Synthesis, Characterization and Antifungal Evaluation of 5-Substituted-4-Amino-1,2,4-Triazole-3-Thioesters

Abstract

A series of 5-substituted-4-amino-1,2,4-triazole-3-thioesters was synthesized by converting variously substituted organic acids successively into the corresponding esters, hydrazides, 5-substituted-1,3,4-oxadiazole-2-thiols, 5-substituted-1,2,4-triazole-2-thiols and 5-substituted-1,3,4-oxadiazole-2-thioesters. Finally the target compounds were obtained by refluxing 5-substituted-1,3,4-oxadiazole-2-thioesters in the presence of hydrazine hydrate and absolute alcohol. The structures of the synthesized compounds were established by physicochemical and spectroscopic methods. The synthesized compounds were evaluated for their in vitro antifungal activity. Some of the evaluated compounds possessed significant antifungal activity as compared to a terbinafine standard.

1. Introduction

1,2,4-Triazoles and their derivatives constitute an important class of organic compounds with diverse agricultural, industrial and biological activities [1,2,3] including anti-microbial [4,5] sedative, anti-convulsant [6] and anti-inflammatory properties [7], and consequently, the synthesis of these heterocycles has received considerable attention in recent years [8,9,10,11,12,13,14]. In this work we report the synthesis of some new 5-substituted-4-amino-1,2,4-triazole-3-thioesters through the intermolecular cyclization of variably substituted acid hydrazides to the corresponding 5-substituted-1,3,4-oxadiazoles-2-thioles, 5-substituted-1,2,4-triazoles-2-thioles and 5-substituted-1,3,4-oxadiazoles-2-thioesters.

2. Results and Discussion

Nine 5-substituted-4-amino-1,2,4-triazole-3-thioesters were obtained in 66-83% yield by converting variously substituted organic acids Ra-i to the corresponding esters 1a-i and acid hydrazides 2a-i by reaction with methanol and hydrazine hydrate, respectively. The hydrazides were converted to 5-substituted-1,3,4-oxadiazole-2-thiols 3a-i and 5-substituted-1,2,4-triazole-2-thiols 4a-i as described in [15,16]. 5-Substituted-1,3,4-oxadiazole-2-thiols and 5-substituted-1,2,4-triazole-2-thiols were next converted to 5-substituted-1,3,4-oxadiazole-2-thioesters 5a-i using potassium hydroxide and acid chlorides and finally to 5-substituted-4-amino-1,2,4-triazole-3-thioesters 6a-i by refluxing the 5-substituted-1,3,4-oxadiazole-2-thioesters in the presence of hydrazine hydrate and absolute ethanol, as outlined in Scheme 1.

Mass spectral data support the proposed structures. Scheme 2 shows the fragmentation pattern for 5-(4-methoxybenzyl)-4-amino-1,2,4-triazole-2-ylnapthalene-1-carbothioate (6a) which serves as an example for all the compounds mutatis mutandis. The mass spectrum showed various characteristic peaks. A peak at m/z 390 was assigned to the molecular ion. A peak at m/z 155 (the base peak) was due to the naphthoyl fragment and a characteristic peak at m/z 121 was attributed to the tropylium ion produced by the loss of triazole-3-thioester moiety.

The FTIR spectrum showed absorption bands at 1,518 cm^–1, 3,204 cm^–1, 1,684 cm^–1 and 1,575 cm^-1 which were assigned to C=N, N–H, C=O (ester), C–O and C=C (aromatic), respectively. The ¹H-NMR spectrum of 6a showed characteristic signals at 6.90 to 7.85 ppm which were assigned to the aromatic protons. A signal at 2.50 ppm was assigned to the methylene proton. The singlet at 3.56 and 3.83 ppm were assigned to the methoxy and amino protons, respectively.

The ¹³C-NMR spectrum of 6a showed characteristic peaks at 120.5-134.5 ppm which were assigned to the aromatic carbon atoms. A peak at 180.01 ppm was attributed to the carbonyl carbon atom. The peaks at 166.04 and 175.10 ppm were assigned to the carbon atoms of the triazole moiety. The peaks at 30.6 and 55.9 ppm were assigned to the methylene and methoxy carbon atoms. The significant ¹³C-NMR spectra signals are listed in

Table 2. ¹³C-NMR spectral data of 5-substitued-4-amino-1,2,4-triazole-3-thioesters 6a-i.

