Noncovalent Immobilization of Yarrowia lipolytica Lipase on Dendritic-Like Amino Acid-Functionalized Silica Nanoparticles
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Characterization
2.2. Lipase Production and Detection
2.3. Synthesis of SiO2/AA
2.4. Metal Ion Chelation on SiO2/AA
2.5. Lipase Immobilization
2.6. Reusability of Immobilized Lipase
3. Results
3.1. The Hydrolytic Activity of Immobilized Lipase
3.2. Reusability of SiO2/AA@YLIP2
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Fathi, Z.; Doustkhah, E.; Ebrahimipour, G.; Darvishi, F. Noncovalent Immobilization of Yarrowia lipolytica Lipase on Dendritic-Like Amino Acid-Functionalized Silica Nanoparticles. Biomolecules 2019, 9, 502. https://doi.org/10.3390/biom9090502
Fathi Z, Doustkhah E, Ebrahimipour G, Darvishi F. Noncovalent Immobilization of Yarrowia lipolytica Lipase on Dendritic-Like Amino Acid-Functionalized Silica Nanoparticles. Biomolecules. 2019; 9(9):502. https://doi.org/10.3390/biom9090502
Chicago/Turabian StyleFathi, Zahra, Esmail Doustkhah, Golamhossein Ebrahimipour, and Farshad Darvishi. 2019. "Noncovalent Immobilization of Yarrowia lipolytica Lipase on Dendritic-Like Amino Acid-Functionalized Silica Nanoparticles" Biomolecules 9, no. 9: 502. https://doi.org/10.3390/biom9090502