Inhibitory Mechanisms of trans-2-Hexenal on the Growth of Geotrichum citri-aurantii
Abstract
:1. Introduction
2. Materials and Methods
2.1. Pathogen
2.2. Fruit
2.3. Chemicals
2.4. Antifungal Activity of trans-2-Hexenal against G. citri-aurantii
2.5. In Vivo Experiments of trans-2-Hexenal against G. citri-aurantii
2.6. Scanning Electron Microscopy (SEM) of trans-2-Hexenal against G. citri-aurantii
2.7. Transmission Electron Microscopy (TEM) of trans-2-Hexenal against G. citri-aurantii
2.8. Fourier Transform Infrared (FT−IR) Spectroscopy of trans-2-Hexenal against G. citri-aurantii
2.9. Effect of trans-2-Hexenal on the Cell Wall of G. citri-aurantii
2.10. Effect of trans-2-Hexenal on the Cell Wall of G. citri-aurantii
2.10.1. Effect of trans-2-Hexenal on the Cell Wall Integrity of G. citri-aurantii
2.10.2. Effect of trans-2-Hexenal on the Chitin Content of G. citri-aurantii
2.10.3. Effect of trans-2-Hexenal on the β-1,3-Glucan Content of G. citri-aurantii
2.10.4. Effect of trans-2-Hexenal on the Extracellular Alkaline Phosphatase (AKP) Activities of G. citri-aurantii
2.11. Effect of trans-2-Hexenal on the Cell Membrane of G. citri-aurantii
2.11.1. Effect of trans-2-Hexenal on the Cell Membrane Integrity of G. citri-aurantii
2.11.2. Effect of trans-2-Hexenal on the Total Lipid Content of G. citri-aurantii
2.11.3. Effect of trans-2-Hexenal on the Ergosterol Contents of G. citri-aurantii
2.12. Real-Time Fluorescence Quantitative PCR (RT-qPCR) Analysis
2.13. Molecular Docking
2.14. Statistical Analyses
3. Results
3.1. Antifungal Activity of trans-2-Hexenal against G. citri-aurantii
3.2. In Vivo Experiments of trans-2-Hexenal against G. citri-aurantii
3.3. Scanning Electron Microscopy (SEM) of trans-2-Hexenal against G. citri-aurantii
3.4. Transmission Electron Microscopy (TEM) of trans-2-Hexenal against G. citri-aurantii
3.5. FT−IR of trans-2-Hexenal against G. citri-aurantii
3.6. Effect of trans-2-Hexenal on the Cell Wall of G. citri-aurantii
3.7. Effect of trans-2-Hexenal on the Cell Membrane of G. citri-aurantii
3.8. RT-qPCR
3.9. Molecular Docking
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene ID | Genes | Primer Sequence (5′-3′) |
---|---|---|
CL1729.Contig1_All | FKS1-F | AGGTTGAAGGCAAGCGTACTCT |
FKS1-R | CAGGAAGTGGCTCAGGAATAGGT | |
Unigene4828_All | ERG1-F | AAGTCCTACACCTCCAAGGCTAC |
ERG1-R | GAATATCGGCGTCAGTGAGAACC | |
Unigene3819_All | ERG7-F | TAACGCATATCCAGGACGACCAA |
ERG7-R | CGCACAATCTCAATTCGCTCTTC | |
Unigene3920_All | ERG11-F | CGCCGTAAGGAAGGAAACATTGA |
ERG11-R | AAGACGAAGTAGCAGCCGAAGT | |
CL313.Contig2 | Actin1-F | TTACGCCGGTTTCTCCCTCC |
Actin1-R | GACGATTTCACGCTCGGCAG |
Concentration (µL/mL) | Inhibitory Rate (%) | ||||
---|---|---|---|---|---|
1 d | 2 d | 3 d | 4 d | 5 d | |
0.25 | 100.0 ± 0.0 a | 53.7 ± 5.2 b | 24.8 ± 1.7 c | 27.1 ± 3.0 c | 23.2 ± 1.4 c |
0.50 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 90.5 ± 1.7 b | 72.9 ± 3.9 b | 64.0 ± 8.7 b |
1.00 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a |
2.00 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a |
4.00 | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a | 100.0 ± 0.0 a |
Treatments | Incidence Rate (%) | ||||||
---|---|---|---|---|---|---|---|
1 d | 2 d | 3 d | 4 d | 5 d | 6 d | 7 d | |
Control | 0 ± 0 a | 11 ± 4 a | 22 ± 4 a | 49 ± 4 a | 73 ± 0 a | 89 ± 4 a | 100 ± 0 a |
1× MFC trans-2-hexenal | 0 ± 0 a | 0 ± 0 b | 16 ± 4 b | 22 ± 8 b | 60 ± 7 b | 80 ± 0 b | 85 ± 4 b |
10× MFC trans-2-hexenal | 0 ± 0 a | 0 ± 0 b | 0 ± 0 c | 9 ± 0 c | 22 ± 8 c | 29 ± 8 b | 33 ± 12 c |
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Ouyang, Q.; Shi, S.; Liu, Y.; Yang, Y.; Zhang, Y.; Yuan, X.; Tao, N.; Li, L. Inhibitory Mechanisms of trans-2-Hexenal on the Growth of Geotrichum citri-aurantii. J. Fungi 2023, 9, 930. https://doi.org/10.3390/jof9090930
Ouyang Q, Shi S, Liu Y, Yang Y, Zhang Y, Yuan X, Tao N, Li L. Inhibitory Mechanisms of trans-2-Hexenal on the Growth of Geotrichum citri-aurantii. Journal of Fungi. 2023; 9(9):930. https://doi.org/10.3390/jof9090930
Chicago/Turabian StyleOuyang, Qiuli, Shiwei Shi, Yangmei Liu, Yanqin Yang, Yonghua Zhang, Xingxing Yuan, Nengguo Tao, and Lu Li. 2023. "Inhibitory Mechanisms of trans-2-Hexenal on the Growth of Geotrichum citri-aurantii" Journal of Fungi 9, no. 9: 930. https://doi.org/10.3390/jof9090930