Assessment of Toxicological Perturbations and Variants of Pancreatic Islet Development in the Zebrafish Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fish
2.2. Chemicals
2.3. Exposures
2.4. Microscopy
2.5. Statistics
3. Results
3.1. Quantification of Islet Area among Treatment Control Embryos
3.2. Identification of Islet Anomalies under Control Conditions
3.3. Contaminant-Induced Abnormalities of the Primary Pancreatic Islet
3.3.1. Cyclopamine
3.3.2. MEHP
3.3.3. PFOS
3.3.4. tBOOH
4. Discussion
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Variant Phenotype | Control (DMSO 1) | MEHP 2 (0.7 µM) |
---|---|---|
Fragmented Islets | 5/29 (17%) | 4/27 (15%) |
Ectopic Beta Cells | 0/29 (0%) | 3/27 (11%) |
Total Variants | 5/29 (17%) | 7/27 (26%) |
Variant Phenotype | Control (DMSO) | PFOS 1 (32 µM) |
---|---|---|
Fragmented Islets | 1/37 (3%) | 3/39 (18%) |
Ectopic Beta Cells | 1/37 (3%) | 1/39 (3%) |
Stunted Islets | 1/37 (3%) | 11/39 (28%) |
Total Variants | 3/37 (8%) | 15/39 (38%) |
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Sant, K.E.; Jacobs, H.M.; Xu, J.; Borofski, K.A.; Moss, L.G.; Moss, J.B.; Timme-Laragy, A.R. Assessment of Toxicological Perturbations and Variants of Pancreatic Islet Development in the Zebrafish Model. Toxics 2016, 4, 20. https://doi.org/10.3390/toxics4030020
Sant KE, Jacobs HM, Xu J, Borofski KA, Moss LG, Moss JB, Timme-Laragy AR. Assessment of Toxicological Perturbations and Variants of Pancreatic Islet Development in the Zebrafish Model. Toxics. 2016; 4(3):20. https://doi.org/10.3390/toxics4030020
Chicago/Turabian StyleSant, Karilyn E., Haydee M. Jacobs, Jiali Xu, Katrina A. Borofski, Larry G. Moss, Jennifer B. Moss, and Alicia R. Timme-Laragy. 2016. "Assessment of Toxicological Perturbations and Variants of Pancreatic Islet Development in the Zebrafish Model" Toxics 4, no. 3: 20. https://doi.org/10.3390/toxics4030020
APA StyleSant, K. E., Jacobs, H. M., Xu, J., Borofski, K. A., Moss, L. G., Moss, J. B., & Timme-Laragy, A. R. (2016). Assessment of Toxicological Perturbations and Variants of Pancreatic Islet Development in the Zebrafish Model. Toxics, 4(3), 20. https://doi.org/10.3390/toxics4030020