Carbon No.	13C-δ(ppm), Compounds
	6a	6b	6c	6d	6e	6f	6g	6h	6i
1	129.41	128.40	130.05	28.36	30.33	130.06	30.60	130.46	133.06
2	128.40	127.41	126.41	30.18	32.71	127.25	55.90	125.34	127.45
3	116.52	155.50	116.24	127.21	130.43	152.44	130.02	116.73	153.44
4	115.52	116.62	115.24	126.33	118.23	127.25	127.05	118.62	125.21
5	116.31	118.62	116.37	127.41	117.23	126.31	116.65	116.73	127.40
6	130.43	127.42	126.21	116.24	115.48	117.21	158.03	125.23	117.67
7	160.42	162.61	166.04	118.44	118.23	161.37	166.04	159.68	163.23
8	171.40	174.65	176.23	116.26	125.63	170.68	175.10	168.77	177.45
9	182.31	180.37	184.32	166.23	159.21	182.04	180.01	184.66	181.08
10	135.61	136.23	134.05	175.90	170.62	54.34	116.65	53.62	136.38
11	127.12	129.12	130.73	182.64	183.47	44.31	127.05	44.02	127.21
12	120.43	122.31	126.32	45.46	134.24	33.39	134.50	34.71	116.90
13	122.41	125.71	125.61	33.67	128.40	24.36	127.12	29.01	126.64
14	125.32	128.81	126.67	26.34	129.65	25.62	125.62	25.72	128.03
15	126.32	127.50	130.73	25.80	126.31	20.22	120.50	25.69	125.64
16	128.43	130.64	–	20.21	130.52	–	119.42	20.02	–
17	129.42	132.80	–	18.44	127.43	–	129.31	18.33	–
18	130.21	126.21	–	22.37	–	–	131.61	–	–
19	129.66	129.44	–	–	–	–	130.71	–	–
20	–	–	–	–	–	–	123.63	–	–

and are in agreement with the proposed structure. On the basis of the combined physical and spectral data compound 6a was assigned the following structure, corresponding to 5-(4-methoxybenzyl)-4-amino-1,2,4-triazole-3-ylnaphthalene-1-carbothioate (Figure 1):

Similarly, the other members of the series of 5-substituted-4-amino-1,2,4-triazole-3-thioester 6b-i were synthesized by the ring opening reaction due to the nucleophilic attack of hydrazine on the ring carbon of the 5-substituted-1,3,4-oxadiazole-2-thioesters, followed by dehydration. The ¹H-NMR and ¹³C-NMR, of the 5-substituted-1,3,4-oxadiazole-2-thioesters are outlined in

Table 1. ¹H-NMR spectral data of 5-substitued-4-amino-1,2,4-triazole-3-thioesters 6a-i.

Comp.	Protons [1H-δ(ppm), Multiplicity, J(Hz)]										Other protons
	Ar–H									NH2
	H-1	H-2	H-3	H-4	H-1′	H-2′	H-3′	H-4′	H-5′
6a	7.72 1H,dd 1.2,4.6	8.03 1H,dd 2.5,7.2	8.01 1H,dd 2.4,7.1	7.73 1H,dd 2.3,4.4	7.76 1H,m	7.42 1H,m	7.90 1H,m	7.88 1H,m	7.55 1H,m	4.32 2H,s	–
6b	7.60 1H,dd 3.1,4.2	7.77 1H,dd 3.2,4.1	7.04 1H,dd 2.5,6.6	7.45 1H,dd 4.2,3.1	7.88 1H,m	7.79 1H,m	7.62 1H,m	8.25 1H,m	7.21 1H,m	3.95 2H,s	–
6c	7.48 1H,m	7.32 1H,m	7.22 1H,m	7.97 1H,m	7.65 1H,dd 3.1,6.7	7.591H,dd1H,m	7.69 1H,dd 2.5,4.7	7.98 1H,dd 3.1,6.8	7.45 1H,dd 2.8,6.5	4.122H,s	–
6d	7.12 1H,m	7.22 1H,dd 2.4,6.7	7.08 1H,m	7.22 1H,dd 2.4,6.7	–	–	–	–	–	4.33 2H,s	2.88 4H,t 3.2 2H,t 2.1,1.5 10H,m
6e	7.14 1H,m	7.47 1H,m	7.12 1H,m	7.67 1H,m	7.97 1H,dd 2.4,6.5	7.58 1H,dd 3.1,4.5	7.77 1H,m	7.67 1H,m	–	4.16 2H,s	2.90 4H,t 2.8
6f	7.47 1H,dd 3.2,6.2	7.27 1H,dd 2.1,7.2	6.97 1H,dd 3.2,7.3	7.47 1H,dd 3.4,6.2	–	–	–	–	–	3.94 2H,s	2.50 2H,t 3.2,1.52 10H,m
6g	6.50 1H,m	6.87 1H,m	6.50 1H,m	6.87 1H,m	7.76 1H,dd 2.4,6.2	7.42 1H,dd 3.5,6.5	7.73 1H,m	7.46 1H,m	7.55 1H,m	3.83 2H,s	2.50 2H,s 3.56 3H,s
6h	6.52 1H,m	6.77 1H,m	7.14 1H,m	7.02 1H,m	–	–	–	–	–	4.45 2H,s	2.5 2H,t 3.2,1.60 12H,m
6i	7.12 1H,dd 2.3,6.4	7.27 1H,dd 3.2,7.4	7.12 1H,dd 2.3,6.4	7.77 1H,m	7.67 1H,m	7.85 1H,m	7.58 1H,m	7.82 1H,m	7.25 1H,m	4.26 2H,s	–

