Citrate Regulates the Saccharomyces cerevisiae Mitochondrial GDP/GTP Carrier (Ggc1p) by Triggering Unidirectional Transport of GTP
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Expression and Purification of Wild-Type and Mutant Ggc1p
2.2. Yeast Strains, Media and Growth Conditions
2.3. Reconstitution into Liposomes and Transport Assays
2.4. Comparative Modeling and Docking Investigations
3. Results
3.1. Bacterial Expression of Ggc1p
3.2. Functional Characterization of Recombinant Ggc1p
3.3. Kinetic Characteristics of Recombinant Ggc1p
3.4. Structural Analysis of a Possible Citrate Binding Region on the Ggc1p 3D Model
4. Discussions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Seccia, R.; De Santis, S.; Di Noia, M.A.; Palmieri, F.; Miniero, D.V.; Marmo, R.; Paradies, E.; Santoro, A.; Pierri, C.L.; Palmieri, L.; et al. Citrate Regulates the Saccharomyces cerevisiae Mitochondrial GDP/GTP Carrier (Ggc1p) by Triggering Unidirectional Transport of GTP. J. Fungi 2022, 8, 795. https://doi.org/10.3390/jof8080795
Seccia R, De Santis S, Di Noia MA, Palmieri F, Miniero DV, Marmo R, Paradies E, Santoro A, Pierri CL, Palmieri L, et al. Citrate Regulates the Saccharomyces cerevisiae Mitochondrial GDP/GTP Carrier (Ggc1p) by Triggering Unidirectional Transport of GTP. Journal of Fungi. 2022; 8(8):795. https://doi.org/10.3390/jof8080795
Chicago/Turabian StyleSeccia, Roberta, Silvia De Santis, Maria A. Di Noia, Ferdinando Palmieri, Daniela V. Miniero, Raffaele Marmo, Eleonora Paradies, Antonella Santoro, Ciro L. Pierri, Luigi Palmieri, and et al. 2022. "Citrate Regulates the Saccharomyces cerevisiae Mitochondrial GDP/GTP Carrier (Ggc1p) by Triggering Unidirectional Transport of GTP" Journal of Fungi 8, no. 8: 795. https://doi.org/10.3390/jof8080795