Mapping Single Nucleotide Polymorphism Markers Associated with the Pre-Flowering Morphological Performance of Fenugreek under Different Levels of Salt Stress
Abstract
:1. Introduction
2. Results
Association Mapping
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Material and Experimental Design
5.2. Phenotypic Measurements
5.3. Statistical Analysis
5.4. SNP Data and Mapping
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Traits (Unit) | Control | 3000 ppm | 6000 ppm | MM | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Min | Max | Avg | SE | H2 | Min | Max | Avg | SE | H2 | Min | Max | Avg | SE | H2 | T | G | GxT | |
LN | 19.85 | 22.00 | 20.93 | 0.49 | 0.87 | 26.80 | 29.03 | 27.92 | 0.52 | 0.69 | 27.28 | 29.50 | 28.39 | 0.52 | 0.51 | 0.00 | 0.00 | 0.00 |
BN | 1.78 | 2.27 | 2.03 | 0.09 | 0.43 | 1.95 | 2.43 | 2.19 | 0.10 | 0.44 | 2.56 | 3.04 | 2.80 | 0.10 | 0.44 | 0.00 | 0.00 | 0.00 |
PH (cm) | 15.52 | 16.40 | 15.96 | 0.22 | 0.78 | 15.78 | 16.71 | 16.25 | 0.24 | 0.55 | 15.78 | 16.71 | 16.25 | 0.24 | 0.52 | 0.04 | 0.00 | 0.00 |
FW (g) | 1.05 | 1.49 | 1.27 | 0.11 | 0.56 | 0.86 | 1.33 | 1.08 | 0.12 | 0.67 | 0.87 | 1.32 | 1.09 | 0.12 | 0.52 | 0.50 | 0.00 | 0.00 |
DW (g) | 0.32 | 0.81 | 0.56 | 0.13 | 0.49 | 0.37 | 0.90 | 0.63 | 0.13 | 0.46 | 0.70 | 1.20 | 0.95 | 0.13 | 0.42 | 0.08 | 0.00 | 0.00 |
RD (cm) | 12.53 | 14.30 | 13.41 | 0.33 | 0.68 | 11.76 | 13.51 | 12.64 | 0.35 | 0.55 | 12.87 | 14.63 | 13.75 | 0.34 | 0.43 | 0.00 | 0.00 | 0.00 |
Trait | Marker | −Log10 (p) | R2 | Allele | Effect |
---|---|---|---|---|---|
LN_C | dDocent_Contig_30812_276 | 4.0 | 0.16 | A(G) | −10.21 |
LN_C | dDocent_Contig_45644_257 | 4.0 | 0.16 | A(C) | 9.21 |
LN_3000 | dDocent_Contig_9350_202 | 4.1 | 0.20 | G(T) | −18.12 |
LN_6000 | dDocent_Contig_31894_14 | 4.0 | 0.18 | C(T) | 16.03 |
LN_6000 | dDocent_Contig_47529_298 | 4.9 | 0.22 | A(G) | −34.51 |
BN_6000 | dDocent_Contig_1933_583 | 4.1 | 0.18 | A(G) | 6.25 |
BN_6000 | dDocent_Contig_1933_593 | 4.1 | 0.18 | C(T) | −6.25 |
BN_6000 | dDocent_Contig_29601_61 | 4.0 | 0.18 | C(T) | 6.73 |
BN_6000 | dDocent_Contig_29746_105 | 4.0 | 0.23 | G(T) | 16.60 |
BN_6000 | dDocent_Contig_29746_130 | 4.0 | 0.23 | C(G) | 16.60 |
BN_6000 | dDocent_Contig_29746_136 | 4.0 | 0.23 | C(G) | 16.60 |
BN_6000 | dDocent_Contig_29746_145 | 4.0 | 0.23 | C(G) | 16.60 |
BN_6000 | dDocent_Contig_29746_24 | 4.0 | 0.23 | C(T) | 16.60 |
BN_6000 | dDocent_Contig_29746_254 | 4.0 | 0.23 | C(G) | 16.60 |
BN_6000 | dDocent_Contig_29746_281 | 4.0 | 0.23 | A(G) | 16.60 |
BN_6000 | dDocent_Contig_29746_7 | 4.0 | 0.23 | A(G) | 16.60 |
BN_6000 | dDocent_Contig_29746_96 | 4.0 | 0.23 | C(G) | 16.60 |
BN_6000 | dDocent_Contig_6519_195 | 4.0 | 0.18 | C(T) | −5.39 |
BN_6000 | dDocent_Contig_797_289 | 4.9 | 0.23 | A(G) | −8.46 |
PH_C | dDocent_Contig_373_122 | 4.0 | 0.17 | C(T) | −3.69 |
PH_3000 | dDocent_Contig_40266_46 | 4.4 | 0.21 | A(G) | 4.46 |
PH_6000 | dDocent_Contig_6598_111 | 4.0 | 0.18 | G(T) | 4.61 |
FW_6000 | dDocent_Contig_31699_109 | 4.1 | 0.18 | A(G) | 1.37 |
FW_6000 | dDocent_Contig_31699_114 | 4.1 | 0.18 | C(G) | 1.37 |
FW_6000 | dDocent_Contig_31699_37 | 4.1 | 0.18 | C(T) | 1.37 |
FW_6000 | dDocent_Contig_31699_8 | 4.1 | 0.18 | A(G) | −1.37 |
WC_C | dDocent_Contig_3179_151 | 4.8 | 0.21 | C(G) | −0.07 |
RD_C | dDocent_Contig_53508_178 | 4.5 | 0.19 | A(T) | −2.11 |