and Table 2 respectively.

In the in vitro antifungal bioassay, the compounds 6a, 6b, 6d and 6g were found to have significant antifungal activities against the four tested fungal strains (

Table 3. Antifungal activity of 5-substituted-4-amino-1,2,4-triazole-3-thioesters.

Sample No.	Structure of 5-substituted-4-amino-1,2,4-triazole-thioester	Fungal strains/ Inhibition (%)
		Aspergillus flavus	Mucor species	Aspergillus niger	Aspergillus flavus
6a		96.22	87.66	74.79	97.20
6b		84.12	100.00	81.20	100.00
6c		69.00	80.90	75.20	87.00
6d		81.00	78.36	59.19	93.60
6e		71.00	45.89	69.99	73.00
6f		27.00	15.58	24.39	75.00
6g		100.00	88.64	98.20	88.00
6h		54.00	21.22	20.00	52.00
6i		50.00	19.48	51.99	88.00
Terbinafine standard drug		100.00	90.00	110.80	98.40

). In particular 6a and 6g had high inhibitory effects on the growth of Aspergillus. flavus, Mucor species, Aspergillus niger and Aspergillus fumigatus. A structure activity relationship comparison shows that the presence of naphthyl group at the 3-position of the triazole-3-thioester enhances the activity of the compounds. It is interesting to note that a similar group is also present in the structure of the reference compound terbinafine.

The antifungal assay results also show that the activity of the compounds 6c, 6e, 6f, 6h and 6i is relatively low as compared to other members of the series due to the absence of this group in these structures. It is therefore concluded that the potential pharmacophore in the triazole-3-thioesters is probably naphthalene-1-carbothioate.

3. Experimental

3.1. General

Melting points of the synthesized compounds were recorded on Gallenkamp digital melting point apparatus MFB-595-101M in open-end capillary tubes and are uncorrected. Thin layer chromatography was carried out on pre-coated silica gel plates (0.2 mm, E. Merck, 20 × 20 cm, 60F₂₅₄). FTIR spectra were recorded on a Bio-Red Merlin Spectrophotometer using KBr discs.¹H-NMR (300 MHz) and ¹³C-NMR (75.43 MHz) spectra were recorded on Bruker AM-250 Spectrometer in DMSO and CDCl₃ solutions using TMS as internal standard. EIMS were recorded on Agilent VG: 70 SE Mass Spectrometer.

3.2. Procedure for the preparation of methyl-4-nitrobenzoate (1a)

4-Nitrobenzoic acid (5 g, 0.029 mol) was taken in a 250 mL round bottom flask fitted with a reflux condenser and a calcium chloride guard tube. Absolute methyl alcohol (25 mL) and few drops of concentrated sulphuric acid were added and the reaction mixture was refluxed for 4 hours. The reflux time was monitored by thin layer chromatography (tlc) (silica; ethyl acetate-pet. ether, 1:2). After the completion of the reaction the excess of alcohol was distilled off on a rotary evaporator. The residue was poured into 250 mL of water contained in a separating funnel. Dichloromethane (20 mL) was added to the separating funnel and the mixture was shaken vigorously. The solution was allowed to stand and the methyl-4-nitrobenzoate in the dichloromethane separated and settled at the bottom of the separating funnel. The lower layer was carefully separated and the upper aqueous layer was rejected. The methyl-4-nitrobenzoate was returned to the funnel and shaken with a strong solution of sodium bicarbonate until all the free acid was removed. Methyl-4-nitrobenzoate was washed once with water and dried by pouring into a small dry conical flask containing 2 g of anhydrous magnesium sulphate. It was shaken for 5 minutes and allowed to stand for one hour. The methyl-4-nitrobenzoate solution was filtered through a small fluted filter paper into a distillation flask. The flask was fitted with 360° thermometer, a condenser and a receiving flask. Dichloromethane was distilled off at 40 °C and the solid methyl-4-nitrobenzoate was obtained from the flask. Ethanol was used as solvent for recrystallization of the ester.

