Aging-Related Changes in Expression and Function of Glutamate Transporters in Rat Spinal Cord Astrocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Experimental Animals
2.3. Western Blotting
2.4. Immunohistochemistry
2.5. Quantification of Receptor Expression
2.6. Quantification of Astrocytic Numerical Density and Coverage
2.7. Acute Slice Preparation for Whole-Cell Patch-Clamp Recording
2.8. Statistical Analysis
3. Results
3.1. Distinct Density and Morphology of Astrocytes in DH and VH of Rat Spinal Cord and Changes with Age
3.2. Change in Expression of Astrocytic Glutamate Transporters EAAT1 and EAAT2 in the Spinal Cord with Age
3.3. Glutamate Uptake in DH and VH Astrocytes with Aging
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Sharan, S.; Tewari, B.P.; Joshi, P.G. Aging-Related Changes in Expression and Function of Glutamate Transporters in Rat Spinal Cord Astrocytes. Neuroglia 2023, 4, 290-306. https://doi.org/10.3390/neuroglia4040020
Sharan S, Tewari BP, Joshi PG. Aging-Related Changes in Expression and Function of Glutamate Transporters in Rat Spinal Cord Astrocytes. Neuroglia. 2023; 4(4):290-306. https://doi.org/10.3390/neuroglia4040020
Chicago/Turabian StyleSharan, Shiksha, Bhanu Prakash Tewari, and Preeti G. Joshi. 2023. "Aging-Related Changes in Expression and Function of Glutamate Transporters in Rat Spinal Cord Astrocytes" Neuroglia 4, no. 4: 290-306. https://doi.org/10.3390/neuroglia4040020