sensors-logo

Journal Browser

Journal Browser

Biosensors for Low Abundant Molecules

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (30 November 2018) | Viewed by 6443

Special Issue Editor


E-Mail Website
Guest Editor
Institute of Applied Sciences and Intelligent Systems “Eduardo Caianiello” of the National Research Council of Italy; CNR-ISASI, Pozzuoli, Italy
Interests: biosensors for low abundant biomarkers; pyroelectric effect; ferroelectric crystals; electrohydrodynamics; inkjet printing; bio-engineering; electrospinning; surface wettability; soft matter manipulation; biofilm sensing

Special Issue Information

Dear Colleagues,

The development of biosensors that are able to detect low abundant molecules is of paramount importance in all of cases where only highly-diluted solutions of a sample are available. This may occur in a wide variety of fields, such as in biomedicine, when a few molecules of biomarkers have to be detected in human fluids in the early stage of a disease, as well as in forensic and security applications, where traces of human DNA or of dangerous chemicals have to be recognized with high sensitivity.

This Special Issue focuses on research and development of biosensors as analytical tools for detecting molecules with sensitivities well beyond those available nowadays through well-established techniques. Contributions presenting different techniques, materials and configurations, which aim at developing high sensitive biosensors, are very welcome. Even researchers studying the theoretical aspects related to diffusion limits in immunoreaction protocols are encouraged to present their recent advances in this special issue.

The applications may cover many areas, including biomedicine, environmental sensing, security, forensic, and all of those case studies where low abundant molecules have to be detected. The accepted works will include seminal demonstrations of new methods, as well as mature procedures to be made available on the market.

Both review articles and original research papers are welcome.

Dr. Simonetta Grilli
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Sensors is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensors
  • biomarkers
  • low abundant molecules
  • portable biosensors
  • immunoassays
  • enzyme-linked immunosorbent assay (ELISA)
  • proteins
  • DNA
  • antibody
  • non invasive clinical assays
  • lab-on-a-chip
  • small sample volumes
  • biological fluids
  • environmental control
  • super-hydrophobic surfaces
  • diffusion limits
  • plasmonic surfaces
  • electrodynamics
  • Raman spectroscopy
  • nanotechnology
  • nanoparticles
  • nano-matrials
  • nanoptohonics

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Review

13 pages, 6069 KiB  
Review
The Applications of Promoter-gene-Engineered Biosensors
by Yingzhu Feng, Zhangzhang Xie, Xuanlong Jiang, Zhen Li, Yuping Shen, Bochu Wang and Jianzhong Liu
Sensors 2018, 18(9), 2823; https://doi.org/10.3390/s18092823 - 27 Aug 2018
Cited by 12 | Viewed by 6101
Abstract
A promoter is a small region of a DNA sequence that responds to various transcription factors, which initiates a particular gene expression. The promoter-engineered biosensor can activate or repress gene expression through a transcription factor recognizing specific molecules, such as polyamine, sugars, lactams, [...] Read more.
A promoter is a small region of a DNA sequence that responds to various transcription factors, which initiates a particular gene expression. The promoter-engineered biosensor can activate or repress gene expression through a transcription factor recognizing specific molecules, such as polyamine, sugars, lactams, amino acids, organic acids, or a redox molecule; however, there are few reported applications of promoter-enhanced biosensors. This review paper highlights the strategies of construction of promoter gene-engineered biosensors with human and bacteria genetic promoter arrays with regard to high-throughput screening (HTS) molecular drugs, the study of the membrane protein’s localization and nucleocytoplasmic shuttling mechanism of regulating factors, enzyme activity, detection of the toxicity of intermediate chemicals, and probing bacteria density to improve value-added product titer. These biosensors’ sensitivity and specificity can be further improved by the proposed approaches of Mn2+ and Mg2+ added random error-prone PCR that is a technique used to generate randomized genomic libraries and site-directed mutagenesis approach, which is applied for the construction of bacteria’s “mutant library”. This is expected to establish a flexible HTS platform (biosensor array) to large-scale screen transcription factor-acting drugs, reduce the toxicity of intermediate compounds, and construct a gene-dynamic regulatory system in “push and pull” mode, in order to effectively regulate the valuable medicinal product production. These proposed novel promoter-engineered biosensors aiding in synthetic genetic circuit construction will maximize the efficiency of the bio-synthesis of medicinal compounds, which will greatly promote the development of microbial metabolic engineering and biomedical science. Full article
(This article belongs to the Special Issue Biosensors for Low Abundant Molecules)
Show Figures

Figure 1

Back to TopTop