Enhancement of Catalytic Efficiency of Enzymatic Redox Reactions by Composing Horseradish Peroxidase-Modified Electrode with Ionic Liquids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation of HRP-Modified Electrode
2.3. Instruments
3. Results
3.1. Chronoamperometric Response Performance at the HRP/CP-Modified Electrode under Applied Potential Control
3.2. Current Behavior of the HRP/CP/EC/MEMPTFSI-Modified Electrode by Injecting H2O2
3.3. Kinetic Behavior of the HRP/CP/EC/MEMPTFSI-Modified Electrode
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Enzyme-Modified Electrode | KM (M) | Vmax (μM−1s−1) | kcat (s−1) | kcat/KM (M−1s−1) |
---|---|---|---|---|
HRP/CP-modified electrode | 0.09 | 0.50 | 0.06 | 0.67 |
HRP/CP/EC/MEMPTFSI-modified electrode | 0.09 | 2.84 | 0.36 | 4.00 |
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Noritomi, Y.; Kuboki, T.; Noritomi, H. Enhancement of Catalytic Efficiency of Enzymatic Redox Reactions by Composing Horseradish Peroxidase-Modified Electrode with Ionic Liquids. Liquids 2024, 4, 393-401. https://doi.org/10.3390/liquids4020020
Noritomi Y, Kuboki T, Noritomi H. Enhancement of Catalytic Efficiency of Enzymatic Redox Reactions by Composing Horseradish Peroxidase-Modified Electrode with Ionic Liquids. Liquids. 2024; 4(2):393-401. https://doi.org/10.3390/liquids4020020
Chicago/Turabian StyleNoritomi, Yasuko, Takashi Kuboki, and Hidetaka Noritomi. 2024. "Enhancement of Catalytic Efficiency of Enzymatic Redox Reactions by Composing Horseradish Peroxidase-Modified Electrode with Ionic Liquids" Liquids 4, no. 2: 393-401. https://doi.org/10.3390/liquids4020020