High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges
Abstract
:1. Introduction
2. Results and Discussion
2.1. Size Distribution of Human Mature microRNA
2.2. Isomeric microRNA
2.3. Disease-Associated Isomeric microRNA
2.4. Sequence Similarities among Isomeric microRNA
2.5. Analytical Challenges from Isomeric microRNA
3. Materials and Methods
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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miRNA | Sequence (5′–3′) | Chromosome | Location |
---|---|---|---|
miR-21-5p | UAGCUUAUCAGACUGAUGUUGA | 17 | NC_000017.11 (59841266..59841337) |
miR-95-3p | UUCAACGGGUAUUUAUUGAGCA | 4 | NC_000004.12 (8005301..8005381) |
miR-100-3p | CAAGCUUGUAUCUAUAGGUAUG | 11 | NC_000011.10 (122152229..122152308) |
miR-513b-5p | UUCACAAGGAGGUGUCAUUUAU | X | NC_000023.11 (147199044..147199127) |
miR-519a-3p | AAAGUGCAUCCUUUUAGAGUGU | 19 | NC_000019.10 (53752397..53752481) |
miR-519b-3p | AAAGUGCAUCCUUUUAGAGGUU | 19 | NC_000019.10 (53695213..53695293) |
miR-522-3p | AAAAUGGUUCCCUUUAGAGUGU | 19 | NC_000019.10 (53751211..53751297) |
miR-548am-5p | AAAAGUAAUUGCGGUUUUUGCC | X | NC_000023.11 (16627012..16627085) |
miR-548c-5p | AAAAGUAAUUGCGGUUUUUGCC | 12 | NC_000012.12 (64622509..64622605) |
miR-548h-5p | AAAAGUAAUCGCGGUUUUUGUC | 6 | NC_000006.12 (131792172..131792231) |
miR-548k | AAAAGUACUUGCGGAUUUUGCU | 11 | NC_000011.10 (70283955..70284070) |
miR-548o-5p | AAAAGUAAUUGCGGUUUUUGCC | 20 | NC_000020.11 (38516563..38516632) |
miR-4789-5p | GUAUACACCUGAUAUGUGUAUG | 3 | NC_000003.12 (175369540..175369621) |
miRNA | Sequence (5′–3′) | Chromosome | Location |
---|---|---|---|
let-7a-2-3p | CUGUACAGCCUCCUAGCUUUCC | 11 | NC_000011.10 (122146522..12214659) |
let-7e-3p | CUAUACGGCCUCCUAGCUUUCC | 19 | NC_000019.10 (51692786..51692864) |
miR-301b-3p | CAGUGCAAUGAUAUUGUCAAAGC | 22 | NC_000022.11 (21652981..21653058) |
miR-301a-3p | CAGUGCAAUAGUAUUGUCAAAGC | 17 | NC_000017.11 (59151136..59151221) |
miR-378a-3p | ACUGGACUUGGAGUCAGAAGGC | 5 | NC_000005.10 (149732825..14973289) |
miR-422a | ACUGGACUUAGGGUCAGAAGGC | 15 | NC_000015.10 (63870930..63871019) |
miR-20b-5p | CAAAGUGCUCAUAGUGCAGGUAG | X | NC_000023.11 (134169809..13416987) |
miR-17-5p | CAAAGUGCUUACAGUGCAGGUAG | 13 | NC_000013.11 (91350605..91350688) |
miR-148a-3p | UCAGUGCACUACAGAACUUUGU | 7 | NC_000007.14 (25949919..25949986) |
miR-148b-3p | UCAGUGCAUCACAGAACUUUGU | 12 | NC_000012.12 (54337216..54337314) |
Analytical Method | Specificity for Detecting Specific microRNA | Sample Throughput | Costs | Differentiation of Isomeric microRNA |
---|---|---|---|---|
By Sequencing | ★★★ | Low to High | $$–$$$ | Yes |
By Mass Measurement | ★★★ | Medium | $$ | Size- and Sequence-dependent |
By Complementary Probe | ★★ to ★★★ | Low to High | $$ | Sequence-dependent |
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Mwangi, J.N.; Chiu, N.H.L. High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges. Non-Coding RNA 2016, 2, 13. https://doi.org/10.3390/ncrna2040013
Mwangi JN, Chiu NHL. High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges. Non-Coding RNA. 2016; 2(4):13. https://doi.org/10.3390/ncrna2040013
Chicago/Turabian StyleMwangi, Joseph N., and Norman H. L. Chiu. 2016. "High Percentage of Isomeric Human MicroRNA and Their Analytical Challenges" Non-Coding RNA 2, no. 4: 13. https://doi.org/10.3390/ncrna2040013