NADPH Oxidase-Derived Reactive Oxygen Species Are Involved in the HL-60 Cell Monocytic Differentiation Induced by Isoliquiritigenin
Abstract
:Abbreviations
AO | acridine orange |
APO | apocynin |
BSO | L-buthionine-(S,R)-sulfoximine |
DCF-DA | 2′,7′-dichlorofluorescein diacetate |
DMSO | dimethyl sulfoxide |
DPI | diphenyliodonium |
ES | embryonic stem |
HL-60 | human promyelocytic leukemia cells |
ISL | Isoliquiritigenin |
MFI | mean fluorescence intensity |
NAC | N-acetyl-L-cysteine |
NADPH | nicotinamide adenine dinucleotide phosphate |
NBT | Nitrobluetetrazolium |
PBS | phosphate buffered saline |
PCR | Polymerase Chain Reaction |
ROS | reactive oxygen species |
SMC | smooth muscle cell |
SOD | superoxide dismutase |
Tempol | 4-hydroxy-2,2,6,6-tetramethyl piperidinoxyl |
TPA | 12-O-tetradecanoylphorbol 13-acetate |
1. Introduction
2. Results and Discussion
2.1. ISL Induced Differentiation in HL-60 Cells
2.2. ROS Is Closely Related to Cell Differentiation Induced by ISL
2.3. NADPH Oxidase Is Involved in Cell Differentiation Induced by ISL
2.4. Discussion
3. Experimental
3.1. Materials
3.2. Cell Culture and Treatments
3.3. Determination of Cell Differentiation
3.4. Determination of Intracellular ROS
3.5. Western Blotting
3.6. RNA Extraction and Real-Time RT-PCR
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
- Sample Availability: Samples of the isoliquiritigenin are available from the authors.
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Chen, H.; Zhang, B.; Yao, Y.; Chen, N.; Chen, X.; Tian, H.; Wang, Z.; Zheng, Q. NADPH Oxidase-Derived Reactive Oxygen Species Are Involved in the HL-60 Cell Monocytic Differentiation Induced by Isoliquiritigenin. Molecules 2012, 17, 13424-13438. https://doi.org/10.3390/molecules171113424
Chen H, Zhang B, Yao Y, Chen N, Chen X, Tian H, Wang Z, Zheng Q. NADPH Oxidase-Derived Reactive Oxygen Species Are Involved in the HL-60 Cell Monocytic Differentiation Induced by Isoliquiritigenin. Molecules. 2012; 17(11):13424-13438. https://doi.org/10.3390/molecules171113424
Chicago/Turabian StyleChen, Hongmei, Bo Zhang, Ying Yao, Na Chen, Xiaoyu Chen, Hui Tian, Zhenhua Wang, and Qiusheng Zheng. 2012. "NADPH Oxidase-Derived Reactive Oxygen Species Are Involved in the HL-60 Cell Monocytic Differentiation Induced by Isoliquiritigenin" Molecules 17, no. 11: 13424-13438. https://doi.org/10.3390/molecules171113424