Identification of Dehydroxytrichostatin A as a Novel Up-Regulator of the ATP-Binding Cassette Transporter A1 (ABCA1)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Identification of 9179B from Strain 04-9179
Position | 13C-NMR a | 1H-NMR b |
---|---|---|
1 | n.d. c | |
2 | 120.1 | 5.97 (1H, d, J = 15.5) |
3 | 146.9 | 7.10 (1H, d, J = 15.5) |
4 | 134.3 | |
5 | 141.1 | 5.86 (1H, d, J = 9.5) |
6 | 41.6 | 4.48 (1H, m) |
7 | 201.4 | |
8 | 124.6 | |
9,13 | 131.8 | 7.81 (2H, d, J = 9.0) |
10,12 | 111.9 | 6.67 (2H,d, J = 9.0) |
11 | 155.4 | |
4-Me | 12.7 | 1.80 (3H, s) |
6-Me | 18.2 | 1.22 (3H, d, J = 6.5) |
N-Me2 | 40.2 | 3.02 (6H, s) |
2.2. Effect of 9179B and Other HDAC Inhibitors on ABCA1 Promoter Activity
2.3. The Effects of 9179B on ABCA1 Expression in HepG2 and RAW 264.7 Cells and on Cholesterol Efflux in RAW 264.7 Cells
2.4. The Influence of 9179B on SR-BI and the Uptake of DiI-HDL in RAW 264.7 Cells
2.5. The Influence of 9179B on Different Cis-Regulatory Elements of ABCA1
3. Experimental
3.1. General
3.2. Cell Culture
3.3. The Isolation, Purification and Structure Elucidation of 9179B
3.4. ABCA1 Promoter Activity Assay
3.5. Enzyme Inhibitor Activity Assay and Other HDAC Inhibitor on ABCA1 Promoter Activity
3.6. The Effects of 9179B on ABCA1 Expression in HepG2 or RAW264.7 Cells
3.7. The Effects of 9179B on Cholesterol Efflux in RAW 264.7 Cells
3.8. The Effect of 9179B on SR-BI Expression in RAW264.7 Cells and the Analysis of Cellular Uptake of DiI-Labeled HDL by Flow Cytometry
3.9. Luciferase Reporter Assay
3.10. Statistical Analysis
4. Conclusions
Acknowledgments
- Sample Availability: Samples of the compounds are available from the authors and commercially available.
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Xu, Y.; Xu, Y.; Bao, Y.; Hong, B.; Si, S. Identification of Dehydroxytrichostatin A as a Novel Up-Regulator of the ATP-Binding Cassette Transporter A1 (ABCA1). Molecules 2011, 16, 7183-7198. https://doi.org/10.3390/molecules16097183
Xu Y, Xu Y, Bao Y, Hong B, Si S. Identification of Dehydroxytrichostatin A as a Novel Up-Regulator of the ATP-Binding Cassette Transporter A1 (ABCA1). Molecules. 2011; 16(9):7183-7198. https://doi.org/10.3390/molecules16097183
Chicago/Turabian StyleXu, Yang, Yanni Xu, Yi Bao, Bin Hong, and Shuyi Si. 2011. "Identification of Dehydroxytrichostatin A as a Novel Up-Regulator of the ATP-Binding Cassette Transporter A1 (ABCA1)" Molecules 16, no. 9: 7183-7198. https://doi.org/10.3390/molecules16097183