Virus-Induced Silencing of a Sequence Coding for Loricrin-like Protein in Phytophthora infestans upon Infection of a Recombinant Vector Based on Tobacco Mosaic Virus
Abstract
:1. Introduction
2. Materials and Methods
2.1. P. infestans Mating Types and Culture Conditions
2.2. TMV-PiLLP-1056 Vector Construction
2.3. Plant Inoculation
2.4. Infection in P. infestans
2.5. Pathogenicity Test
3. Results and Discussion
3.1. P. infestans Mating Types Identification
3.2. Infectivity in Tobacco Plants
3.3. Infectivity in P. infestans
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer Name | Sequence | Size (bp) | Ta | Purpose | Ref. |
---|---|---|---|---|---|
S1a S1b | 5′-AGGATTTCAACAA-3′ 5′-TGCTTCCTAAGG-3′ | 1250 | 50 | Mating type identification | [37] |
PHYB-1 PHYB-2 | 5′GATCGGATTAGTCAGACGAG-3′ 5′-GCGTCTGCAAGGCGCATTTT-3′ | 347 | 59 | Mating type identification | [38] |
W16-1 W16-2 | 5′-AACACGCACAAGGCATATAAATCTA-3′ 5′-GCGTAATGTAGCGTAACAGCTCTC-3′ | 557 | 61 | Mating type identification | [39,40] |
5′UTR for 5′UTR rev | 5′-TCTCCTCGAGTCCTCCTCTCTGCGT-3′ 5′-CGACAATATTCCATAACTGCTAAGGTGGGGCTGTAA-3′ | 201 | 54 | 5′UTR region of PiLLP cloning | This study |
3′UTR for 3′UTR rev | 5′-TATGGAATATTGTCGAGTACAGCAACGAGGCTTCGA-3′ 5′-CCCCCTTAATTAAACGACACTTGAAACATCGTGAG-3′ | 126 | 59 | 3′UTR region of PiLLP cloning | |
5′UTR for 3′UTR rev | 5′-TCTCCTCGAGTCCTCCTCTCTGCGT-3′ 5′-CCCCCTTAATTAAACGACACTTGAAACATCGTGAG-3′ | 327 | 50 | 5′UTR + 3′UTR of PiLLP fusion PCR | |
GFP pro CP pro | 5′-CGATGATGATTCGGAGGCTA-3′ 5′-ATCCAAGACACAACCCTTCG-3′ | ~500 | 55 | pTMV-1056 plasmid sequencing | |
REP for REP rev | 5′-CAGTGCATATCGGCATTGTC-3′ 5′-CGTGACTCCTCTTCCGTCTC-3′ | 241 | 50 | TMV replicase of P. infestans detection | |
PiEF for PiEF rev | 5′-ACTCCAAGAACGACCCTGCTAAGGCAACC-3′ 5′-TCCGACGGCTCGAGGATGACCATGCAG-3′ | 239 | >60 | Real Time PCR of ef1 of P. infestans | [31] |
PiORF for PiORF rev | 5′-CAGTCTCCCGTCACCAGTCC-3′ 5′-GGCAGTCGGCAATACCACAT-3′ | ~300 | >60 | Real Time PCR of PiLLP |
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Labarile, R.; Mincuzzi, A.; Spanò, R.; Mascia, T. Virus-Induced Silencing of a Sequence Coding for Loricrin-like Protein in Phytophthora infestans upon Infection of a Recombinant Vector Based on Tobacco Mosaic Virus. Horticulturae 2023, 9, 360. https://doi.org/10.3390/horticulturae9030360
Labarile R, Mincuzzi A, Spanò R, Mascia T. Virus-Induced Silencing of a Sequence Coding for Loricrin-like Protein in Phytophthora infestans upon Infection of a Recombinant Vector Based on Tobacco Mosaic Virus. Horticulturae. 2023; 9(3):360. https://doi.org/10.3390/horticulturae9030360
Chicago/Turabian StyleLabarile, Rossella, Annamaria Mincuzzi, Roberta Spanò, and Tiziana Mascia. 2023. "Virus-Induced Silencing of a Sequence Coding for Loricrin-like Protein in Phytophthora infestans upon Infection of a Recombinant Vector Based on Tobacco Mosaic Virus" Horticulturae 9, no. 3: 360. https://doi.org/10.3390/horticulturae9030360