Trait | Marker | −Log10 (p) | R2 | Allele | Effect |
---|---|---|---|---|---|
FW/DW_C | dDocent_Contig_30841_53 | 4.2 | 0.17 | C(T) | 2.01 |
4 | 0.17 | C(T) | 4.22 | ||
dDocent_Contig_31124_100 | 4.3 | 0.18 | C(T) | −1.88 | |
4.2 | 0.18 | C(T) | −3.98 | ||
dDocent_Contig_31124_249 | 4.7 | 0.2 | A(G) | −1.98 | |
4.6 | 0.2 | A(G) | −4.25 | ||
dDocent_Contig_31124_47 | 4.8 | 0.21 | A(T) | 2.19 | |
4.6 | 0.2 | A(T) | 4.64 | ||
dDocent_Contig_35845_179 | 3.9 | 0.16 | A(G) | −1.93 | |
4 | 0.16 | A(G) | −4.19 | ||
dDocent_Contig_35845_189 | 3.9 | 0.16 | A(G) | −1.93 | |
4 | 0.16 | A(G) | −4.19 | ||
dDocent_Contig_36571_20 | 4 | 0.16 | A(T) | −0.12 | |
3.5 | 0.14 | A(T) | −0.37 | ||
dDocent_Contig_43254_163 | 5.2 | 0.23 | C(T) | −2.56 | |
4.9 | 0.22 | C(T) | −5.36 | ||
dDocent_Contig_43254_80 | 5 | 0.22 | G(T) | −2.51 | |
4.8 | 0.21 | G(T) | −5.3 | ||
dDocent_Contig_49746_342 | 4 | 0.21 | A(G) | −4.6 | |
4.1 | 0.22 | A(G) | −10.2 | ||
dDocent_Contig_5198_91 | 4.4 | 0.19 | A(G) | −2.08 | |
4.7 | 0.2 | A(G) | −4.66 | ||
dDocent_Contig_57115_226 | 3.9 | 0.2 | A(T) | −4.9 | |
4.9 | 0.26 | A(T) | −13.6 | ||
dDocent_Contig_6156_243 | 3.6 | 0.14 | A(G) | −1.32 | |
4 | 0.16 | A(G) | −3.04 | ||
dDocent_Contig_63002_15 | 4.3 | 0.22 | C(T) | −4.90 | |
4.1 | 0.22 | C(T) | −10.30 | ||
FW/DW_3000 | dDocent_Contig_35199_79 | 3 | 0.1323 | G(T) | −0.20 |
3 | 0.13144 | G(T) | −0.34 | ||
dDocent_Contig_64264_238 | 3.7 | 0.17492 | A(G) | −0.23 | |
3 | 0.1342 | A(G) | −0.33 | ||
dDocent_Contig_64858_150 | 3.3 | 0.1699 | C(G) | −0.24 | |
3.5 | 0.18123 | C(G) | −0.43 | ||
dDocent_Contig_85747_243 | 3.7 | 0.1758 | C(T) | −0.24 | |
3.6 | 0.1653 | C(T) | −0.38 | ||
dDocent_Contig_9501_294 | 3.2 | 0.14269 | A(C) | 0.26 | |
3.1 | 0.13883 | A(C) | 0.43 | ||
dDocent_Contig_95794_210 | 3.4 | 0.15403 | A(C) | 0.46 | |
3.7 | 0.17421 | A(C) | 0.82 | ||
FW/DW_6000 | dDocent_Contig_61819_183 | 3.3 | 0.16 | C(T) | −0.35 |
4.2 | 0.22 | C(T) | −0.71 | ||
dDocent_Contig_58866_61 | 4 | 0.18 | C(T) | 0.36 | |
4.2 | 0.19 | C(T) | 0.65 | ||
dDocent_Contig_39980_178 | 4.6 | 0.21 | A(G) | −0.52 | |
4.1 | 0.18 | A(G) | −0.87 | ||
dDocent_Contig_10975_317 | 3.7 | 0.16 | G(T) | 0.71 | |
5.1 | 0.24 | G(T) | 1.52 | ||
dDocent_Contig_12419_342 | 3.4 | 0.14 | A(T) | −0.69 | |
4.9 | 0.23 | A(T) | −1.54 | ||
dDocent_Contig_31895_105 | 4.1 | 0.18 | C(T) | −0.73 | |
3.7 | 0.16 | C(T) | −1.2 |
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Dobeie, A.M.; Nemr, R.A.; Abd El-Wahab, M.M.H.; Shahba, M.; El-Soda, M. Mapping Single Nucleotide Polymorphism Markers Associated with the Pre-Flowering Morphological Performance of Fenugreek under Different Levels of Salt Stress. Stresses 2024, 4, 282-292. https://doi.org/10.3390/stresses4020017
Dobeie AM, Nemr RA, Abd El-Wahab MMH, Shahba M, El-Soda M. Mapping Single Nucleotide Polymorphism Markers Associated with the Pre-Flowering Morphological Performance of Fenugreek under Different Levels of Salt Stress. Stresses. 2024; 4(2):282-292. https://doi.org/10.3390/stresses4020017
Chicago/Turabian StyleDobeie, Amani Mahmoud, Rahma A. Nemr, Mustafa M. H. Abd El-Wahab, Mohamed Shahba, and Mohamed El-Soda. 2024. "Mapping Single Nucleotide Polymorphism Markers Associated with the Pre-Flowering Morphological Performance of Fenugreek under Different Levels of Salt Stress" Stresses 4, no. 2: 282-292. https://doi.org/10.3390/stresses4020017