Table of Contents
Methods Protoc., Volume 1, Issue 1 (March 2018)
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Cover Story (view full-size image) The generation of CRISPR/Cas9 mutant mice can now be considered routine in many laboratories. [...] Read more. The generation of CRISPR/Cas9 mutant mice can now be considered routine in many laboratories. Pronuclear microinjection is the most commonly used technique to make these mutants due to existing skill sets previously utilised in the generation of overexpression transgenics. However, cytoplasmic microinjection confers some advantages over other techniques such as traditional pronuclear or piezo actuated microinjection. Here, we describe a step-by-step cytoplasmic microinjection protocol using an automatic injector to efficiently generate mouse mutants using CRISPR/Cas9.