Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of Active Compounds in BGSE
2.2. The Inhibitory Effects of Octadecanoic Acid Analogs on ATL Cell Lines
Compounds | IC50 (µM) | ||
---|---|---|---|
ED | Su9T01 | ||
α-ESA | (C18:3, n-5) | 8.9 | 29.3 |
γ-linolenic acid | (C18:3, n-6) | 61.3 | 174.3 |
α-linolenic acid | (C18:3, n-3) | 129.9 | 167.4 |
linoleic acid | (C18:2, n-6) | 100.7 | 180.1 |
oleic acid | (C18:1, n-9) | 500.0–166.7 | 500.0–166.7 |
elaidic acid | (C18:1, n-9) | >500.0 | >500.0 |
stearic acid | (C18:0) | 500.0–166.7 | >500.0 |
EGCG | (Positive Control) | 152.7 | 166.0 |
2.3. The Effect of α-ESA on ATL Cell Line and Phytohemagglutinin-Activated Human Peripheral Blood Mononuclear Cell (PBMC) Proliferation
3. Experimental
3.1. Chemicals
3.2. Identification of Compounds in BGSE
3.3. α-ESA Calibration Curve
3.4. ATL Cell Proliferation Assay
3.5. Isolation and Culture of PBMCs
3.6. Statistics
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Kai, H.; Akamatsu, E.; Torii, E.; Kodama, H.; Yukizaki, C.; Akagi, I.; Ino, H.; Sakakibara, Y.; Suiko, M.; Yamamoto, I.; et al. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants 2014, 3, 18-26. https://doi.org/10.3390/plants3010018
Kai H, Akamatsu E, Torii E, Kodama H, Yukizaki C, Akagi I, Ino H, Sakakibara Y, Suiko M, Yamamoto I, et al. Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds. Plants. 2014; 3(1):18-26. https://doi.org/10.3390/plants3010018
Chicago/Turabian StyleKai, Hisahiro, Ena Akamatsu, Eri Torii, Hiroko Kodama, Chizuko Yukizaki, Isao Akagi, Hisatoshi Ino, Yoichi Sakakibara, Masahito Suiko, Ikuo Yamamoto, and et al. 2014. "Identification of a Bioactive Compound against Adult T-cell Leukaemia from Bitter Gourd Seeds" Plants 3, no. 1: 18-26. https://doi.org/10.3390/plants3010018