3.3. Procedure for the preparation of 4-nitrobenzoic acid hydrazide (2a)

Methyl-4-nitrobenzoate ester (7 g, 0.041 mol) was dissolved in absolute ethanol (40 mL) and taken in a flask fitted with a reflux condenser and a calcium chloride guard tube. Hydrazine hydrate (80%, 13 mL) was added and the reaction mixture was refluxed for eight hours. The reflux time was monitored by tlc as before. After the completion of the reaction, the excess hydrazine was distilled off. The crude solid was collected, washed with water and recrystallized from 30% aqueous ethanol.

3.4. Procedure for the preparation of 5-(4-nitrophenyl)-1,3,4-oxadiazole-2-thiol (3a)

4-Nitrobenzoic hydrazide (7 g, 0.038 mol) was dissolved in absolute ethanol (40 mL) in a 250 mL flask. Carbon disulfide (2 mL, 0.034 mol) was then added to the solution followed by the addition of a solution of potassium hydroxide (1.2 g, 0.019 mol) in water (20 mL). The reaction mixture was thoroughly stirred and refluxed. It was initially yellow which turned to green and then light yellow with the progress of the reaction. Evolution of hydrogen sulfide gas was observed during each reaction. After completion of the reaction, excess of ethanol was removed under reduced pressure. The mixture was diluted with distilled water (200 mL) and acidified with 4N hydrochloric acid to pH 2-3. It was then filtered, washed with diethyl ether and recrysallized from ethanol.

3.5. Procedure for the preparation of 5-(4-nitrophenyl)-4-amino-1,2,4-triazole-3-thiol (4a)

5-(4-Nitrophenyl)-1,3,4-oxadiazole-2-thiol (4 g, 0.017 mol) and 80% hydrazine hydrate (6 mL, 0.124 mol) in absolute ethanol (25) were refluxed in a 250 mL flask fitted with a condenser and a guard tube. After the completion of the reaction the solvent and excess of hydrazine hydrate were removed under reduced pressure using rotary evaporator. The residue was washed with diethyl ether and recrystallized from ethanol.

3.6. Procedure for the preparation of 5-(4-methoxybenzyl)-1,3,4-oxadiazole-2-ylnaphthalene-1-carbothioate (5a)

5-(4-Methoxybenzyl)-1,3,4-oxadiazole-2-thiol (5 g, 0.021 mol) was dissolved in absolute ethanol (30 mL) in a 250 mL flask. Potassium hydroxide (1 g, 0.178 mol) solution in water (20 mL) was added to the solution. After one hour reflux 1-naphthoyl chloride (4 g, 0.021 mol) was added to the reaction mixture. The reaction mixture was thoroughly stirred and refluxed for six hours. After completion of the reaction, the excess of ethanol in the reaction mixture was under reduced pressure. The mixture left behind was diluted with distilled water (200 mL) and then filtered. The crude product was dried in an oven and recrystallized from ethanol.

3.7. Preparation for the preparation of 5-(4-methoxybenzyl)-4-amino-1,2,4-triazole-3-ylnaphthalene-1-carbothioate (6a).

5-(4-Methoxybenzyl)-1,3,4-oxadiazole-2-ylnaphthalene-1-carbothioate (5 g, 0.013 mol) and 80% hydrazine hydrate (8 mL, 0.165 mol) in absolute ethanol (30 mL) were refluxed in a 250 mL flask fitted with a condenser and a guard tube. After completion of the reaction, the solvent and excess hydrazine hydrate were removed under reduced pressure. The residue was washed with diethyl ether and recrystallized from ethanol.

Purification of all the synthesized compounds was achieved by recrystallization and purity of each compound was monitored by thin layer (tlc) and gas (gc) chromatography.

