Next Article in Journal
Diurnal Regulation of Leaf Water Status in High- and Low-Mannitol Olive Cultivars
Next Article in Special Issue
Light Signaling in Bud Outgrowth and Branching in Plants
Previous Article in Journal
Functions of Calcium-Dependent Protein Kinases in Plant Innate Immunity
Previous Article in Special Issue
Effects of Light and Wounding on Jasmonates in Rice phyAphyC Mutants
Article Menu

Export Article

Open AccessArticle
Plants 2014, 3(1), 177-195; doi:10.3390/plants3010177

Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings

1
Graduate School of Humanities and Sciences, Nara Women's University, Nara 630-8506, Japan
2
Faculty of Science, Nara Women's University, Nara 630-8506, Japan
3
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan
Present address: NIPPON Funmatsu Yakuhin CO., Ltd, Osaka 541-0045, Japan
Present address: Graduate School of Information Science, Nagoya University, Nagoya 464-8601, Japan
§
Present address: Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan;
*
Author to whom correspondence should be addressed.
Received: 15 November 2013 / Revised: 2 February 2014 / Accepted: 21 February 2014 / Published: 5 March 2014
(This article belongs to the Special Issue Plant Light Signalling)
View Full-Text   |   Download PDF [1230 KB, uploaded 5 March 2014]   |  

Abstract

Light regulates the expression and function of aquaporins, which are involved in water and solute transport. In Arabidopsis thaliana, mRNA levels of one of the aquaporin genes, TIP2;2, increase during dark adaptation and decrease under far-red light illumination, but the effects of light at the protein level and on the mechanism of light regulation remain unknown. Numerous studies have described the light regulation of aquaporin genes, but none have identified the regulatory mechanisms behind this regulation via specific photoreceptor signaling. In this paper, we focus on the role of phytochrome A (phyA) signaling in the regulation of the TIP2;2 protein. We generated Arabidopsis transgenic plants expressing a TIP2;2-GFP fusion protein driven by its own promoter, and showed several differences in TIP2;2 behavior between wild type and the phyA mutant. Fluorescence of TIP2;2-GFP protein in the endodermis of roots in the wild-type seedlings increased during dark adaptation, but not in the phyA mutant. The amount of the TIP2;2-GFP protein in wild-type seedlings decreased rapidly under far-red light illumination, and a delay in reduction of TIP2;2-GFP was observed in the phyA mutant. Our results imply that phyA, cooperating with other photoreceptors, modulates the level of TIP2;2 in Arabidopsis roots.
Keywords: Arabidopsis thaliana; aquaporin; dark adaptation; endodermis; light regulation; phytochrome; root; water transport Arabidopsis thaliana; aquaporin; dark adaptation; endodermis; light regulation; phytochrome; root; water transport
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Uenishi, Y.; Nakabayashi, Y.; Tsuchihira, A.; Takusagawa, M.; Hashimoto, K.; Maeshima, M.; Sato-Nara, K. Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings. Plants 2014, 3, 177-195.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top