluble fraction by its treatment in DMSO at 25 °C; (c) amylose–PCL inclusion complex, chloroform-insoluble fractions by its treatment in DMSO at (d) 25 °C, (e) 30 °C, and (f) 40 °C; (g) amylose–PLLA inclusion complex, chloroform-insoluble fractions by its treatment in DMSO at (h) 25 °C, (i) 30 °C, (j) 40 °C, and (k) 60 °C.
Biomolecules 07 00028 g003
Biomolecules 07 00028 g004 550
Figure 4. 1H nuclear magnetic resonance (NMR) spectra of: (a) chloroform-insoluble fraction by treatment of amylose–PTHF inclusion complex in DMSO at 25 °C; chloroform-insoluble fractions by treatment of amylose–PCL inclusion complex in DMSO at (b) 30 °C and (c) 40 °C; and chloroform-insoluble fractions by treatment of amylose–PLLA inclusion complex in DMSO at (d) 40 °C and (e) 60 °C (0.5 M NaOD/D2O).
Figure 4. 1H nuclear magnetic resonance (NMR) spectra of: (a) chloroform-insoluble fraction by treatment of amylose–PTHF inclusion complex in DMSO at 25 °C; chloroform-insoluble fractions by treatment of amylose–PCL inclusion complex in DMSO at (b) 30 °C and (c) 40 °C; and chloroform-insoluble fractions by treatment of amylose–PLLA inclusion complex in DMSO at (d) 40 °C and (e) 60 °C (0.5 M NaOD/D2O).
Biomolecules 07 00028 g004
Biomolecules 07 00028 g005 550
Figure 5. 1H NMR spectra of: (a) chloroform-soluble fraction by treatment of amylose–PTHF inclusion complex in DMSO at 25 °C; (b) chloroform-soluble fraction by treatment of amylose–PCL inclusion complex in DMSO at 40 °C; and (c) chloroform-soluble fraction by treatment of amylose–PLLA inclusion complex in DMSO at 60 °C (CDCl3).
Figure 5. 1H NMR spectra of: (a) chloroform-soluble fraction by treatment of amylose–PTHF inclusion complex in DMSO at 25 °C; (b) chloroform-soluble fraction by treatment of amylose–PCL inclusion complex in DMSO at 40 °C; and (c) chloroform-soluble fraction by treatment of amylose–PLLA inclusion complex in DMSO at 60 °C (CDCl3).
Biomolecules 07 00028 g005
Biomolecules 07 00028 g006 550
Figure 6. 1H NMR spectra of: (a) ATA–PTHF and (b) ATA–PLLA supramolecular polymers (CDCl3).
Figure 6. 1H NMR spectra of: (a) ATA–PTHF and (b) ATA–PLLA supramolecular polymers (CDCl3).
Biomolecules 07 00028 g006
Biomolecules 07 00028 g007 550
Figure 7. Gel permeation chromatography (GPC) traces of (a) ATA–PTHF and (b) ATA–PLLA supramolecular polymers.
Figure 7. Gel permeation chromatography (GPC) traces of (a) ATA–PTHF and (b) ATA–PLLA supramolecular polymers.
Biomolecules 07 00028 g007
Biomolecules 07 00028 g008 550
Figure 8. Dynamic mechanical analysis of (i) ATA–PTHF and (ii) ATA–PLLA supramolecular polymeric films, and (iii) potato ATA. (a) storage modulus (E′); (b) loss modulus (E′′).
Figure 8. Dynamic mechanical analysis of (i) ATA–PTHF and (ii) ATA–PLLA supramolecular polymeric films, and (iii) potato ATA. (a) storage modulus (E′); (b) loss modulus (E′′).
Biomolecules 07 00028 g008

Share and Cite

MDPI and ACS Style

Tanaka, T.; Tsutsui, A.; Tanaka, K.; Yamamoto, K.; Kadokawa, J.-i. Evaluation of Stability of Amylose Inclusion Complexes Depending on Guest Polymers and Their Application to Supramolecular Polymeric Materials. Biomolecules 2017, 7, 28. https://doi.org/10.3390/biom7010028

AMA Style

Tanaka T, Tsutsui A, Tanaka K, Yamamoto K, Kadokawa J-i. Evaluation of Stability of Amylose Inclusion Complexes Depending on Guest Polymers and Their Application to Supramolecular Polymeric Materials. Biomolecules. 2017; 7(1):28. https://doi.org/10.3390/biom7010028

Chicago/Turabian Style

Tanaka, Tomonari, Atsushi Tsutsui, Kazuya Tanaka, Kazuya Yamamoto, and Jun-ichi Kadokawa. 2017. "Evaluation of Stability of Amylose Inclusion Complexes Depending on Guest Polymers and Their Application to Supramolecular Polymeric Materials" Biomolecules 7, no. 1: 28. https://doi.org/10.3390/biom7010028

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop