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Article

The Phylogeny and Taxonomy of Cryptothecia (Arthoniaceae, Ascomycota) and Myriostigma (Arthoniaceae, Ascomycota), including Three New Species and Two New Records from China

by
Junxia Xue
,
Yutong Cai
and
Lulu Zhang
*
Institute of Environment and Ecology, Shandong Normal University, Jinan 250300, China
*
Author to whom correspondence should be addressed.
J. Fungi 2024, 10(4), 274; https://doi.org/10.3390/jof10040274
Submission received: 6 March 2024 / Revised: 30 March 2024 / Accepted: 8 April 2024 / Published: 9 April 2024
(This article belongs to the Special Issue Ascomycota: Diversity, Taxonomy and Phylogeny, 2nd Edition)
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Abstract

:
Cryptothecia and Myriostigma are important elements of crustose lichen communities in tropical to subtropical forests, but little research has been done on these two genera in China. Morphological and molecular phylogenetic approaches to investigate species diversity of Cryptothecia and Myriostigma from Southern China were carried out in this study. We find five species of Cryptothecia and Myriostigma in our study, including three new species (M. flavescens, M. hainana and M. laxipunctata) and two new records (C. bartlettii and C. inexspectata). In addition, a phylogenetic tree based on mtSSU, RPB2 and nLSU illustrates the placement of the five species and supports the delimitation of the three new taxa. Detailed descriptions of morphological, ecological and chemical characteristics and illustrations are provided for every species. A key to all known Chinese Cryptothecia and Myriostigma species is also provided.

1. Introduction

Cryptothecia Stir. is a genus of lichenized fungi belonging to Arthoniaceae, Arthoniales, Arthoniomycetes, Ascomycota [1]. The genus Cryptothecia is recognized by corticolous, ecorticate, greenish grey to whitish grey thalli; whitish byssoid prothalli; a medulla with calcium oxalate crystals; trentepohlioid photobiont, yellowish green; broadly clavate to globose, thick-walled asci loosely dispersed on the thallus or closely aggregated within ascigerous areas; and muriform ascospores [2]. Almost all Cryptothecia species are fertile, except C. isidioxantha Aptroot & M. Cáceres, C. duplofluorescens Aptroot & Souza, C. reagens Aptroot & Schumm, and C. lecanorosorediata Aptroot & M. Cáceres which are not known to produce ascospores [3,4,5,6].
Recently, phylogenetic analyses revealed that Cryptothecia was polyphyletic and Myriostigma Krempelhuber (1874) was resurrected because the type species Myriostigma candidum Kremp. [=Cryptothcia candida (Kremp.) R. Sant.] represents a lineage separate from Cryptothecia. Myriostigma species are characterized by raised maculate ascomata-like ascigerous areas; eight-spored, thick-walled, globular and stalked asci; and bean-shaped, muriform ascospores [7].
Cryptothecia and Myriostigma are distributed in tropical and subtropical humid forests. To date, approximately 113 species are known worldwide [6,8,9,10,11,12,13,14,15,16], and only 6 species have been reported in China [17,18]. During our research on these two genera, three new species (M. flavescens J.X. Xue & Lu L. Zhang, M. hainana J.X. Xue & Lu L. Zhang and M. laxipunctata J.X. Xue & Lu L. Zhang) and two new records (C. bartlettii G. Thor and C. inexspectata G. Thor) were found.

2. Materials and Methods

2.1. Morphology and Anatomy

The specimens examined were collected in Fujian, Guangxi, Hainan and Yunnan Provinces, China, and preserved in the Lichen Section of Botanical Herbarium (SDNU, Shandong Normal University). External morphological features were studied with a dissecting microscope (COIC XTL7045B2) (Olympus, Toyko, Japan), and photos were taken under an Olympus SZX16 (Olympus, Toyko, Japan) with DP72. Internal anatomical features were observed and measured by a microscope (Olympus CX41) (Olympus, Toyko, Japan), and images were taken with an Olympus BX61 (Olympus, Toyko, Japan) with DP 72.

2.2. Colour Reaction

The thallus and medulla colouring reactions were carried out by P (a saturated solution of p-phenylenediamine in 95% ethyl alcohol) (Tianjin Fuyu Fine Chemical Co., Tianjin, China), C (a saturated solution of aqueous sodium hypochlorite) (Tianjin Fuyu Fine Chemical Co., Tianjin, China), K (a 10% aqueous solution of potassium hydroxide) (Tianjin Damao Chemical FTY., Tianjin, China), I (a 3% solution of Lugol’s iodine) (Tianjin Fuyu Fine Chemical Co., Tianjin, China), and long-wavelength UV light. Polarized light microscopy (pol) was used to locate crystals in the thallus sections. Then, H2SO4 (a 25% solution of sulfuric acid) (Yantai Yuandong Fine Chemical Co., Yantai, China), was used to test whether the characteristic needle-shaped crystals formed when calcium oxalate was present in the thallus.

2.3. Chemical Analysis

The lichen substances were identified using standardized thin-layer chromatography (TLC). The particularly stable and reliable solvent C (toluene/acetic acid 170:30) was used in this study as it often provides the best discrimination of lichen substances. In this study, Lethariella cladonioides (Nyl.) Krog. containing atranorin and norstictic acid was used as the partition standard sample.
We used a pencil, carefully drawing a straight line 1.5 cm from the bottom of the glass silicone board. A point was marked every 1 cm on the straight line, which was the sample point. We soaked the lichen fragments in c. 1 mL of acetone for 30 min in a small test tube. Then, we used microcapillary tubes to sample them separately according to the position of the sampling points on the glass silicone board. After sampling, we placed the silicone board in a chromatography cylinder and placed it 1 cm below the solvent level. When the leading edge of the solvent moved from the origin to about 1 cm from the top of the silicone board, we removed the silicone board and dried the solvent on the board surface with a hair dryer. The silicone board was dried and then examined under short wavelength (254 nm) ultraviolet light for pigments. Later, it was sprayed with 10% sulfuric acid and heated at 100 °C in an oven for 10 min to develop the spots. The Rf values and color of each lichen substance were recorded and immediately examined under long wavelength (365 nm) ultraviolet light. The Rf values, as well as the fluorescent properties, were compared and analyzed to confirm the identity of the substance [19,20].

2.4. DNA Extraction, PCR Amplification and Sequencing

DNA was extracted directly from the clean growing portions of the thalli of recently collected specimens. Genomic DNA was extracted using the Sigma-Aldrich REDExtract-N-Amp Plant PCR Kit (St. Louis, MO, USA) following the manufacturer’s instructions, except for the use of only 30 μL of extraction buffer and 30 μL of dilution buffer.
In our study, we found that the ITS sequence, typically considered crucial for distinguishing between lichen species, is essentially identical in Cryptothecia and Myriostigma and cannot be used as a standard for distinguishing between species within these two genera. Therefore, three other loci were amplified: the mtSSU gene with primer pairs mtSSU1 and mtSSU3R [21], the RPB2 gene with RPB2-7cF and RPB2-11aR [22], and the nLSU gene with LIC24R and LR7 [23,24]. The 50 μL PCR mixture consisted of 2 μL of DNA, 2 μL of each primer, 25 μL of 2 × Taq PCR MasterMix [Taq DNA Polymerase (0.1 unit/μL); 3 mM MgCl2; 100 mM KCl; 0.5 mM dNTPs; and 20 mM Tris-HCl (pH 8.3)] (Tiangen, Beijing, China), and 19 μL of dd H2O. The PCR conditions for mtSSU amplification were set for initial denaturation at 94 °C for 10 min; 34 cycles of 95 °C for 45 s, 50 °C for 45 s, and 72 °C for 90 s; and a final extension at 72 °C for 10 min. The PCR conditions for RPB2 amplification were set for an initial denaturation at 94 °C for 10 min; 34 cycles of 94 °C for 45 s, 52 °C for 50 s, and 72 °C for 1 min; and a final extension at 72 °C for 5 min. The PCR conditions for nLSU amplification were set for an initial denaturation at 95 °C for 15 min; 45 cycles of 95 °C for 45 s, 53 °C for 45 s, and 72 °C for 1 min; and a final extension at 72 °C for 7 min. Sequencing was performed by BioSune Biological Technology (Shanghai, China).

2.5. Sequence Alignment and Phylogenetic Analysis

To ascertain that all the new sequences were reliable, the newly generated sequences were compared to the sequences available in the GenBank database (http://www.ncbi.nlm.nih.gov/BLAST/, accessed on 10 September 2023), and all the new sequences were assembled using SeqMan v.7.0 (DNAstar packages). The sequences were aligned and edited using the online version of MAFFT v.7.0.26 and MEGA v.7.0. The algorithm of MAFFT chooses automatically (FFT-NS-1, FFT-NS-2, FFT-NS-i or L-INS-i; depending on the data size). The species Chiodecton natalense Nyl. was chosen as the outgroup [12].
Phylogenetic relationships were inferred using maximum likelihood (ML) and Bayesian inference (BI). The three gene matrices were combined by Geneious Prime 2023.0.4. We used the CIPRES Science Gateway (http://www.phylo.org/portal2/, accessed on 1 March 2024) [25] and performed ML analyses using RaxML-HPC v. 8.2.12 [26] under the default parameters as implemented on CIPRES, and support values were based on 1000 nonparametric bootstrap pseudoreplicates. We used PhyloSuite [27] to infer BI phylogenies via MrBayes 3.2.6 [28] under a partition model, for which the initial 25% of the sampled data were discarded as burn-in. The stationarity of the analysis was determined by examining the standard deviation of the split frequencies (<0.01). Bootstrap support (BS) ≥ 70 and posterior probability (PP) ≥ 0.95 were considered significant support values. The phylogenetic trees generated were visualized with FigTree v. 1.4.2 [29].

3. Results

3.1. Phylogenetic Analyses

A total of 10 mtSSU sequences, 7 RPB2 sequences and 2 nLSU sequences were newly generated from 10 specimens. We constructed ML and BI topologies based on these mtSSU, RPB2 and nLSU sequences and 87 additional sequences downloaded from NCBI (https://www.ncbi.nlm.nih.gov/, accessed on 25 February 2024) (Table 1). The phylogenetic trees obtained from ML and BI analyses exhibited similar topologies; therefore, we present only the ML tree, with BS ≥ 70 for the ML analysis and PP ≥ 0.95 for the Bayesian analysis (Figure 1).
The combined sequence matrices revealed three new monophyletic lineages corresponding to three new species: Myriostigma flavescens J.X. Xue & Lu L. Zhang, sp. nov.; M. hainana J.X. Xue & Lu L. Zhang, sp. nov.; and M. laxipunctata J.X. Xue & Lu L. Zhang, sp. nov. All the species positions had strong support in the ML and Bayesian analyses. In our phylogenetic tree, we can see those three new species (M. flavescens, M. hainana and M. laxipunctata) are clustered with Myriostigma and Stirtonia. According to Aptroot [30], Stirtonia had transversely septate ascospores; thus, based on morphological characters and phylogenetic analysis, we propose three new species in Myriostigma. This is in addition to two new Chinese records, as both are clustered with Cryptothecia subnidulans (the type species of Cryptothecia), which supported the delimitation of two new Chinese records.

3.2. Taxonomy

3.2.1. The New Species

Myriostigma flavescens J.X. Xue & Lu L. Zhang, sp. nov. (Figure 2)
MycoBank No: 850048
Diagnosis: The new species differs from other species of Myriostigma by ascigerous areas with brown dots and erumpent angular to irregular outlines, yellow ascospores (58–76 × 19–28 µm), gyrophoric acid and confluentic acid as secondary metabolites.
Type: CHINA, Yunnan Province, Xishuangbanna Dai Nationality Autonomous Prefecture, Jinghong City, Primitive Forest Park, 22°1′55.75″ N, 100°52′37.47″ E, alt. 689 m, on bamboo, 7 March 2023, L.L. Liu et al. 20230612 (SDNU, holotype).
GenBank accession numbers: SSU PP051268, PP051267; RPB2 PP130144.
Description: Thallus corticolous or bambusicolous, up to 3 cm in diameter, ecorticate, cottony, dull, greenish grey, loosely attached to the substrate, 50–70 µm thick. Isidia not observed. Prothallus usually distinct, linear shaped, dark brown to black. Medulla white, with calcium oxalate crystals. Photobiont trentepohlioid, cells rounded to elliptical, single or a few cells aggregated, 6–17 × 6–13 µm. Hyphae 1.5–2 µm wide.
Ascigerous areas distinct, generally delimited, erumpent, slightly raised above the thallus level, angular to irregular in outline, 1–5 mm diam. and 115–165 µm tick, white with dense brown dots indicate individual asci. Asci pale yellow, frequent, fragile, globose to subglobose, often covered by hyaline hyphae, 8-spored, 95–100 × 83–95 µm. Ascospores yellow, oblong, muriform, curved, often broader in the centre, 58–76 × 19–28 µm.
Pycnidia not observed.
Chemistry: thallus C+ red, K–, P–, UV+ pale grey-white; medulla and paraphysoids I+ sky-blue. TLC: gyrophoric acid and confluentic acid.
Etymology: The epithet refers to its yellow ascospores.
Ecology and distribution: This species is found only in China on bamboo and trees in a humid tropical forest in Yunnan Province.
Notes: Morphologically, Myriostigma flavescens is similar to M. candidum Kremp. in having irregular erumpent whitish ascigerous areas, but M. candidum has smaller ascospores (40–65 × 12–25 µm) and 2′-O-methylanziaic acid and 2′-O-methylperlatolic acid as secondary metabolites [31]. In addition, Myriostigma flavescens is similar to M. filicinum (Ellis & Everh.) Frisch & G. Thor, but the latter has rounded to somewhat irregular outlines of ascigerous areas, smaller asci (60–90 × 50–70 µm) and perlatolic acid as a secondary metabolite [31].
Additional specimens examined: CHINA, Yunnan Province, Xishuangbanna Dai Nationality Autonomous Prefecture, Jinghong City, Primitive Forest Park, 22°1′55.75″ N, 100°52′37.47″ E, alt. 689 m, on bamboo, 7 March 2023, L.L. Liu et al. 20230641 (SDNU); ibid., 20230623 (SDNU); ibid., 22°2′9.71″ N, 100°53′5.81″ E, alt. 746 m, on the bark of trees, 7 March 2023, L.L. Liu et al. 20230695 (SDNU).
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Figure 2. Myriostigma flavescens (SDNU 20230612, holotype). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A), 200 µm (B), 10 µm (C,D).
Figure 2. Myriostigma flavescens (SDNU 20230612, holotype). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A), 200 µm (B), 10 µm (C,D).
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Myriostigma hainana J.X. Xue & Lu L. Zhang, sp. nov. (Figure 3)
MycoBank No: 849419
Diagnosis: The new species differs from other species of Myriostigma by having I– medulla, gyrophoric acid and methyl 2′-O-methylmicrophyllinate as secondary metabolites.
Type: CHINA, Hainan Province, Wenchang City, Beigang Village, 19°32′46.30″ N, 110°51′10.44″ E, alt. 26 m, on the bark of trees, 7 March 2023, J.X. Xue & Y.M. Zhang 20230061 (SDNU, holotype).
GenBank accession numbers: SSU PP051271, PP051272; RPB2 PP101845, PP109365.
Description: Thallus corticolous, up to 4 cm in diameter, ecorticate, cottony, dull, greenish grey to whitish grey, loosely attached to the substrate, 100–162 µm thick. Isidia not observed. Prothallus usually distinct, thin, whitish byssoid, mainly composed of interwoven hyphae, 0.5–1 mm wide, forming a dark brown line while bordering different species. Medulla white, with calcium oxalate crystals. Photobiont trentepohlioid, cells elliptical to oblong, single or a few cells aggregated, 9–15 × 6–9 µm. Hyphae 1–2 µm wide.
Ascigerous areas indistinct, not delimited. Asci frequent, fragile, globose to subglobose, scattered and immersed across the thallus, often covered by hyaline hyphae, 8-spored, 120–138 × 120–135 µm; sometimes 1–6(–7) spores aborted, thus, these asci remain (1–)2–7-spored. Ascospores hyaline, pale yellow when mature, oblong, muriform, straight, often broader in the centre, 52–88 × 24–47 µm.
Pycnidia not observed.
Chemistry: thallus C+ red, K–, P–, UV–; medulla and paraphysoids I–. TLC: gyrophoric acid and methyl 2′-O-methylmicrophyllinate.
Etymology: The epithet refers to the discovery of this new species in Hainan Province, China.
Ecology and distribution: This species is found only in China on the bark of trees in a humid tropical forest in Hainan Province.
Notes: Morphologically, Myriostigma hainana is the first species of the genus Myriostigma that contains 2′-O-methylmicrophyllinate. It is similar to Cryptothecia subtecta Stirt. in having indistinct ascigerous areas, but C. subtecta has an I+ blue medulla, smaller ascospores (27–40 × 12–20 µm) and confluentic acid as secondary metabolites [32]. In addition, Myriostigma hainana is similar to Cryptothecia odishensis R. Bajpai, S. Joseph & Upreti, but the latter has an I+ blue medulla, larger ascospores (105–142 × 35–52 µm) and barbatic acid and gyrophoric acid as secondary metabolites [33].
Phylogenetically, Myriostigma hainana is clustered with Myriostigma miniatum (Vain. ex Lücking) Aptroot, Ertz, Grube & M. Cáceres. They both have 8-spored asci, but M. miniatum has dark grey prothallus, bright orange ascigerous areas and 2-O-demethylperlatolic acid and orange pigment as secondary metabolites [34].
Additional specimens examined: CHINA, Hainan Province, Wenchang City, Dongjiao Town, Beigang Village, 19°32′46.30″ N, 110°51′10.44″ E, alt. 26 m, on the bark of trees, 7 March 2023, J.X. Xue & Y.M. Zhang 20230050 (SDNU); ibid., 20230051 (SDNU); ibid., Prima Resort, 19°31′51.02″ N, 110°50′54.14″ E, alt. 26 m, on the bark of trees, 6 March 2023, J.X. Xue & Y.M. Zhang 20230242 (SDNU).
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Figure 3. Myriostigma hainana (SDNU 20230061, holotype for (A,C,D); SDNU 20230051, type for (B)). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A), 200 µm (B), 10 µm (C,D).
Figure 3. Myriostigma hainana (SDNU 20230061, holotype for (A,C,D); SDNU 20230051, type for (B)). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A), 200 µm (B), 10 µm (C,D).
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Myriostigma laxipunctata J.X. Xue & Lu L. Zhang, sp. nov. (Figure 4)
MycoBank No: 850046
Diagnosis: The new species differs from other species of Myriostigma with its ascigerous areas having loose brown dots, slightly raised above the level of the thallus; hyaline ascospores (57–78 × 24–33 µm); gyrophoric acid and confluentic acid as secondary metabolites.
Type: CHINA, Yunnan Province, Jinghong City, Mengla County, Menglun Town, Xishuangbanna Tropical Botanical Garden, 21°55′43.17″ N, 101°15′39.08″ E, alt. 525 m, on the bark of trees, 5 March 2023, L.L. Liu et al. 20231052 (SDNU, holotype).
GenBank accession numbers: SSU PP051266, PP051265; RPB2 PP109369, PP109368; LSU PP033944, PP033943.
Description: Thallus corticolous, up to 4.5 cm in diameter, ecorticate, cottony, dull, pale green, firmly attached to the substrate, 75–90 µm thick. Isidia not observed. Prothallus usually distinct, thin, whitish byssoid, mainly composed of interwoven hyphae, 1–2 mm wide, forming a dark brown line while bordering different species. Medulla white, with calcium oxalate crystals. Photobiont trentepohlioid, cells rounded to oblong, single or a few cells aggregated, 7–10 × 5–8 µm. Hyphae 1–2.5 µm wide.
Ascigerous areas generally delimited, erumpent, developing in the thallus centre, slightly raised above the thallus level, pale greenish and with loose brown dots indicating individual asci, 140–175 µm thick. Asci hyaline, fragile, globose to subglobose, often covered by hyaline hyphae, 8-spored, 95–124 × 93–119 µm. Ascospores hyaline, oblong, muriform, straight or curved, often broader in the centre, 57–78 × 24–33 µm.
Pycnidia not observed.
Chemistry: thallus C+ red, K–, P–, UV+ pale grey-white; medulla and paraphysoids I+ sky-blue. TLC: gyrophoric acid and confluentic acid.
Etymology: The epithet refers to the ascigerous areas of the thallus that have loose brown dots.
Ecology and distribution: This species is found only in China on the bark of trees in a humid tropical forest in Yunnan Province.
Notes: Morphologically, Myriostigma laxipunctata is similar to Myriostigma irregulare (Lücking, Aptroot, Kalb & Elix) Frisch & G. Thor, but the latter has radiately elongated ascigerous areas, short-stalked asci and psoromic acid, subpsoromic acid, 2′-O-demethylpsoromic acid and confluentic acid as secondary metabolites [31]. In addition, Myriostigma laxipunctata is similar to Cryptothecia stockeri G. Neuwirth & Aptroot in having a firmly attached thallus and brown punctate ascigerous areas, but C. stockeri has a brown linear shaped prothallus, smaller ascospores (25–40 × 12–15 µm) and psoromic acid as a secondary metabolite [35].
Phylogenetically, Myriostigma laxipunctata is clustered with Myriostigma flavescens J.X. Xue & Lu L. Zhang and M. candidum Kremp. They both have 8-spored asci, but M. flavescens has white erumpent ascigerous areas with dense brown dots and yellow ascospores, M. candidum has smaller ascospores (40–65 × 12–25 µm) and 2′-O-methylperlatolic acid and 2′-O-methylanziaic acid as secondary metabolites [31].
Additional specimens examined: CHINA, Yunnan Province, Jinghong City, Mengla County, Menglun Town, Xishuangbanna Tropical Botanical Garden, 21°53′25.66″ N, 101°16′9.91″ E, alt. 592 m, on the bark of trees, 5 March 2023, L.L. Liu et al. 20231231 (SDNU); ibid., 21°55′30.14″ N, 101°15′42.72″ E, alt. 527 m, on the bark of trees, 5 March 2023, L.L. Liu et al. 20231124 (SDNU).
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Figure 4. Myriostigma laxipunctata (SDNU 20231052, holotype). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A,B), 10 µm (C,D).
Figure 4. Myriostigma laxipunctata (SDNU 20231052, holotype). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A,B), 10 µm (C,D).
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3.2.2. The New Records

