Antibacterial Action, Antioxidant Activity and Anticoagulant Effect of Pectin Extracted from Peels of Algerian Citrus Sinensis †
Abstract
:1. Introduction
2. Materials and Methods
2.1. Extraction of Pectin
2.1.1. Extraction with Citric Acid
2.1.2. Extraction with Hydrochloric Acid
3. Results and Discussion
3.1. Yield of Pectin
3.2. Degree of Methylesterification (DE)
3.3. FTIR Characterisation
3.4. XRD Characterisation
3.5. Antibacterial Activity
3.6. Antioxidant Activity
3.7. Anticoagulant Activity
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
- Anwar, F.; Naseer, R.; Bhanger, M.I.; Ashraf, S.; Talpur, F.N.; Aladedunye, F.A. Physico-chemical characteristics of citrus seeds and seed oils from Pakistan. J. Am. Oil Chem. Soc. 2008, 85, 321–330. [Google Scholar] [CrossRef]
- Fisher, K.; Phillips, C. Potential antimicrobial uses of essential oils in food: Is citrus the answer? Trend Food Sci. Technol. 2008, 19, 156–164. [Google Scholar] [CrossRef]
- Pandharipande, S.; Makode, H. Separation of oil and pectin from orange peel and study of effect of pH of extracting medium on the yield of pectin. J. Eng. Res. Stud. 2012, 3, 6–9. [Google Scholar]
- Bonnin, E.; Garnier, C.; Ralet, M.C. Pectin-modifying enzymes and pecderived materials: Applications and impacts. Appl. Microbiol. Biotechnol. 2014, 98, 519–532. [Google Scholar] [CrossRef] [PubMed]
- Espinal-Ruiz, M.; Restrepo-Sánchez, L.P.; Narváez-Cuenca, C.E.; McClements, D.J. Impact of pectin properties on lipid digestion under simulated gastrointestinal conditions: Comparison of citrus and banana passion fruit (Passiflora tripartita var. mollissima) pectins. Food Hydrocoll. 2016, 52, 329–342. [Google Scholar] [CrossRef]
- Salas, M.P.; Céliz, G.; Geronazzo, H.; Daz, M.; Resnik, S.L. Antifungal activity of natural and enzymatically-modified flavonoids isolated from citrus species. Food Chem. 2011, 124, 1411–1415. [Google Scholar] [CrossRef]
- Liew, S.Q.; Chin, N.L.; Yusof, Y.A. Extraction and characterization of pect from passion fruit peels. Agric. Agric. Sci. Procedia 2014, 2, 231–236. [Google Scholar]
- Alok, K.T.; Samarendra, N.S.; Vishnu, P.Y.; Uttam, K.U.; Deepshikha, K.; Tanya, M. Extraction and Characterization of Pectin from Orange Peels. Int. J. Biotechnol. Biochem. 2017, 13, 39–47. [Google Scholar]
- Kratchanova, M.; Pavlova, E.; Panchev, I. The effect of microwave heating of fresh orange peels on the fruit tissue and quality of extracted pectin. Carbohydr. Polym. 2004, 56, 181–185. [Google Scholar] [CrossRef]
- Yu, Y.; Wang, Y.; Liu, X.; Liu, Y.; Ji, L.; Zhou, Y.; Sun, L. Comparison of Analytical Methods for Determining Methylesterification and Acetylation of Pectin. Appl. Sci. 2021, 11, 4461. [Google Scholar] [CrossRef]
- Canteri-Schemin, M.H.; Fertonani, H.C.R.; Waszczynskyj, N.; Wosiacki, G. Extraction of Pectin from Apple Pomace. Braz. Arch. Biol. Technol. 2005, 48, 259–266. [Google Scholar] [CrossRef]
- Yapo, B.M.; Robert, C.; Etienne, I.; Wathelet, B.; Paquot, M. Effect of Extraction Conditions on the Yield, Purity and Surface Properties of Sugar Beet Pulp Pectin Extracts. J. Food Chem. 2007, 100, 1356–1364. [Google Scholar] [CrossRef]
- Ye, S.; Zhu, Z.; Wen, Y.; Su, C.; Jiang, L.; He, S.; Shao, W. Facile and green preparation of pectin/cellulose composite films with enhanced antibacterial and antioxidant behaviors. Polymers 2019, 11, 57. [Google Scholar] [CrossRef] [PubMed]
- Einhorn-Stoll, U.; Kunzek, H. Thermoanalytical characterisation of processing-dependent structural changes and state transitions of citrus pectin. Food Hydrocoll. 2009, 23, 40–52. [Google Scholar] [CrossRef]
- Boudouaia, N.; Benine, M.L.; Fettal, N.; Abbouni, B.; Bengharez, Z. Antibacterial Action of Chitosan Produced from Shrimp Waste Against the Growth of Escherichia coli, Staphylococcus epidermidis, Proteus mirabilis and Pseudomonas aeruginosa. Waste Biomass Valorization 2023. [Google Scholar] [CrossRef]
- Wong, S.P.; Leong, L.P.; Koh, J.H.W. Antioxidant activities of aqueous extracts of selected plants. Food Chem. 2006, 99, 775–783. [Google Scholar] [CrossRef]
- Yoon, S.J.; Pereira, M.S.; Pavao, M.S.G.; Hwang, J.K.; Pynu, Y.R.; Mourao, P.A.S. The medicinal plant Poranavolubilis contains polysaccharides with anticoagulant activity mediated by heparin cofactor II. Thromb. Res. 2002, 106, 51–58. [Google Scholar] [CrossRef] [PubMed]
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Boudouaia, N.; Bendaoudi, A.A.; Benykhlef, S.; Bengharez, Z.; Cantarero, A. Antibacterial Action, Antioxidant Activity and Anticoagulant Effect of Pectin Extracted from Peels of Algerian Citrus Sinensis. Chem. Proc. 2023, 14, 69. https://doi.org/10.3390/ecsoc-27-16069
Boudouaia N, Bendaoudi AA, Benykhlef S, Bengharez Z, Cantarero A. Antibacterial Action, Antioxidant Activity and Anticoagulant Effect of Pectin Extracted from Peels of Algerian Citrus Sinensis. Chemistry Proceedings. 2023; 14(1):69. https://doi.org/10.3390/ecsoc-27-16069
Chicago/Turabian StyleBoudouaia, Nacer, Ahmed Amine Bendaoudi, Samir Benykhlef, Zohra Bengharez, and Andres Cantarero. 2023. "Antibacterial Action, Antioxidant Activity and Anticoagulant Effect of Pectin Extracted from Peels of Algerian Citrus Sinensis" Chemistry Proceedings 14, no. 1: 69. https://doi.org/10.3390/ecsoc-27-16069