Type 2C Protein Phosphatases MoPtc5 and MoPtc7 Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of Magnaporthe oryzae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Targeted Gene Deletion and Complementation
2.3. Infection Assays, Cuticle Penetration and Incipient Cytorrhysis Assay
2.4. Microscopy
2.5. Southern Blot Analysis, Extraction of Total RNA, qRT-PCR and Gene Expressions
2.6. Bioinformatics Analysis
2.7. Cell Wall Integrity, Osmotic and Oxidative Stresses and Cell Wall Thickness Assays
2.8. Western Blot Assay
2.9. Subcellular Localization Assays
3. Results
3.1. Phylogenetic Analysis of MoPtc5 and MoPtc7
3.2. MoPtc5 and MoPtc7 Are Differentially Expressed during Pathogen-Host Interaction
3.3. MoPtc5 and MoPtc7 Redundantly Contribute to Vegetative Growth of M. oryzae
3.4. MoPtc5 Is Involved in Asexual Reproduction of the Rice Blast Fungus
3.5. MoPtc5 and MoPtc7 Are Involved in Cell Wall Stress Resistance and Dephosphorylation of MoMps1
3.6. MoPtc5 and MoPtc7 May Mediate Chitin Synthesis to Confer Resistance to Cell Wall Stress in the Blast Fungus
3.7. MoPtc5 and MoPtc7 Are Required for Osmotic and Oxidative Stress Tolerance
3.8. MoPtc5 and MoPtc7 Are Responsible for Appressorium Turgor Generation and Degradation of Glycogen in M. oryzae
3.9. MoPtc5 and MoPtc7 Are Involved in M. oryzae Pathogenicity
3.10. Subcellular Localization of MoPtc5 and MoPtc7 Genes
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Biregeya, J.; Anjago, W.M.; Pan, S.; Zhang, R.; Yang, Z.; Chen, M.; Felix, A.; Xu, H.; Lin, Y.; Nkurikiyimfura, O.; et al. Type 2C Protein Phosphatases MoPtc5 and MoPtc7 Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of Magnaporthe oryzae. J. Fungi 2023, 9, 1. https://doi.org/10.3390/jof9010001
Biregeya J, Anjago WM, Pan S, Zhang R, Yang Z, Chen M, Felix A, Xu H, Lin Y, Nkurikiyimfura O, et al. Type 2C Protein Phosphatases MoPtc5 and MoPtc7 Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of Magnaporthe oryzae. Journal of Fungi. 2023; 9(1):1. https://doi.org/10.3390/jof9010001
Chicago/Turabian StyleBiregeya, Jules, Wilfred M. Anjago, Shu Pan, Ruina Zhang, Zifeng Yang, Meilian Chen, Abah Felix, Huxiao Xu, Yaqi Lin, Oswald Nkurikiyimfura, and et al. 2023. "Type 2C Protein Phosphatases MoPtc5 and MoPtc7 Are Crucial for Multiple Stress Tolerance, Conidiogenesis and Pathogenesis of Magnaporthe oryzae" Journal of Fungi 9, no. 1: 1. https://doi.org/10.3390/jof9010001