Complete Genome Sequence of Two Deep-Sea Streptomyces Isolates from Madeira Archipelago and Evaluation of Their Biosynthetic Potential
Abstract
:1. Introduction
2. Results
2.1. Isolation, Phenotypic Characterization and Sequencing
2.2. Genome Assembly and Annotation
2.3. Phylogenetic Analysis of the Deep-Sea Isolated Strains
2.4. Marine Adaptation Genes
2.5. Secondary Metabolism in Silico profiling
3. Discussion
4. Materials and Methods
4.1. Sampling, Isolation and Microbial Growth
4.2. Genomic DNA Isolation and PCR Amplification
4.3. Short-Read (Illumina) and Long-Read (PacBio) Sequencing
4.4. De Novo Genome Assembly and Annotation
4.5. Identification of Putative Marine Adaptation Genes
4.6. Phylogenetic Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate | Genome Size (bp) | Fold Coverage (x) | G+C Content (%) | No. of CDS 1 | No. of rRNA Operons | No. of tRNA Genes | No. of BGCs 2 | GenBank Accession Number |
---|---|---|---|---|---|---|---|---|
MA3_2.13 | 7,653,710 | 139 | 72.1 | 6412 | 5 | 55 | 32 | CP082362 |
S07_1.15 | 7,094,148 | 159 | 73.2 | 6492 | 6 | 62 | 24 | JAJBZK000000000 |
160,397 |
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Albuquerque, P.; Ribeiro, I.; Correia, S.; Mucha, A.P.; Tamagnini, P.; Braga-Henriques, A.; Carvalho, M.d.F.; Mendes, M.V. Complete Genome Sequence of Two Deep-Sea Streptomyces Isolates from Madeira Archipelago and Evaluation of Their Biosynthetic Potential. Mar. Drugs 2021, 19, 621. https://doi.org/10.3390/md19110621
Albuquerque P, Ribeiro I, Correia S, Mucha AP, Tamagnini P, Braga-Henriques A, Carvalho MdF, Mendes MV. Complete Genome Sequence of Two Deep-Sea Streptomyces Isolates from Madeira Archipelago and Evaluation of Their Biosynthetic Potential. Marine Drugs. 2021; 19(11):621. https://doi.org/10.3390/md19110621
Chicago/Turabian StyleAlbuquerque, Pedro, Inês Ribeiro, Sofia Correia, Ana Paula Mucha, Paula Tamagnini, Andreia Braga-Henriques, Maria de Fátima Carvalho, and Marta V. Mendes. 2021. "Complete Genome Sequence of Two Deep-Sea Streptomyces Isolates from Madeira Archipelago and Evaluation of Their Biosynthetic Potential" Marine Drugs 19, no. 11: 621. https://doi.org/10.3390/md19110621