5-(4-Nitrophenyl)-4-amino-1,2,4-triazole-3-ylnaphthalene-1-carbothioate (6a). 5-(4-Nitrophenyl)-1,3,4-oxadiazole-2-yl naphthalene-1-carbothioate (3.0 g; 8 mmol), NH₂-NH₂ (5.10 g; 103 mmol) were reacted according to the general procedure. Color light brown; yield: 80% (2.40 g); recrystallization from EtOH; m.p. 168-170 °C; Molecular formula C₁₉H₁₃O₃N₅S; Mol. Wt. 191. FTIR (KBr, cm^-1): 3250 (N-H), 1557 (C=C)_Ar, 1633 (C=O), 1517 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 7.72 (dd, 1H, J = 1.2, 4.6 Hz, ArH-1), 8.03 (dd, 1H, J = 2.5, 7.2 Hz, Ar H-2) 8.01 (dd, 1H, J = 2.4, 7.1 Hz, A H-3), 7.73 (dd, 1H, J = 2.3, 4.4 Hz, Ar H-4), 7.76 (m, 1H, Ar H-1´ ),7.42 (m, 1H, Ar H-2´ ), 7.90 (m, 1H, Ar H-3´ ), 7.88 (m, 1H, Ar H-4´ ), 7.55 (m, 1H, Ar H-5´ ), 4.32 (s, 2H, NH₂); ¹³C-NMR (DMSO-d₆, δ, ppm): 129.41 (C-1), 128.40 (C-2), 116.52 (C-3), 115.52 (C-4), 116.31 (C-5), 130.43 (C-6), 160.42 (C-7), 171.40 (C-8), 182.31 (C-9), 135.61 (C-10), 127.12 (C-11), 120.43 (C-12), 122.41(C-13), 125.32 (C-14), 126.32 (C-15), 128.43 (C-16), 129.42 (C-17), 130.21 (C-18), 129.66 (C-19); MS (m/z) 391 (40%), (M⁺), 361 (35%), 243 (50%), 155 (100%; loss of naphthoyl fragment), 148 (60%), 136 (65%), 127 (55%; loss of naphthoyl fragment – CO).

5-(3-Nitrophenyl)-4-amino-1,2,4-triazole-3-ylnaphthalene-1-carbothioate (6b). 5-(3-Nitrophenyl)-1,3,4-oxadiazole-2-ylnaphthalene-1-carbothioate (4.5 g; 12 mmol), NH₂-NH₂ (6.20 g; 124 mmol) were reacted according to the general procedure. Color light yellow; yield: 83% (3.80 g); recrystallization from EtOH; m.p. 155-157 °C; Molecular formula C₁₉H₁₃O₃N₅S; Mol. Wt. 191. FTIR (KBr, cm^-1): 3202 (N-H), 1566 (C=C)_Ar, 1655 (C=O), 1519 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 7.60 (dd, 1H, J = 3.1, 4.2 Hz, ArH-1), 7.77 (dd, 1H, J = 3.2, 4.1 Hz, Ar H-2), 7.04 (dd, 1H, J = 2.5, 6.6Hz, A H-3), 7.45 (dd, 1H, J = 4.2, 3.1 Hz, Ar H-4), 7.88 (m, 1H, Ar H-1´), 7.79 (m, 1H, Ar H-2´ ), 7.62 (m, 1H, Ar H-3´ ), 8.25 (m, 1H, Ar H-4´ ), 7.21 (m, 1H, Ar H-5´ ), 3.95 (s, 2H, NH₂); ¹³C-NMR (DMSO-d₆, δ, ppm): 128.40 (C-1), 127.41 (C-2), 155.50 (C-3), 116.62 (C-4), 118.62 (C-5), 127.42 (C-6), 162.61 (C-7), 174.65 (C-8), 180.37 (C-9), 136.23(C-10), 129.12 (C-11),122.31 (C-12), 125.71(C-13), 128.81 (C-14), 127.50 (C-15), 130.64 (C-16), 132.80 (C-17), 126.21 (C-18), 129.44 (C-19); MS (m/z) 391 (40%), (M⁺), 361 (35%), 243 (50%), 155 (100%; loss of naphthoyl fragment) 148 (60%), 136 (65%), 127 (55%; loss of naphthoyl fragment - CO).

5-Phenyl-4-amino-1,2,4-oxadiazole-3-ylbenzothioate (6c). 5-Phenyl-1,3,4-oxadiazole-2-yl benzo-thioate (4.0 g; 14 mmol), NH₂-NH₂ (5.60 g; 113 mmol) were reacted according to the general procedure. Color red; yield: 78% (3.10 g); recrystallization from EtOH; m.p. 214-215 °C; Molecular formula C₁₅H₁₂ON₄S; Mol. Wt. 296. FTIR (KBr, cm^-1): 3212 (N-H), 1574 (C=C)_Ar, 1664 (C=O), 1525 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 7.48 (m, 1H, ArH-1) 732 (m, 1H, Ar H-2), 7.22 (m, 1H, A H-3) 7.97 (m, 1H, Ar H-4), 7.65 (dd, 1H, J = 3.1, 6.7 Hz, Ar H-1´ ), 7.59 (dd, 1H, J = 3.2, 4.4 Hz, Ar H-2´), 7.69 (dd, 1H, J = 2.5, 4.7 Hz, Ar H-3´ ), 7.90 (dd, 1H, J = 3.1, 6.8 Hz, Ar H-4´ ), 7.45 (dd, 1H, J = 2.8, 6.5 Hz, Ar H-5´ ), 4.12 (s, 2H, NH₂); ¹³C-NMR (DMSO-d₆, δ, ppm): 130.05 (C-1), 126.41 (C-2), 116.24 (C-3), 115.24 (C-4), 116.37 (C-5), 126.21 (C-6), 166.04 (C-7), 176.23 (C-8), 184.32 (C-9), 134.05 (C-10), 130.73 (C-11),126.32 (C-12), 125.61 (C-13), 126.67 (C-14), 130.73 (C-15); MS (m/z) 296 (42%) (M⁺), 266 (35%), 193 (50%), 103 (62%),105 (100%; loss of acylium ion fragment), 77 (52%; loss of phenyl cation fragment).