Cryptothecia bartlettii G. Thor, Symb. Bot. Ups. 32:1, 277 (1997) (Figure 5)
GenBank accession numbers: SSU PP051262, PP051261.
Description: Thallus corticolous, up to 7 cm in diameter, ecorticate, thick, cottony, dull, greenish grey to greyish white, loosely attached to the substrate, with paler, sometimes slightly raised areas, 175–260 µm thick. Isidia not observed, but the thallus had globose isidia-like structures (it does not contain cortex, but isidia contains cortex). Prothallus usually distinct, thick, whitish byssoid, mainly composed of interwoven hyphae, 0.5–1.5 mm wide. Medulla white, with calcium oxalate crystals. Photobiont trentepohlioid, cells rounded to oblong, single or aggregate into bundles, 9–15 × 7–12 µm. Hyphae 1–2 µm wide.
Ascigerous areas generally delimited, developing in the thallus centre, generally covered with globose isidia-like structures. Asci frequent, hyaline, ellipsoid to oblong, with a stalk, weakly aggregated, often covered by hyaline hyphae, 1-spored, 100–138 × 48–64 µm. Ascospores hyaline, ovoid to oblong, muriform, often slightly constricted just below the middle, surrounded by abundant cytoplasm in the asci when mature, (49–)68–100(–105) × (18–)23–36(–42) µm.
Pycnidia not observed.
Chemistry: thallus C+ red, K–, P–, UV+ pale grey-white; medulla and paraphysoids I+ sky-blue. TLC: gyrophoric acid.
Ecology and distribution: Cryptothecia bartlettii has previously been reported in New Zealand and Australia [2] and is new to China. We collected the species in Fujian Province under similar ecological conditions as in New Zealand and Australia. Specifically, we found them on the bark of trees within the subtropical forest at an elevation of approximately 450 to 570 m.
Notes: According to Thor [2], Cryptothecia bartlettii is characterized by a thick and loosely attached thallus, ascigerous areas covered by globose isidia-like structures and large ascospores [(49–)68–100(–105) × (18–)23–36(–42) µm]. The Chinese collections agree with this description, except for the longer asci (100–138 × 48–64 µm vs. 90–105 × 50–64 µm) and larger ascospores [(49–)68–100(–105) × (18–)23–36(–42) µm vs. (53–)69–84(–96) × (21–)25–30(–33) µm]. Cryptothecia bartlettii is similar to C. eungellae G. Thor in having a grey greenish thallus and globose isidia-like structures, but C. eungellae has a firmly attached thallus, shorter ascospores (55–72 µm long) and gyrophoric acid and norstictic acid as secondary metabolites [11].
Phylogenetically, Cryptothecia bartlettii is clustered with C. inexspectata G. Thor and C. subnidulans Stirt.; they all have 1-spored asci, but C. inexspectata has radiate and elongated whitish ascigerous areas and smaller ascospores (33–50 × 16–22 µm), and C. subnidulans has a firmly attached thallus, psoromic acid as a secondary metabolite and the ascigerous area without globose isidia-like structures [2].
Specimens examined: CHINA, Fujian Province, Fuzhou City, Jinan District, Gushan Town, Mt. Gushan, 26°03′22.99″ N, 119°23′16.10″ E, alt. 458 m, on the bark of trees, 13 July 2022, Lu L. Zhang & Q.J. Zuo 20220280 (SDNU); ibid., 26°03′43.78″ N, 119°23′14.49″ E, alt. 572 m, on the bark of trees, 13 July 2022, Lu L. Zhang & Q.J. Zuo 20220297 (SDNU); ibid., 26°03′19.51″ N, 119°23′10.64″ E, alt. 446 m, on the bark of trees, 13 July 2022, Lu L. Zhang & Q.J. Zuo 20220275 (SDNU).
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Figure 5. Cryptothecia bartlettii (SDNU 20220280). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 1 mm (A), 500 µm (B), 10 µm (C,D).
Figure 5. Cryptothecia bartlettii (SDNU 20220280). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 1 mm (A), 500 µm (B), 10 µm (C,D).
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Cryptothecia inexspectata G. Thor, Symb. Bot. Ups. 32:1, 283 (1997) (Figure 6)
GenBank accession numbers: SSU PP051263, PP051264; RPB2 PP109371, PP109371.
Description: Thallus corticolous or bambusicolous, up to 4.5 cm in diameter, ecorticate, cottony, dull, greenish grey to whitish grey, sometimes yellowish green, firmly attached to the substrate, 85–100 µm thick. Isidia not observed. Prothallus usually distinct, thin, whitish byssoid, mainly composed of interwoven hyphae, 1–2 mm wide. Medulla white, with calcium oxalate crystals. Photobiont trentepohlioid, single or a few cells aggregated, cells rounded to oblong, 8–17 × 7–14 µm. Hyphae 1–3 µm wide.
