A Novel Anti-Inflammatory Role for Ginkgolide B in Asthma via Inhibition of the ERK/MAPK Signaling Pathway
Abstract
:1. Introduction
2. Results and Discussion
2.1. GKB Reduces Ovalbumin-induced Bronchoalveolar Lavage Fluid T Helper Type 2 Cytokine Levels
2.2. GKB Reduces OVA-Induced Serum Levels of OVA-specific IgE
2.3. GKB Reduces OVA-Induced Bronchoalveolar Lavage Fluid (BALF) Inflammatory Cell Recruitment
2.4. Effects of GKB on OVA-Induced Airway Hyper-Responsiveness
2.5. Effects of GKB on OVA-Induced Airway Goblet Cell Hyperplasia and Mucus Production
2.6. Effects of GKB on Activation of p38, ERK and JNK
2.7. Discussion
3. Experimental
3.1. Animals
3.2. Reagent
3.3. Antigen Sensitization, Challenge and GKB Treatment
3.4. BALF and Serum Collection
3.5. Cytokine Levels in Lung Tissues
3.6. Mouse Anti-OVA IgE ELISA
3.7. Determination of Airway Hyper-Responsiveness
3.8. Histological Examination
3.9. Western Blot Analysis
3.10. Statistical Analysis
4. Conclusions
Acknowledgments
- Samples Availability: Samples of the compounds are available from the authors (or from MDPI).
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Chu, X.; Ci, X.; He, J.; Wei, M.; Yang, X.; Cao, Q.; Li, H.; Guan, S.; Deng, Y.; Pang, D.; et al. A Novel Anti-Inflammatory Role for Ginkgolide B in Asthma via Inhibition of the ERK/MAPK Signaling Pathway. Molecules 2011, 16, 7634-7648. https://doi.org/10.3390/molecules16097634
Chu X, Ci X, He J, Wei M, Yang X, Cao Q, Li H, Guan S, Deng Y, Pang D, et al. A Novel Anti-Inflammatory Role for Ginkgolide B in Asthma via Inhibition of the ERK/MAPK Signaling Pathway. Molecules. 2011; 16(9):7634-7648. https://doi.org/10.3390/molecules16097634
Chicago/Turabian StyleChu, Xiao, Xinxin Ci, Jiakang He, Miaomiao Wei, Xiaofeng Yang, Qingjun Cao, Hongyu Li, Shuang Guan, Yanhong Deng, Daxin Pang, and et al. 2011. "A Novel Anti-Inflammatory Role for Ginkgolide B in Asthma via Inhibition of the ERK/MAPK Signaling Pathway" Molecules 16, no. 9: 7634-7648. https://doi.org/10.3390/molecules16097634