5-Phenethyl-4-amino-1,2,4-triiazole-3-yloctanethioate (6d). 5-Phenethyl-1,3,4-oxadiazole-2-yl octane-thioate (6.0 g; 18 mmol), NH₂-NH₂ (8.20 g; 165 mmol) were reacted according to the general procedure. Color orange; yield: 74% (4.40 g); recrystallization from EtOH; m.p. 179-180 °C; Molecular formula C₁₈H₂₆ON₄S; Mol. Wt. 346. FTIR (KBr, cm^-1): 3210 (N-H), 1520 (C=C)_Ar, 1660 (C=O), 1582 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm):; ¹HNMR (DMSO-d₆) δ (ppm): 7.12 (m, 1H, ArH-1), 7.22 (dd, 1H, J = 2.4, 6.7 Hz, ArH-2), 7.08 (m, 1H, ArH-3), 7.22 (dd, 1H, J = 2.4, 6.7 Hz, ArH-2), 4.33 (s, 2H, NH₂), 2.88, 3.2, 1.5 (t, 4H, t, 2H, m, 10 H other protons) 2.50, 3.2, 1.52 (t, 2H, m, 10H, other protons); ¹³C-NMR (DMSO-d₆, δ, ppm): 28.36 (C-1), 30.18 (C-2), 127.21 (C-3), 126.33 (C-4), 127.41 (C-5), 116.24 (C-6), 118.44 (C-7), 116.26 (C-8), 166.23 (C-9), 175.90(C-10), 182.64 (C-11),45.46 (C-12), 33.67(C-13), 26.34 (C-14), 25.80 (C-15), 20.21 (C-16), 18.44 (C-17), 22.37 (C-18); MS (m/z) 346 (40%) (M⁺), 316 (36%), 215 (50%), 131 (60%), 127 (100%), 99 (55%).

5-Phenethyl-4-amino-1,2,4-triazole-3-ylbenzothioate (6e). 5-Phenethyl-1,3,4-oxadiazole-2-yl benzo-thioate (5.0 g; 16 mmol), NH₂-NH₂ (7.20 g; 144 mmol) were reacted according to the general procedure. Color light yellow; yield: 80% (4.00 g); recrystallization from EtOH; m.p. 158-159 °C; Molecular formula C₁₇H₁₆ON₄S; Mol. Wt. 324. FTIR (KBr, cm^-1): 3195 (N-H), 1509 (C=C)_Ar, 1673 (C=O), 1567 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 7.14 (m, 1H, ArH-1), 7.47 (dd, 1H, J = 2.1, 7.2 Hz, Ar H-2), 7.12 (m, 1H, ArH-3), 7.67 (m, 1H, Ar H-4), 7.97 dd, 1H, J = 2.4, 6.5 Hz, ArH-1´), 7.58 (dd, 1H J = 3.1, 4.5 Hz, Ar-H-2´), 7.77 (m, 1H ArH-3´), 7.67 (m, 1H ArH-4´) 4.16 (s, 2H, NH₂), 2.90 (t, 4H other protons); ¹³C-NMR (DMSO-d₆, δ, ppm): 30.33 (C-1), 32.71 (C-2), 130.43 (C-3), 118.23 (C-4), 117.23 (C-5), 115.48 (C-6), 118.23 (C-7), 125.63 (C-8), 159.21 (C-9), 170.62 (C-10), 183.47 (C-11),134.24 (C-12), 128.40 (C-13), 129.65 (C-14), 126.31 (C-15), 130.52 (C-16), 127.43 (C-17); MS (m/z) 324 (45%) (M⁺), 294 (35%), 193 (50%), 131 (62%), 105 (100%; loss of acylium ion fragment), 77 (52%; loss of phenyl cation fragment).