Ascigerous areas generally delimited, whitish and slightly raised above the thallus level, irregular in outline and usually radiately elongated, aggregated in the thallus centre. Asci hyaline, oblong to obovate, with a stalk, weakly aggregated, often covered by hyaline hyphae, 1-spored, 41–60 × 31–40 µm. Ascospores hyaline, ovoid, muriform, often slightly constricted just below the middle, surrounded by abundant cytoplasm in the asci when mature, 33–50 × 16–22 µm.
Pycnidia not observed.
Chemistry: thallus C+ red, K–, P–, UV+ pale grey-white; medulla and paraphysoids I+ sky-blue. TLC: gyrophoric acid.
Ecology and distribution: Cryptothecia inexspectata has previously been reported in Australia, Papua New Guinea and Java [2,8] and is new to China. We collected the species in Fujian and Yunnan Provinces under similar ecological conditions as in Australia, Papua New Guinea and Java. Specifically, we found them on the bark of trees and on bamboo within the subtropical forest and tropical bamboo forest at an elevation of approximately 360 to 690 m.
Notes: According to Thor [2], Aptroot and Spier [8], Cryptothecia inexspectata is characterized by a firmly attached thallus and radiately elongated whitish ascigerous areas. The Chinese collections agree with this description, except for the shorter asci (41–60 µm long vs. 60–80 µm long). Cryptothecia inexspectata is similar to C. striata G. Thor in having radiately elongated whitish ascigerous areas, but C. striata has granular isidia and larger ascospores [(46–)55–70(–80) × (19–)23–29(–37) µm] [36].
Specimens examined: CHINA, Guangxi Province, Liuzhou City, Rongshui County, Qingshui Pond Protection Station, 25°11′59.79″ N, 108°47′46.47″ E, alt. 359 m, on the bark of trees, 19 December 2020, L. Hu et al. 20200434 (SDNU); ibid., 20200378 (SDNU); Yunnan Province, Xishuangbanna Dai Autonomous Prefecture, Jinghong City, Primitive Forest Park, 22°1′55.75″ N, 100°52′37.47″ E, alt. 689 m, on bamboo, 7 March 2023, L.L. Liu et al. 20230639 (SDNU); ibid., 22°2′9.71″ N, 100°53′5.81″ E, alt. 716 m, on the bark of trees, 7 March 2023, L.L. Liu et al. 20230668 (SDNU).
Jof 10 00274 g006
Figure 6. Cryptothecia inexspectata (SDNU 20200434 for (A,B); SDNU 20230639 for (C,D)). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A), 200 µm (B), 10 µm (C,D).
Figure 6. Cryptothecia inexspectata (SDNU 20200434 for (A,B); SDNU 20230639 for (C,D)). (A) Thallus and prothallus. (B) Ascigerous areas. (C) Asci. (D) Ascospores. Scale bars: 500 µm (A), 200 µm (B), 10 µm (C,D).
Jof 10 00274 g006
Key to the Cryptothecia and Myriostigma species occurring in China
1a.Asci 1–2-spored—2
1b.Asci 8-spored—4
2a.Thallus P+ yellow; with psoromic acid—C. subnidulans
2b.Thallus P–; without psoromic acid—3
3a.Ascigerous areas whitish and usually radiately elongated; ascospores
33–50 × 16–22 µm—C. inexspectata
3b.Ascigerous areas generally covered with globose isidia-like structures; ascospores (49–)68–100(–105) × (18–)23–36(–42) µm—C. bartlettii
4a.Thallus without lichen substances—5
4b.Thallus with lichen substances—6
5a.Ascospores narrow; 60–76 × 17–30 µm—C. aleurella
5b.Ascospores broad; 65–108 × 42–50 µm—C. aleurocarpa
6a.Thallus C+ red; with gyrophoric or 2′-O-methylanziaic acids—7
6b.Thallus C–; without gyrophoric or 2′-O-methylanziaic acids—10
7a.Thallus with gyrophoric acid and additional substances—8
7b.Thallus with 2′-O-methylanziaic and 2′-O-methylperlatolic acids; ascospores
40–65 × 12–25 µm—M. candidum
8a.Ascigerous areas indistinct; additionally with methyl 2′-O-methylmicrophyllinate; ascospores 52–88 × 24–47 µm—M. hainana
8b.Ascigerous areas distinct; additionally with confluentic acid—9
9a.Ascigerous areas white with dense brown dots; ascospores yellow;
58–76 × 19–28 µm—M. flavescens
9b.Ascigerous areas pale greenish with loose brown dots; ascospores hyaline;
57–78 × 24–33 µm—M. laxipunctata
10a.Thallus P+ yellow; with psoromic acid; ascospores 50–70 × 30–37 µm—C. polymorpha
10b.Thallus P–; without psoromic acid; ascospores 27–40 × 12–20 µm—C. subtecta

