Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Growth Conditions and Stress Treatments
2.2. Production of Recombinant Proteins
2.3. RT-qPCR Analysis
2.4. Antibody Production and Western Blotting
2.5. Production and Characterization of Transgenic Arabidopsis Lines
2.6. Prediction of Conserved Motifs in the OsS1Fa1 Protein
2.7. Effect of MG132 on OsS1Fa1 Protein Level and Stability
3. Results
3.1. Expression of OsS1Fa1 Is Upregulated by Drought Stress
3.2. OsS1F1a Transcript and the Encoded Protein Exhibit Organ-Specific Expression
3.3. OsS1Fa1 Overexpression in Arabidopsis Enhances Drought Stress Tolerance
3.4. OsS1Fa1 Degradation Is Influenced by Proteasome Inhibition
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kim, S.-I.; Lee, K.H.; Kwak, J.S.; Kwon, D.H.; Song, J.T.; Seo, H.S. Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis. Plants 2021, 10, 2181. https://doi.org/10.3390/plants10102181
Kim S-I, Lee KH, Kwak JS, Kwon DH, Song JT, Seo HS. Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis. Plants. 2021; 10(10):2181. https://doi.org/10.3390/plants10102181
Chicago/Turabian StyleKim, Sung-Il, Kyu Ho Lee, Jun Soo Kwak, Dae Hwan Kwon, Jong Tae Song, and Hak Soo Seo. 2021. "Overexpression of Rice OsS1Fa1 Gene Confers Drought Tolerance in Arabidopsis" Plants 10, no. 10: 2181. https://doi.org/10.3390/plants10102181