5-(3-Nitrophenyl)-4-amino-1,2,4-triazole-3-ylheptanethioate (6f). 5-(3-Nitrophenyl)-1,3,4-oxadiazole-2-ylheptanethioate (3.0g; 8 mmol), NH₂-NH₂ (5.10 g; 103 mmol) were reacted according to the general procedure. Color golden; yield: 76% (2.30 g); recrystallization from EtOH; m.p. 148-150 °C; Molecular formula C₁₅H₁₉O₃N₅S; Mol. Wt. 349. FTIR (KBr, cm^-1): 3140 (N-H), 1570 (C=C)_Ar, 1667 (C=O), 1514 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 7.47 (dd, 1H, J = 3.2, 6.2 Hz, ArH-1), 7.27 (dd, 1H, J = 2.1, 7.2 Hz, Ar H-2), 6.97 (dd, 1H, J = 3.2, 7.3 Hz, Ar H-3), 7.47 (dd, 1H, J = 3.4, 6.2 Hz, Ar H-4), 3.94 (s, 2H, NH₂), 2.50, 3.2, 1.52 (t, 2H m, 10H, other protons); ¹³C-NMR (DMSO-d₆, δ, ppm): 130.06 (C-1), 127.25 (C-2), 152.44 (C-3), 127.25 (C-4), 126.31 (C-5), 117.21 (C-6), 161.37 (C-7), 170.68 (C-8), 182.04 (C-9), 54.34 (C-10), 44.31 (C-11),33.39 (C-12), 24.36 (C-13), 25.62 (C-14), 20.22 (C-15); MS (m/z) 349 (40%) (M⁺), 319 (37%), 201 (50%), 148 (60%),113 (100%), 85 (55%).

5-(4-Methoxybenzyl)-4-amino-1,2,4-triazole-3-ylnaphthalene-1-carbothioate (6g). 5-(4-Methoxy-benzyl)-1,3,4-oxadiazole-2-yl naphthalene-1-carbothioate (5.0 g; 13 mmol), NH₂-NH₂ (8.20 g; 165 mmol) were reacted according to the general procedure. Color reddish brown; yield: 82% (4.10 g); recrystallization from EtOH; m.p. 166-167 °C; Molecular formula C₂₁H₁₈O₂N₄S; Mol. Wt. 390. FTIR (KBr, cm^-1): 3204 (N-H), 1575 (C=C)Ar, 1684 (C=O), 1518 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 6.50 (m, 1H, ArH-1), 6.87 (m, 1H, Ar H-2), 6.50 (m, 1H, A H-3), 6.87 (m, 1H, Ar H-4), 7.56 (dd, 1H, J = 2.4, 6.2 Hz, Ar H-1´ ), 7.42 (dd, 1H, J = 3.5, 6.5 Hz, Ar H-2´ ), 7.73 (m, 1H, Ar H-3´ ), 7.46 (m, 1H, Ar H-4´ ), 7.55 (m, 1H, Ar H-5´ ), 3.83 (s, 2H, NH₂), 2.50, (s, 2H, methylene protons) 3.56 (s, 3H, methyl protons); ¹³C-NMR (DMSO-d₆, δ, ppm): 30.60 (C-1), 55.90 (C-2), 130.02 (C-3), 127.05 (C-4), 116.65 (C-5), 158.03 (C-6), 166.04 (C-7), 175.10 (C-8), 180.01 (C-9), 116.65 (C-10), 127.05 (C-11),134.50 (C-12), 127.12 (C-13), 125.62 (C-14), 120.50 (C-15), 119.42 (C-16), 129.31 (C-17), 131.61 (C-18), 130.71 (C-19), 123.63 (C-20); MS (m/z) 390 (40%) (M⁺), 360 (37%), 243 (50%), 147 (60%), 155 (100%; loss of naphthoyl fragment) 148 (60%), 136 (65%), 127 (55%; loss of naphthoyl fragment - CO) 121 (65%; loss of tropylium ion).