4. Discussion

As one of the largest families of lichens, Arthoniaceae contains more than 700 lichenized species according to Lücking et al. [37]. Recent phylogenetic analyses by Frisch et al. [7], Thiyagaraja et al. [38] and others have further implications for our understanding of generic limits within this family. However, it should be noted that all of these analyses were based on only a relatively low number of species described in Arthoniaceae. In previous studies, we can see that the morphological characteristics of Cryptothecia and Myriostigma are very similar, and some species of Myriostigma were described as Cryptothecia. In 2014, Frisch et al. [7] revealed through phylogenetic analysis that Cryptothecia was polyphyletic and Myriostigma Krempelhuber (1874) was resurrected because the type species Myriostigma candidum Kremp. [=Cryptothcia candida (Kremp.) R. Sant.] represents a lineage separate from Cryptothecia. They also reinstated Myriostigma for the Cryptothecia candida group, which is characterized by raised maculate ascomata-like ascigerous areas; 8-spored, thick-walled, globular and stalked asci; and bean-shaped, muriform ascospores [7]. Therefore, some species of Cryptothecia, such as C. miniata and C. napoensis, were transferred to Myriostigma [10,34]. However, in our study, we can see that some species of Myriostigma, such as M. hainana and M. laxipunctata, resemble Cryptothecia in morphology due to the absence of raised maculate ascomata-like ascigerous areas; thus, the distinction between the two genera based on the presence or absence of a maculate ascomata-like ascigerous areas becomes ambiguous. In addition, in our research, all species of Cryptothecia formed a cluster with the type species, C. subnidulans Stirt., on the phylogenetic trees, which exhibited a consistent presence of 1–2-spored asci, and all species of Myriostigma have 8-spored asci. The number of ascospores may thus serve as a distinguishing characteristic between Cryptothecia s. str. and Myriostigma. The confirmation of this viewpoint requires further research.
Species in Arthoniaceae often have a pantropical distribution. In southern China, there are abundant subtropical to tropical evergreen resources [39]. This habitat is favourable for the lichens of Arthoniaceae. However, the family has not been sufficiently studied; thus far, only a few species have been recorded in China, including five species of Cryptothecia and one species of Myriostigma [40]. In our study, we found that Arthoniaceae is highly diverse in southern China, especially in the Xishuangbanna region of Yunnan Province. Therefore, more comprehensive sampling and research are needed to determine the true diversity of Arthoniaceae in southern China.

Author Contributions

Conceptualization, L.Z.; methodology, J.X.; software, J.X.; validation, J.X.; formal analysis, J.X. and L.Z.; investigation, J.X. and Y.C.; data curation, J.X.; writing—original draft preparation, J.X.; writing—review and editing, J.X., Y.C. and L.Z.; visualization, J.X. and Y.C.; supervision, L.Z.; project administration, L.Z.; funding acquisition, L.Z. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the National Natural Science Foundation of China (No. 32170002, 31750001).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The names of the new species were formally registered in the MycoBank database (https://www.mycobank.org/, accessed on 16 September 2023). Specimens were deposited in the Lichen Section of the Botanical Herbarium, Shandong Normal University (SDNU). The newly generated sequences were deposited in GenBank (https://www.ncbi.nlm.nih.gov/genbank, accessed on 24 December 2023).

Acknowledgments

The authors would like to thank L.L. Liu, Q.J. Zuo, L. Wang, C.J. Zhong and Y.M. Zhang for collecting specimens jointly for this study. The authors also would like to thank André Aptroot (UFMS University, Campo Grande, Brazil) for his valuable suggestions.

Conflicts of Interest

The authors declare no conflicts of interest.

