Combination of Engineered Expression of Polysialic Acid on Transplanted Schwann Cells and in Injured Rat Spinal Cord Promotes Significant Axonal Growth and Functional Recovery
Abstract
:1. Introduction
2. Materials and Methods
2.1. Production of Lentiviral Vectors and Transduction of Schwann Cells
2.2. Animal Surgery
2.3. Immunohistochemistry
2.4. Behavioral Assessment
2.5. Image Analysis
3. Results
3.1. Lentiviral PST Gene Transfer Induces PSA Expression In Vitro and In Vivo
3.2. Combining PST/SC Transplantation with LV/PST Injection Enhances the Growth of Corticospinal Tract Axons
3.3. Combining PST/SC Transplantation with LV/PST Injection Promotes Locomotor Functional Recovery
3.4. Combining PST/SC Transplantation with LV/PST Injection Does Not Lead to Elevated Pain Sensation
3.5. PST/SCs Maintain Their Capacity to Myelinate Axons
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gao, F.; Zhang, Y.; Wu, D.; Luo, J.; Gushchina, S.; Bo, X. Combination of Engineered Expression of Polysialic Acid on Transplanted Schwann Cells and in Injured Rat Spinal Cord Promotes Significant Axonal Growth and Functional Recovery. Neuroglia 2023, 4, 222-238. https://doi.org/10.3390/neuroglia4040016
Gao F, Zhang Y, Wu D, Luo J, Gushchina S, Bo X. Combination of Engineered Expression of Polysialic Acid on Transplanted Schwann Cells and in Injured Rat Spinal Cord Promotes Significant Axonal Growth and Functional Recovery. Neuroglia. 2023; 4(4):222-238. https://doi.org/10.3390/neuroglia4040016
Chicago/Turabian StyleGao, Fangyou, Yi Zhang, Dongsheng Wu, Juan Luo, Svetlana Gushchina, and Xuenong Bo. 2023. "Combination of Engineered Expression of Polysialic Acid on Transplanted Schwann Cells and in Injured Rat Spinal Cord Promotes Significant Axonal Growth and Functional Recovery" Neuroglia 4, no. 4: 222-238. https://doi.org/10.3390/neuroglia4040016