5-Phenethyl-4-amino-1,2,4-triazole-3-ylnonanethioate (6h). 5-Phenethyl-1,3,4-oxadiazole-2-yl nonane-thioate (4.0 g; 12 mmol), NH₂-NH₂ (5.60 g; 113 mmol) were reacted according to the general procedure. Color yellow; yield: 78% (3.10 g); recrystallization from EtOH; m.p. 138-139 °C; Molecular formula C₁₇H₂₄ON₄S; Mol. Wt. 332. FTIR (KBr, cm^-1): 3260 (N-H), 1512 (C=C)_Ar, 1652 (C=O), 1590 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 6.52.(m, 1H, ArH-1), 6.77 (m, 1H, Ar H-2), 7.14 (m, 1H, A H-3), 7.02 (m, 1H, Ar H-4), 4.45 (s, 2H, NH₂), 2.50, 3.2, 1.60 (t, 2H, m, 12H other protons); ¹³C-NMR (DMSO-d₆, δ, ppm): 130.46 (C-1), 125.34 (C-2), 116.73 (C-3), 118.62 (C-4), 116.73 (C-5), 125.23 (C-6), 159.68 (C-7), 168.77 (C-8), 184.66 (C-9), 53.62 (C-10), 44.02 (C-11),34.71 (C-12), 29.01 (C-13), 25.72 (C-14), 25.69 (C-15), 20.02 (C-16), 18.33 (C-17). MS (m/z) 332 (40%) (M⁺), 302 (37%), 229 (52%), 141 (100%), 113 (50%), 103 (58%)

5-(3-Nitrophenyl)-4-amino-1,2,4-triazole-3-ylbenzothioate (6i). 5-(3-Nitrophenyl)-1,3,4-oxadiazole-2-ylbenzothioate (3.0 g; 9 mmol), NH₂-NH₂ (5.10 g; 103 mmol) were reacted according to the general procedure. Color brown; yield: 66% (2.10 g); recrystallization from EtOH; m.p. 138-139 °C; Molecular formula C₁₅H₁₁O₃N₅S; Mol. Wt. 341. FTIR (KBr, cm^-1): 3318 (N-H), 1510 (C=C)_Ar, 1625 (C=O), 1587 (C=N); ¹H-NMR (DMSO-d₆, δ, ppm): 7.12 (dd, 1H, J = 3.2, 6.4 Hz, ArH-1),7.27 (dd, 1H, J = 3.2, 7.4 Hz, Ar H-2), 7.12 (dd, 1H, J = 3.2, 6.4 Hz, A H-3), 7.77 (m, 1H, Ar H-4), 7.67 (m, 1H, Ar H-1´), 7.85 (m, 1H, Ar H-2´), 7.58 (m, 1H, Ar H-3´), 7.82 (m, 1H, Ar H-4´), 7.25 (m, 1H, Ar H-5´), 4.26 (s, 2H, NH₂); ¹³C-NMR (DMSO-d₆, δ, ppm): 133.06 (C-1), 127.45 (C-2), 153.44 (C-3), 125.21 (C-4), 127.40 (C-5), 117.67 (C-6), 163.23 (C-7), 177.45 (C-8), 181.08 (C-9), 136.38 (C-10), 127.21 (C-11),116.90 (C-12), 126.64 (C-13), 128.03 (C-14), 125.64 (C-15); MS (m/z) 341 (42%) (M⁺), 311 (35%), 193 (50%), 148 (60%), 105 (100%; loss of acylium ion fragment), 77 (52%; loss of phenyl cation fragment).

3.8. Antifungal evaluation

The synthesized compounds were tested by agar tube dilution method [17] for their in vitro fungicidal activity. Terbinafine (200 μg/mL) was used as positive control. All experiments were done in three replicates. Four fungal strains: Aspergillus flavus, Mucor species, Aspergillus niger and Aspergillus fumigatus were used. All fungal strains were grown on 6.5% SDA (Sabouraud dextrose agar, pH 5.7) at 28 °C and preserved at 4 °C in a refrigerator. One hundred mm slants with sterilized SDA were prepared by adding each compound in 200 μg/mL concentration. Terbinafine (200 μg/mL) was used as standard drug while DMSO was used as negative control. Each slant was inoculated with 4 mm piece of respective fungal strain and incubated at 28 °C for 7-10 days. Fungal growth was compared with negative control to get the %age inhibition.

4. Conclusions

Nine 5-substitued-1,3,4-oxadiazole-2-thioesters derivatives 5a-i containing different groups and obtained via 5-substituted-1,3,4-oxadiazole-2-thiols using potassium hydroxide and acid chloride were subjected to reactions with hydrazine hydrate to furnish nine new 5-substituted-4-amino-1,2,4-triazole-3-thioesters 6a-i.Their structures were confirmed by infrared, ¹H- and ¹³C -NMR and mass spectrometric analysis. In the in vitro antifungal bioassay, compounds 6a, 6b, 6d and 6g were found to have significant antifungal activities against the four tested fungal strains: Aspergillus flavus, Mucor species, Aspergillu niger and Aspergillu. fumigatus Structure activity relationship comparison shows that the presence of naphthyl group at 3-position of the triazole-3-thioester enhances the activity of the compounds, probably because a similar group is also present in the structure of the reference compound terbinafine.