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Figure 1. Phylogenetic tree constructed by maximum likelihood (ML) analysis of Arthoniaceae species based on the concatenated mtSSU-RPB2-nLSU dataset. Bootstrap support values ≥ 70 for ML and posterior probabilities ≥ 0.95 (second value) for Bayesian methods are indicated above or below the branches. Newly obtained sequences are marked in bold.
Figure 1. Phylogenetic tree constructed by maximum likelihood (ML) analysis of Arthoniaceae species based on the concatenated mtSSU-RPB2-nLSU dataset. Bootstrap support values ≥ 70 for ML and posterior probabilities ≥ 0.95 (second value) for Bayesian methods are indicated above or below the branches. Newly obtained sequences are marked in bold.
Jof 10 00274 g001
Table 1. Specimens used for the phylogenetic analyses with the corresponding voucher information and GenBank accession numbers for the mtSSU, RPB2 and nLSU sequences. Newly obtained sequences in this study are in bold.
Table 1. Specimens used for the phylogenetic analyses with the corresponding voucher information and GenBank accession numbers for the mtSSU, RPB2 and nLSU sequences. Newly obtained sequences in this study are in bold.
Species NameVoucher SpecimenGenBank Accession Number
mtSSURPB2nLSU
Arthonia calcareaThor 11/6a (UPS)KJ850974KJ851105–
Arthonia didymaErtz 7587 (BR)EU704047EU704010EU704083
Arthonia granitophilaFrish 10/Se74 (UPS)KJ850981KJ851107KJ851049
Arthonia graphidicolaFrisch 10/Jp102 (UPS)KJ850980–KJ851034
Arthonia ilicinaMcCune 31067KJ850982––
Arthonia radiataFrisch 10/Se29 (UPS)KJ850968KJ851108–
Arthonia subfuscicolaThor 11/1 (UPS)KJ850971KJ851110–
Arthonia thoriana 1Sanderson 2176 (BR)MG207687––
Arthonia thoriana 2Sanderson 2174 (herb.Sanderson)MG207685––
Arthothelium orbilliferumTRH-L-15449KY983977––
Arthothelium ruanum 1KoLRI 038018MF616609MF616619–
Arthothelium ruanum 2KoLRI 038261MF616611MF616621–
Arthothelium spectabileFrisch 12Jp179a (TNS)KP870144KP870160–
Chiodecton natalenseErtz 6576 (BR)EU704051EU704014EU704085
Coniocarpon cinnabarinumJohnsen 111003 (UPS)KJ850976KJ851103KJ851083
Coniocarpon fallaxLD:L10075KJ850979KJ851101–
Crypthonia palaeotropicaFrisch 11/Ug457 (UPS)KJ850961KJ851084–
Cryptophaea phaeosporaVan den Broeck 5809 (BR)KX077541––
Cryptothecia austrocoreana 1KolRI No. 041892MF769375––
Cryptothecia austrocoreana 2KolRI No. 044721MF769374––
Cryptothecia bartlettii 1Zhang et al. 20220297 (SDNU)PP051262––
Cryptothecia bartlettii 2Zhang et al. 20220275 (SDNU)PP051261––
Cryptothecia inexspectata 1Liu et al. 20230668 (SDNU)PP051263PP109371–
Cryptothecia inexspectata 2Liu et al. 20230639 (SDNU)PP051264PP109370–
Cryptothecia subnidulans 1v.d.Boom 40613 (hd v.d. Boom)KJ850952KJ851087–
Cryptothecia subnidulans 2Joensson Guyana 6a (UPS)KJ850953KJ851088–
Glomerulophoron mauritiaeErtz 19164 (BR)KP870153KP870167–
Herpothallon inopinatumRudolphi 12 (UPS)KJ850964KJ851099–
Herpothallon kigezienseFrisch 11/Ug26 (UPS)KF707644KF707654–
Herpothallon rubrocinctumRudolphi 5 (UPS)KF707643KF707655–
Herpothallon sp.Frisch 11/Ug401 (UPS)KF707645KF707653–
Inoderma byssaceumThor 25952 (UPS)KJ850962KJ851089KJ851040
Inoderma nipponicumFrisch 12Jp227 (TNS)KP870146KP870162–
Leprantha cinereopruinosaKukwa 17127 & Lubek (BR)MG207692––
Myriostigma candidum 1Ertz 9260 (BR)EU704052EU704015HQ454520
Myriostigma candidum 2Frisch 11/Ug125 (UPS)KJ850959KJ851096–
Myriostigma flavescens 1Liu et al. 20230612 (SDNU)PP051268PP130144–
Myriostigma flavescens 2Liu et al. 20230641 (SDNU)PP051267––
Myriostigma hainana 1Xue et al. 20230061 (SDNU)PP051271PP101845–
Myriostigma hainana 2Xue et al. 20230050 (SDNU)PP051272PP109365–
Myriostigma laxipunctata 1Liu et al. 20231231 (SDNU)PP051266PP109369PP033944
Myriostigma laxipunctata 2Liu et al. 20231052 (SDNU)PP051265PP109368PP033943
Myriostigma miniatumSilva T2A29 (ISE—epitype)KP843606––
Pachnolepia pruinataFrisch 11/Se34 (UPS)KJ850967KJ851098–
Reichlingia leopoldiiErtz 13293JF830773HQ454722HQ454581
Reichlingia syncesioidesFrisch 11/Ug14 (UPS)KF707651KF707656KF707636
Snippocia niveaErtz 17437 (BR)MG207695––
Stirtonia neotropicaCáceres & Aptroot 11112 (ISE)KP843611––
Sporodophoron gossypinumFrisch 12Jp186 (TNS)KP870154KP870168–
Sporodophoron primorskienseOhmura 10509 (TNS)KP870157KP870169–
Synarthonia albopruinosaVDB 6086 (BR<BEL>)MH251873––
Synarthonia inconspicuaVDB 7013B (BR<BEL>)MH251881––
Synarthonia ochraceaVDB 6653 (BR<BEL>)MH251884––
Tylophoron galapagoenseBungartz 8749 (CDS)JF830776–JF295078
Tylophoron hibernicumFrisch 11/Ug220 (UPS)KJ850966KJ851097KJ851065
Tylophoron moderatumErtz 14504 (BR)JF830780–JF295085
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Xue, J.; Cai, Y.; Zhang, L. The Phylogeny and Taxonomy of Cryptothecia (Arthoniaceae, Ascomycota) and Myriostigma (Arthoniaceae, Ascomycota), including Three New Species and Two New Records from China. J. Fungi 2024, 10, 274. https://doi.org/10.3390/jof10040274

AMA Style

Xue J, Cai Y, Zhang L. The Phylogeny and Taxonomy of Cryptothecia (Arthoniaceae, Ascomycota) and Myriostigma (Arthoniaceae, Ascomycota), including Three New Species and Two New Records from China. Journal of Fungi. 2024; 10(4):274. https://doi.org/10.3390/jof10040274

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Xue, Junxia, Yutong Cai, and Lulu Zhang. 2024. "The Phylogeny and Taxonomy of Cryptothecia (Arthoniaceae, Ascomycota) and Myriostigma (Arthoniaceae, Ascomycota), including Three New Species and Two New Records from China" Journal of Fungi 10, no. 4: 274. https://doi.org/10.3390/jof10040274

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